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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.1 Publication

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.By similarity

Enzyme regulationi

Allosterically activated by GTP. Inhibited by CTP and UMP in combination.1 Publication

Kineticsi

  1. KM=55 µM for 5-phospho-alpha-D-ribose 1-diphosphate (in the presence of 1 mM GTP)1 Publication
  2. KM=19 µM for 5-phospho-alpha-D-ribose 1-diphosphate (without GTP)1 Publication
  3. KM=27 µM for uracil (in the presence of 1 mM GTP)1 Publication
  4. KM=1.4 µM for uracil (without GTP)1 Publication
  1. Vmax=15.8 µmol/min/mg enzyme (in the presence of 1 mM GTP)1 Publication
  2. Vmax=0.92 µmol/min/mg enzyme (without GTP)1 Publication

pH dependencei

Optimum pH is 5.0-5.6 at 60 degrees Celsius.1 Publication

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37CTP1
Binding sitei805-phospho-alpha-D-ribose 1-diphosphate1
Binding sitei1055-phospho-alpha-D-ribose 1-diphosphate1
Binding sitei203Uracil; via amide nitrogen1
Binding sitei2095-phospho-alpha-D-ribose 1-diphosphate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 30CTP2
Nucleotide bindingi30 – 34GTP5
Nucleotide bindingi87 – 96CTP10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.4.2.9. 6163.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferase (EC:2.4.2.9)
Alternative name(s):
UMP pyrophosphorylase
UPRTase
Gene namesi
Name:upp
Ordered Locus Names:SSO0231
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209271 – 216Uracil phosphoribosyltransferaseAdd BLAST216

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi273057.SSO0231.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi9 – 19Combined sources11
Helixi25 – 44Combined sources20
Beta strandi50 – 55Combined sources6
Beta strandi61 – 66Combined sources6
Helixi68 – 71Combined sources4
Beta strandi72 – 79Combined sources8
Turni80 – 82Combined sources3
Helixi83 – 92Combined sources10
Beta strandi97 – 105Combined sources9
Beta strandi119 – 125Combined sources7
Turni131 – 133Combined sources3
Beta strandi135 – 139Combined sources5
Beta strandi141 – 146Combined sources6
Helixi147 – 156Combined sources10
Helixi157 – 159Combined sources3
Beta strandi162 – 167Combined sources6
Beta strandi169 – 172Combined sources4
Helixi173 – 182Combined sources10
Beta strandi186 – 197Combined sources12
Beta strandi203 – 206Combined sources4
Helixi210 – 215Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VSTX-ray2.80A1-216[»]
1XTTX-ray1.80A/B/C/D1-216[»]
1XTUX-ray2.80A/B/C/D/E/F/G/H1-216[»]
1XTVX-ray2.60A/B/C/D/E/F/G/H1-216[»]
3G6WX-ray2.90A/B/C/D1-216[»]
ProteinModelPortaliQ980Q4.
SMRiQ980Q4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ980Q4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 1485-phospho-alpha-D-ribose 1-diphosphate binding9
Regioni208 – 210Uracil binding3

Sequence similaritiesi

Belongs to the UPRTase family.Curated

Phylogenomic databases

eggNOGiarCOG04128. Archaea.
COG0035. LUCA.
HOGENOMiHOG000262755.
InParanoidiQ980Q4.
KOiK00761.
OMAiVTVIDHP.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_A. Upp_A. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q980Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLYVIDKPI TLHILTQLRD KYTDQINFRK NLVRLGRILG YEISNTLDYE
60 70 80 90 100
IVEVETPLGV KTKGVDITDL NNIVIINILR AAVPLVEGLL KAFPKARQGV
110 120 130 140 150
IGASRVEVDG KEVPKDMDVY IYYKKIPDIR AKVDNVIIAD PMIATASTML
160 170 180 190 200
KVLEEVVKAN PKRIYIVSII SSEYGVNKIL SKYPFIYLFT VAIDPELNNK
210
GYILPGLGDA GDRAFG
Length:216
Mass (Da):24,150
Last modified:October 1, 2001 - v1
Checksum:i08F934237C3FD158
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40574.1.
PIRiG90164.
RefSeqiWP_009990487.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40574; AAK40574; SSO0231.
GeneIDi27426527.
KEGGisso:SSO0231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40574.1.
PIRiG90164.
RefSeqiWP_009990487.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VSTX-ray2.80A1-216[»]
1XTTX-ray1.80A/B/C/D1-216[»]
1XTUX-ray2.80A/B/C/D/E/F/G/H1-216[»]
1XTVX-ray2.60A/B/C/D/E/F/G/H1-216[»]
3G6WX-ray2.90A/B/C/D1-216[»]
ProteinModelPortaliQ980Q4.
SMRiQ980Q4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40574; AAK40574; SSO0231.
GeneIDi27426527.
KEGGisso:SSO0231.

Phylogenomic databases

eggNOGiarCOG04128. Archaea.
COG0035. LUCA.
HOGENOMiHOG000262755.
InParanoidiQ980Q4.
KOiK00761.
OMAiVTVIDHP.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
BRENDAi2.4.2.9. 6163.

Miscellaneous databases

EvolutionaryTraceiQ980Q4.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_A. Upp_A. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPP_SULSO
AccessioniPrimary (citable) accession number: Q980Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.