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Q97ZY3

- ENDA_SULSO

UniProt

Q97ZY3 - ENDA_SULSO

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Protein

tRNA-splicing endonuclease

Gene
endA, SSO0439
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191 By similarity
Active sitei127 – 1271 By similarity
Active sitei158 – 1581 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-421-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:SSO0439
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 182182tRNA-splicing endonucleaseUniRule annotationPRO_0000109481Add
BLAST

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Protein-protein interaction databases

STRINGi273057.SSO0439.

Structurei

3D structure databases

ProteinModelPortaliQ97ZY3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000107823.
KOiK01170.
OMAiALKFGSH.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01833. EndA_short.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97ZY3-1 [UniParc]FASTAAdd to Basket

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MVKALLVGSK VLVPSIDESR YLYSNGFYGK PIGISKPKGP KDIVRPLELS    50
LIESVYLTKK GLINVVDKNG DLLEYKKLYE YSAMKINKFE ILYKVYEDLR 100
EKGFIVRSGV KYGADFAVYT LGPGLEHAPY VVIAVDIDEE ITPHELLSFG 150
RVSHSTKKRL VLALVDRKSE GIRYIMFKWV KM 182
Length:182
Mass (Da):20,710
Last modified:October 1, 2001 - v1
Checksum:iFC27F4A79BE12293
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK40764.1.
PIRiE90188.
RefSeqiNP_341974.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40764; AAK40764; SSO0439.
GeneIDi1455574.
KEGGisso:SSO0439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK40764.1 .
PIRi E90188.
RefSeqi NP_341974.1. NC_002754.1.

3D structure databases

ProteinModelPortali Q97ZY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273057.SSO0439.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK40764 ; AAK40764 ; SSO0439 .
GeneIDi 1455574.
KEGGi sso:SSO0439.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000107823.
KOi K01170.
OMAi ALKFGSH.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-421-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01833. EndA_short.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiENDA_SULSO
AccessioniPrimary (citable) accession number: Q97ZY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2001
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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