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Protein

DNA ligase

Gene

lig

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Interaction with PCNA enhances ligase activity. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

a divalent metal cation1 Publication

Enzyme regulationi

Ligase activity stimulated by PCNA heterotrimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei258ATP1
Active sitei260N6-AMP-lysine intermediate1
Binding sitei265ATP1
Binding sitei280ATP1
Binding sitei310ATPUniRule annotation1
Binding sitei350ATPUniRule annotation1
Binding sitei427ATP1
Binding sitei433ATP1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.5.1.1. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:SSO0189
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 30Missing : No interaction with PCNA3, no stimulation by PCNA heterotrimer. 1 PublicationAdd BLAST30
Mutagenesisi110 – 111FL → AA: Impairs interaction with PCNA. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596181 – 601DNA ligaseAdd BLAST601

Proteomic databases

PRIDEiQ980T8.

Interactioni

Subunit structurei

Interacts with the PCNA heterotrimer, probably via subunit PCNA3.2 Publications

Protein-protein interaction databases

STRINGi273057.SSO0189.

Structurei

Secondary structure

1601
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Helixi19 – 31Combined sources13
Helixi35 – 37Combined sources3
Helixi38 – 44Combined sources7
Turni45 – 47Combined sources3
Helixi52 – 54Combined sources3
Helixi63 – 74Combined sources12
Helixi78 – 88Combined sources11
Helixi91 – 100Combined sources10
Helixi121 – 133Combined sources13
Helixi139 – 153Combined sources15
Helixi156 – 166Combined sources11
Helixi176 – 187Combined sources12
Beta strandi188 – 190Combined sources3
Helixi191 – 193Combined sources3
Helixi194 – 203Combined sources10
Helixi207 – 217Combined sources11
Helixi219 – 222Combined sources4
Beta strandi237 – 240Combined sources4
Helixi244 – 250Combined sources7
Turni251 – 253Combined sources3
Beta strandi255 – 260Combined sources6
Beta strandi262 – 271Combined sources10
Beta strandi274 – 278Combined sources5
Helixi286 – 288Combined sources3
Helixi290 – 299Combined sources10
Beta strandi303 – 314Combined sources12
Turni316 – 318Combined sources3
Helixi325 – 332Combined sources8
Helixi336 – 342Combined sources7
Beta strandi345 – 355Combined sources11
Helixi365 – 375Combined sources11
Beta strandi380 – 384Combined sources5
Beta strandi387 – 392Combined sources6
Helixi393 – 405Combined sources13
Beta strandi410 – 414Combined sources5
Beta strandi427 – 435Combined sources9
Helixi436 – 439Combined sources4
Beta strandi440 – 442Combined sources3
Beta strandi444 – 455Combined sources12
Helixi458 – 460Combined sources3
Beta strandi463 – 473Combined sources11
Turni474 – 477Combined sources4
Beta strandi478 – 485Combined sources8
Helixi491 – 502Combined sources12
Beta strandi505 – 508Combined sources4
Beta strandi520 – 523Combined sources4
Beta strandi528 – 539Combined sources12
Turni544 – 548Combined sources5
Beta strandi554 – 559Combined sources6
Beta strandi561 – 565Combined sources5
Helixi571 – 573Combined sources3
Helixi577 – 586Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIVX-ray2.05A1-601[»]
2HIXX-ray2.87A1-601[»]
ProteinModelPortaliQ980T8.
SMRiQ980T8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ980T8.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
InParanoidiQ980T8.
KOiK10747.
OMAiPQVVIEV.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q980T8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKTIIDK VVYIIQGKLW
60 70 80 90 100
PDFLGYPELG IGEKFLIKAI SIATNTDENS VENLYKTIGD LGEVARRLKS
110 120 130 140 150
KQQSTGILGF LGTTSKESLT VDEVYSTLSK VALTTGEGSR DLKIRLLAGL
160 170 180 190 200
LKKADPLEAK FLVRFVEGRL RVGIGDATVL DAMAIAFGGG QSASEIIERA
210 220 230 240 250
YNLRADLGNI AKIIVEKGIE ALKTLKPQVG IPIRPMLAER LSNPEEILKK
260 270 280 290 300
MGGNAIVDYK YDGERAQIHK KEDKIFIFSR RLENITSQYP DVVDYVSKYI
310 320 330 340 350
EGKEFIIEGE IVAIDPESGE MRPFQELMHR KRKSDIYEAI KEYPVNVFLF
360 370 380 390 400
DLMYYEDVDY TTKPLEARRK LLESIVKPND YVKIAHHIQA NNVEDLKSFF
410 420 430 440 450
YRAISEGGEG VMVKAIGKDA IYQAGARGWL WIKLKRDYQS EMADTVDLVV
460 470 480 490 500
VGGFYGKGKR GGKISSLLMA AYNPKTDSFE SVCKVASGFS DEQLDELQKK
510 520 530 540 550
LMEIKRDVKH PRVNSKMEPD IWVEPVYVAE IIGSEITISP LHTCCQDVVE
560 570 580 590 600
KDAGLSIRFP RFIRWRDDKS PEDATTTDEI LEMYNKQPKK KIESPAVDES

V
Length:601
Mass (Da):67,733
Last modified:October 1, 2001 - v1
Checksum:iDA6814F4A6F0546E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40535.1.
PIRiH90159.
RefSeqiWP_009990427.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40535; AAK40535; SSO0189.
GeneIDi27426483.
KEGGisso:SSO0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40535.1.
PIRiH90159.
RefSeqiWP_009990427.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIVX-ray2.05A1-601[»]
2HIXX-ray2.87A1-601[»]
ProteinModelPortaliQ980T8.
SMRiQ980T8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0189.

Proteomic databases

PRIDEiQ980T8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40535; AAK40535; SSO0189.
GeneIDi27426483.
KEGGisso:SSO0189.

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
InParanoidiQ980T8.
KOiK10747.
OMAiPQVVIEV.

Enzyme and pathway databases

BRENDAi6.5.1.1. 6163.

Miscellaneous databases

EvolutionaryTraceiQ980T8.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI_SULSO
AccessioniPrimary (citable) accession number: Q980T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: October 1, 2001
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.