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Protein

ORC1-type DNA replication protein 1

Gene

cdc6-1

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of DNA replication. May play essential roles in origin recognition and cell cycle control of replication. Binds to DNA, with a preference for molecules that contain a bubble, a fork, or a tail. Inhibits the binding of the MCM helicase to the origin DNA and strongly inhibits its DNA helicase activity. Also regulates the DNA polymerase and the nuclease activities of PolB1. Stimulates the DNA-binding activity of Cdc6-3.UniRule annotation4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081ATPUniRule annotation
Binding sitei220 – 2201ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 715ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ORC1-type DNA replication protein 1UniRule annotation
Gene namesi
Name:cdc6-1
Ordered Locus Names:SSO0257
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397ORC1-type DNA replication protein 1PRO_0000151018Add
BLAST

Post-translational modificationi

Autophosphorylated in vitro.

Proteomic databases

PRIDEiQ980N4.

Interactioni

Subunit structurei

Monomer. Interacts with Cdc6-3, MCM and PolB1.5 Publications

Protein-protein interaction databases

DIPiDIP-48851N.
STRINGi273057.SSO0257.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 244Combined sources
Beta strandi25 – 273Combined sources
Helixi37 – 459Combined sources
Helixi48 – 525Combined sources
Beta strandi59 – 635Combined sources
Helixi69 – 8315Combined sources
Beta strandi89 – 946Combined sources
Helixi95 – 984Combined sources
Helixi101 – 1088Combined sources
Turni109 – 1124Combined sources
Beta strandi118 – 1203Combined sources
Helixi123 – 13513Combined sources
Beta strandi141 – 1466Combined sources
Helixi148 – 1536Combined sources
Helixi159 – 16810Combined sources
Beta strandi175 – 1828Combined sources
Helixi184 – 1896Combined sources
Helixi193 – 1964Combined sources
Turni197 – 2004Combined sources
Beta strandi201 – 2055Combined sources
Helixi210 – 22415Combined sources
Helixi232 – 24413Combined sources
Helixi249 – 26517Combined sources
Helixi273 – 29321Combined sources
Helixi297 – 30913Combined sources
Helixi319 – 33315Combined sources
Helixi340 – 35213Combined sources
Beta strandi355 – 3606Combined sources
Beta strandi370 – 3767Combined sources
Helixi379 – 38810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QBYX-ray3.35A15-397[»]
ProteinModelPortaliQ980N4.
SMRiQ980N4. Positions 17-389.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ980N4.

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC6/cdc18 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00467. Archaea.
COG1474. LUCA.
HOGENOMiHOG000230140.
InParanoidiQ980N4.
KOiK10725.
OMAiTGWPTDQ.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01407. ORC1_type_DNA_replic_protein.
InterProiIPR003593. AAA+_ATPase.
IPR015163. Cdc6_C.
IPR014277. Orc1/Cdc6_arc.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02928. TIGR02928. 1 hit.

Sequencei

Sequence statusi: Complete.

Q980N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIIDEVIS SFKTSSIFIN REYLLPDYIP DELPHREDQI RKIASILAPL
60 70 80 90 100
YREEKPNNIF IYGLTGTGKT AVVKFVLSKL HKKFLGKFKH VYINTRQIDT
110 120 130 140 150
PYRVLADLLE SLDVKVPFTG LSIAELYRRL VKAVRDYGSQ VVIVLDEIDA
160 170 180 190 200
FVKKYNDDIL YKLSRINSEV NKSKISFIGI TNDVKFVDLL DPRVKSSLSE
210 220 230 240 250
EEIIFPPYNA EELEDILTKR AQMAFKPGVL PDNVIKLCAA LAAREHGDAR
260 270 280 290 300
RALDLLRVSG EIAERMKDTK VKEEYVYMAK EEIERDRVRD IILTLPFHSK
310 320 330 340 350
LVLMAVVSIS SEENVVSTTG AVYETYLNIC KKLGVEAVTQ RRVSDIINEL
360 370 380 390
DMVGILTAKV VNRGRYGKTK EIGLAVDKNI IVRSLIESDS RFADLWS
Length:397
Mass (Da):45,167
Last modified:October 1, 2001 - v1
Checksum:iA2A32A355A3ADD74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40596.1.
PIRiE90167.
RefSeqiWP_009990521.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40596; AAK40596; SSO0257.
GeneIDi25403723.
KEGGisso:SSO0257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40596.1.
PIRiE90167.
RefSeqiWP_009990521.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QBYX-ray3.35A15-397[»]
ProteinModelPortaliQ980N4.
SMRiQ980N4. Positions 17-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48851N.
STRINGi273057.SSO0257.

Proteomic databases

PRIDEiQ980N4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40596; AAK40596; SSO0257.
GeneIDi25403723.
KEGGisso:SSO0257.

Phylogenomic databases

eggNOGiarCOG00467. Archaea.
COG1474. LUCA.
HOGENOMiHOG000230140.
InParanoidiQ980N4.
KOiK10725.
OMAiTGWPTDQ.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-244-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ980N4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01407. ORC1_type_DNA_replic_protein.
InterProiIPR003593. AAA+_ATPase.
IPR015163. Cdc6_C.
IPR014277. Orc1/Cdc6_arc.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02928. TIGR02928. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus."
    De Felice M., Esposito L., Rossi M., Pisani F.M.
    Extremophiles 10:61-70(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, SUBUNIT, INTERACTION WITH CDC6-3, AUTOPHOSPHORYLATION.
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. "Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2."
    Jiang P.X., Wang J., Feng Y., He Z.G.
    Biochem. Biophys. Res. Commun. 361:651-658(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MCM.
  4. "Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2."
    Wang J., Jiang P.X., Feng H., Feng Y., He Z.G.
    Biochem. Biophys. Res. Commun. 363:63-70(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC6-3.
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  5. "Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions."
    Zhang L., Zhang L., Liu Y., Yang S., Gao C., Gong H., Feng Y., He Z.G.
    Proc. Natl. Acad. Sci. U.S.A. 106:7792-7797(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POLB1.
  6. "Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex."
    Dueber E.L., Corn J.E., Bell S.D., Berger J.M.
    Science 317:1210-1213(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 15-397 IN COMPLEX WITH CDC6-3 AND DNA.

Entry informationi

Entry nameiCDC61_SULSO
AccessioniPrimary (citable) accession number: Q980N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 1, 2001
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.