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Q980T8

- DNLI_SULSO

UniProt

Q980T8 - DNLI_SULSO

Protein

DNA ligase

Gene

lig

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Interaction with PCNA enhances ligase activity. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Divalent metal cations.1 Publication

    Enzyme regulationi

    Ligase activity stimulated by PCNA heterotrimer.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei258 – 2581ATP
    Active sitei260 – 2601N6-AMP-lysine intermediate
    Binding sitei265 – 2651ATP
    Binding sitei280 – 2801ATP
    Binding sitei310 – 3101ATPBy similarity
    Binding sitei350 – 3501ATPBy similarity
    Binding sitei427 – 4271ATP
    Binding sitei433 – 4331ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: RefGenome
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation Source: RefGenome
    4. DNA ligation involved in DNA repair Source: InterPro
    5. DNA recombination Source: UniProtKB-HAMAP
    6. lagging strand elongation Source: RefGenome
    7. nucleotide-excision repair Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-178-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase (EC:6.5.1.1)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
    Gene namesi
    Name:lig
    Ordered Locus Names:SSO0189
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: RefGenome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1 – 3030Missing: No interaction with PCNA3, no stimulation by PCNA heterotrimer. Add
    BLAST
    Mutagenesisi110 – 1112FL → AA: Impairs interaction with PCNA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 601601DNA ligasePRO_0000059618Add
    BLAST

    Proteomic databases

    PRIDEiQ980T8.

    Interactioni

    Subunit structurei

    Interacts with the PCNA heterotrimer, probably via subunit PCNA3.2 Publications

    Protein-protein interaction databases

    STRINGi273057.SSO0189.

    Structurei

    Secondary structure

    1
    601
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1512
    Helixi19 – 3113
    Helixi35 – 373
    Helixi38 – 447
    Turni45 – 473
    Helixi52 – 543
    Helixi63 – 7412
    Helixi78 – 8811
    Helixi91 – 10010
    Helixi121 – 13313
    Helixi139 – 15315
    Helixi156 – 16611
    Helixi176 – 18712
    Beta strandi188 – 1903
    Helixi191 – 1933
    Helixi194 – 20310
    Helixi207 – 21711
    Helixi219 – 2224
    Beta strandi237 – 2404
    Helixi244 – 2507
    Turni251 – 2533
    Beta strandi255 – 2606
    Beta strandi262 – 27110
    Beta strandi274 – 2785
    Helixi286 – 2883
    Helixi290 – 29910
    Beta strandi303 – 31412
    Turni316 – 3183
    Helixi325 – 3328
    Helixi336 – 3427
    Beta strandi345 – 35511
    Helixi365 – 37511
    Beta strandi380 – 3845
    Beta strandi387 – 3926
    Helixi393 – 40513
    Beta strandi410 – 4145
    Beta strandi427 – 4359
    Helixi436 – 4394
    Beta strandi440 – 4423
    Beta strandi444 – 45512
    Helixi458 – 4603
    Beta strandi463 – 47311
    Turni474 – 4774
    Beta strandi478 – 4858
    Helixi491 – 50212
    Beta strandi505 – 5084
    Beta strandi520 – 5234
    Beta strandi528 – 53912
    Turni544 – 5485
    Beta strandi554 – 5596
    Beta strandi561 – 5655
    Helixi571 – 5733
    Helixi577 – 58610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HIVX-ray2.05A1-601[»]
    2HIXX-ray2.87A1-601[»]
    ProteinModelPortaliQ980T8.
    SMRiQ980T8. Positions 1-590.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ980T8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiCTDIGEL.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q980T8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKTIIDK VVYIIQGKLW    50
    PDFLGYPELG IGEKFLIKAI SIATNTDENS VENLYKTIGD LGEVARRLKS 100
    KQQSTGILGF LGTTSKESLT VDEVYSTLSK VALTTGEGSR DLKIRLLAGL 150
    LKKADPLEAK FLVRFVEGRL RVGIGDATVL DAMAIAFGGG QSASEIIERA 200
    YNLRADLGNI AKIIVEKGIE ALKTLKPQVG IPIRPMLAER LSNPEEILKK 250
    MGGNAIVDYK YDGERAQIHK KEDKIFIFSR RLENITSQYP DVVDYVSKYI 300
    EGKEFIIEGE IVAIDPESGE MRPFQELMHR KRKSDIYEAI KEYPVNVFLF 350
    DLMYYEDVDY TTKPLEARRK LLESIVKPND YVKIAHHIQA NNVEDLKSFF 400
    YRAISEGGEG VMVKAIGKDA IYQAGARGWL WIKLKRDYQS EMADTVDLVV 450
    VGGFYGKGKR GGKISSLLMA AYNPKTDSFE SVCKVASGFS DEQLDELQKK 500
    LMEIKRDVKH PRVNSKMEPD IWVEPVYVAE IIGSEITISP LHTCCQDVVE 550
    KDAGLSIRFP RFIRWRDDKS PEDATTTDEI LEMYNKQPKK KIESPAVDES 600
    V 601
    Length:601
    Mass (Da):67,733
    Last modified:October 1, 2001 - v1
    Checksum:iDA6814F4A6F0546E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK40535.1.
    PIRiH90159.
    RefSeqiNP_341745.1. NC_002754.1.
    WP_009990427.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK40535; AAK40535; SSO0189.
    GeneIDi1455345.
    KEGGisso:SSO0189.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK40535.1 .
    PIRi H90159.
    RefSeqi NP_341745.1. NC_002754.1.
    WP_009990427.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HIV X-ray 2.05 A 1-601 [» ]
    2HIX X-ray 2.87 A 1-601 [» ]
    ProteinModelPortali Q980T8.
    SMRi Q980T8. Positions 1-590.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273057.SSO0189.

    Proteomic databases

    PRIDEi Q980T8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK40535 ; AAK40535 ; SSO0189 .
    GeneIDi 1455345.
    KEGGi sso:SSO0189.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi CTDIGEL.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-178-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q980T8.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus."
      Dionne I., Nookala R.K., Jackson S.P., Doherty A.J., Bell S.D.
      Mol. Cell 11:275-282(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PCNA3, SUBUNIT, MUTAGENESIS OF 1-MET--LEU-30.
    3. "A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA."
      Pascal J.M., Tsodikov O.V., Hura G.L., Song W., Cotner E.A., Classen S., Tomkinson A.E., Tainer J.A., Ellenberger T.
      Mol. Cell 24:279-291(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEXES WITH ATP AND PCNA, PARTIAL PROTEIN SEQUENCE, SUBUNIT, MUTAGENESIS OF 110-PHE-LEU-111, COFACTOR.

    Entry informationi

    Entry nameiDNLI_SULSO
    AccessioniPrimary (citable) accession number: Q980T8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2002
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3