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Q980T8 (DNLI_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:SSO0189
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Interaction with PCNA enhances ligase activity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations. Ref.2

Subunit structure

Interacts with the PCNA heterotrimer. Ref.2

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601DNA ligase HAMAP-Rule MF_00407
PRO_0000059618

Sites

Active site2601N6-AMP-lysine intermediate
Binding site2581ATP
Binding site2651ATP
Binding site2801ATP
Binding site3101ATP By similarity
Binding site3501ATP By similarity
Binding site4271ATP
Binding site4331ATP

Experimental info

Mutagenesis110 – 1112FL → AA: Impairs interaction with PCNA. Ref.2

Secondary structure

................................................................................................ 601
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q980T8 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: DA6814F4A6F0546E

FASTA60167,733
        10         20         30         40         50         60 
MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKTIIDK VVYIIQGKLW PDFLGYPELG 

        70         80         90        100        110        120 
IGEKFLIKAI SIATNTDENS VENLYKTIGD LGEVARRLKS KQQSTGILGF LGTTSKESLT 

       130        140        150        160        170        180 
VDEVYSTLSK VALTTGEGSR DLKIRLLAGL LKKADPLEAK FLVRFVEGRL RVGIGDATVL 

       190        200        210        220        230        240 
DAMAIAFGGG QSASEIIERA YNLRADLGNI AKIIVEKGIE ALKTLKPQVG IPIRPMLAER 

       250        260        270        280        290        300 
LSNPEEILKK MGGNAIVDYK YDGERAQIHK KEDKIFIFSR RLENITSQYP DVVDYVSKYI 

       310        320        330        340        350        360 
EGKEFIIEGE IVAIDPESGE MRPFQELMHR KRKSDIYEAI KEYPVNVFLF DLMYYEDVDY 

       370        380        390        400        410        420 
TTKPLEARRK LLESIVKPND YVKIAHHIQA NNVEDLKSFF YRAISEGGEG VMVKAIGKDA 

       430        440        450        460        470        480 
IYQAGARGWL WIKLKRDYQS EMADTVDLVV VGGFYGKGKR GGKISSLLMA AYNPKTDSFE 

       490        500        510        520        530        540 
SVCKVASGFS DEQLDELQKK LMEIKRDVKH PRVNSKMEPD IWVEPVYVAE IIGSEITISP 

       550        560        570        580        590        600 
LHTCCQDVVE KDAGLSIRFP RFIRWRDDKS PEDATTTDEI LEMYNKQPKK KIESPAVDES 


V 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]"A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA."
Pascal J.M., Tsodikov O.V., Hura G.L., Song W., Cotner E.A., Classen S., Tomkinson A.E., Tainer J.A., Ellenberger T.
Mol. Cell 24:279-291(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEXES WITH ATP AND PCNA, PARTIAL PROTEIN SEQUENCE, SUBUNIT, MUTAGENESIS OF 110-PHE-LEU-111, COFACTOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006641 Genomic DNA. Translation: AAK40535.1.
PIRH90159.
RefSeqNP_341745.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HIVX-ray2.05A1-601[»]
2HIXX-ray2.87A1-601[»]
ProteinModelPortalQ980T8.
SMRQ980T8. Positions 1-590.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273057.SSO0189.

Proteomic databases

PRIDEQ980T8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK40535; AAK40535; SSO0189.
GeneID1455345.
KEGGsso:SSO0189.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAYIARFVV.
ProtClustDBPRK01109.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-178-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ980T8.

Entry information

Entry nameDNLI_SULSO
AccessionPrimary (citable) accession number: Q980T8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references