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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.1 Publication

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.By similarity

Enzyme regulationi

Allosterically activated by GTP. Inhibited by CTP and UMP in combination.1 Publication

Kineticsi

  1. KM=55 µM for 5-phospho-alpha-D-ribose 1-diphosphate (in the presence of 1 mM GTP)1 Publication
  2. KM=19 µM for 5-phospho-alpha-D-ribose 1-diphosphate (without GTP)1 Publication
  3. KM=27 µM for uracil (in the presence of 1 mM GTP)1 Publication
  4. KM=1.4 µM for uracil (without GTP)1 Publication
  1. Vmax=15.8 µmol/min/mg enzyme (in the presence of 1 mM GTP)1 Publication
  2. Vmax=0.92 µmol/min/mg enzyme (without GTP)1 Publication

pH dependencei

Optimum pH is 5.0-5.6 at 60 degrees Celsius.1 Publication

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371CTP
Binding sitei80 – 8015-phospho-alpha-D-ribose 1-diphosphate
Binding sitei105 – 10515-phospho-alpha-D-ribose 1-diphosphate
Binding sitei203 – 2031Uracil; via amide nitrogen
Binding sitei209 – 20915-phospho-alpha-D-ribose 1-diphosphate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 302CTP
Nucleotide bindingi30 – 345GTP
Nucleotide bindingi87 – 9610CTP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-221-MONOMER.
BRENDAi2.4.2.9. 6163.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferase (EC:2.4.2.9)
Alternative name(s):
UMP pyrophosphorylase
UPRTase
Gene namesi
Name:upp
Ordered Locus Names:SSO0231
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Uracil phosphoribosyltransferasePRO_0000120927Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi273057.SSO0231.

Structurei

Secondary structure

1
216
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi9 – 1911Combined sources
Helixi25 – 4420Combined sources
Beta strandi50 – 556Combined sources
Beta strandi61 – 666Combined sources
Helixi68 – 714Combined sources
Beta strandi72 – 798Combined sources
Turni80 – 823Combined sources
Helixi83 – 9210Combined sources
Beta strandi97 – 1059Combined sources
Beta strandi119 – 1257Combined sources
Turni131 – 1333Combined sources
Beta strandi135 – 1395Combined sources
Beta strandi141 – 1466Combined sources
Helixi147 – 15610Combined sources
Helixi157 – 1593Combined sources
Beta strandi162 – 1676Combined sources
Beta strandi169 – 1724Combined sources
Helixi173 – 18210Combined sources
Beta strandi186 – 19712Combined sources
Beta strandi203 – 2064Combined sources
Helixi210 – 2156Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VSTX-ray2.80A1-216[»]
1XTTX-ray1.80A/B/C/D1-216[»]
1XTUX-ray2.80A/B/C/D/E/F/G/H1-216[»]
1XTVX-ray2.60A/B/C/D/E/F/G/H1-216[»]
3G6WX-ray2.90A/B/C/D1-216[»]
ProteinModelPortaliQ980Q4.
SMRiQ980Q4. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ980Q4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 14895-phospho-alpha-D-ribose 1-diphosphate binding
Regioni208 – 2103Uracil binding

Sequence similaritiesi

Belongs to the UPRTase family.Curated

Phylogenomic databases

eggNOGiarCOG04128. Archaea.
COG0035. LUCA.
HOGENOMiHOG000262755.
InParanoidiQ980Q4.
KOiK00761.
OMAiVTVIDHP.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_A. Upp_A. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q980Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLYVIDKPI TLHILTQLRD KYTDQINFRK NLVRLGRILG YEISNTLDYE
60 70 80 90 100
IVEVETPLGV KTKGVDITDL NNIVIINILR AAVPLVEGLL KAFPKARQGV
110 120 130 140 150
IGASRVEVDG KEVPKDMDVY IYYKKIPDIR AKVDNVIIAD PMIATASTML
160 170 180 190 200
KVLEEVVKAN PKRIYIVSII SSEYGVNKIL SKYPFIYLFT VAIDPELNNK
210
GYILPGLGDA GDRAFG
Length:216
Mass (Da):24,150
Last modified:October 1, 2001 - v1
Checksum:i08F934237C3FD158
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40574.1.
PIRiG90164.
RefSeqiWP_009990487.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40574; AAK40574; SSO0231.
GeneIDi27426527.
KEGGisso:SSO0231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40574.1.
PIRiG90164.
RefSeqiWP_009990487.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VSTX-ray2.80A1-216[»]
1XTTX-ray1.80A/B/C/D1-216[»]
1XTUX-ray2.80A/B/C/D/E/F/G/H1-216[»]
1XTVX-ray2.60A/B/C/D/E/F/G/H1-216[»]
3G6WX-ray2.90A/B/C/D1-216[»]
ProteinModelPortaliQ980Q4.
SMRiQ980Q4. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40574; AAK40574; SSO0231.
GeneIDi27426527.
KEGGisso:SSO0231.

Phylogenomic databases

eggNOGiarCOG04128. Archaea.
COG0035. LUCA.
HOGENOMiHOG000262755.
InParanoidiQ980Q4.
KOiK00761.
OMAiVTVIDHP.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
BioCyciSSOL273057:GCH2-221-MONOMER.
BRENDAi2.4.2.9. 6163.

Miscellaneous databases

EvolutionaryTraceiQ980Q4.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_A. Upp_A. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPP_SULSO
AccessioniPrimary (citable) accession number: Q980Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.