Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acylphosphatase

Gene

acyP

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An acylphosphate + H2O = a carboxylate + phosphate.

Kineticsi

  1. KM=0.36 mM for benzoylphosphate at 25 degrees Celsius1 Publication

    pH dependencei

    Optimum pH is 4.7-5.7.1 Publication

    Temperature dependencei

    Optimum temperature is 81 degrees Celsius. Thermostable up to 100.8 degrees Celsius. Poorly active at 25 degrees Celsius. Enzymatic activity is increased 4-fold by temperature increase from 25 to 45 degrees Celsius and more than 9-fold at 98 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei30 – 3011 Publication
    Active sitei48 – 4811 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-844-MONOMER.
    BRENDAi3.6.1.7. 6163.
    SABIO-RKQ97ZL0.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acylphosphatase (EC:3.6.1.7)
    Alternative name(s):
    Acylphosphate phosphohydrolase
    Gene namesi
    Name:acyP
    Ordered Locus Names:SSO0887
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001974 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 101101AcylphosphatasePRO_0000326870Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi273057.SSO0887.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 113Combined sources
    Beta strandi13 – 2311Combined sources
    Beta strandi25 – 284Combined sources
    Helixi29 – 3911Combined sources
    Beta strandi43 – 486Combined sources
    Beta strandi50 – 523Combined sources
    Beta strandi54 – 618Combined sources
    Helixi62 – 7211Combined sources
    Beta strandi80 – 9011Combined sources
    Beta strandi96 – 1016Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Y9ONMR-A1-101[»]
    2BJDX-ray1.27A/B1-101[»]
    2BJEX-ray1.90A/C/E/G1-101[»]
    4OIXX-ray1.55A1-101[»]
    4OJ1X-ray1.70A/B1-101[»]
    4OJ3X-ray2.20A/B1-101[»]
    4OJGX-ray1.38A/B1-101[»]
    4OJHX-ray1.60A/B1-101[»]
    DisProtiDP00513.
    ProteinModelPortaliQ97ZL0.
    SMRiQ97ZL0. Positions 1-101.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ97ZL0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 10187Acylphosphatase-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the acylphosphatase family.Curated
    Contains 1 acylphosphatase-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiarCOG01674. Archaea.
    COG1254. LUCA.
    HOGENOMiHOG000292686.
    InParanoidiQ97ZL0.
    KOiK01512.
    OMAiIEQEYAD.

    Family and domain databases

    InterProiIPR001792. Acylphosphatase-like_dom.
    IPR017968. Acylphosphatase_CS.
    [Graphical view]
    PfamiPF00708. Acylphosphatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF54975. SSF54975. 1 hit.
    PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
    PS00151. ACYLPHOSPHATASE_2. 1 hit.
    PS51160. ACYLPHOSPHATASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q97ZL0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKWSDTEVF EMLKRMYARV YGLVQGVGFR KFVQIHAIRL GIKGYAKNLP
    60 70 80 90 100
    DGSVEVVAEG YEEALSKLLE RIKQGPPAAE VEKVDYSFSE YKGEFEDFET

    Y
    Length:101
    Mass (Da):11,636
    Last modified:October 1, 2001 - v1
    Checksum:i9BC4BF537EA7B4FA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41170.1.
    PIRiC90239.

    Genome annotation databases

    EnsemblBacteriaiAAK41170; AAK41170; SSO0887.
    KEGGisso:SSO0887.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41170.1.
    PIRiC90239.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Y9ONMR-A1-101[»]
    2BJDX-ray1.27A/B1-101[»]
    2BJEX-ray1.90A/C/E/G1-101[»]
    4OIXX-ray1.55A1-101[»]
    4OJ1X-ray1.70A/B1-101[»]
    4OJ3X-ray2.20A/B1-101[»]
    4OJGX-ray1.38A/B1-101[»]
    4OJHX-ray1.60A/B1-101[»]
    DisProtiDP00513.
    ProteinModelPortaliQ97ZL0.
    SMRiQ97ZL0. Positions 1-101.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi273057.SSO0887.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAK41170; AAK41170; SSO0887.
    KEGGisso:SSO0887.

    Phylogenomic databases

    eggNOGiarCOG01674. Archaea.
    COG1254. LUCA.
    HOGENOMiHOG000292686.
    InParanoidiQ97ZL0.
    KOiK01512.
    OMAiIEQEYAD.

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-844-MONOMER.
    BRENDAi3.6.1.7. 6163.
    SABIO-RKQ97ZL0.

    Miscellaneous databases

    EvolutionaryTraceiQ97ZL0.

    Family and domain databases

    InterProiIPR001792. Acylphosphatase-like_dom.
    IPR017968. Acylphosphatase_CS.
    [Graphical view]
    PfamiPF00708. Acylphosphatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF54975. SSF54975. 1 hit.
    PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
    PS00151. ACYLPHOSPHATASE_2. 1 hit.
    PS51160. ACYLPHOSPHATASE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "Structure, conformational stability, and enzymatic properties of acylphosphatase from the hyperthermophile Sulfolobus solfataricus."
      Corazza A., Rosano C., Pagano K., Alverdi V., Esposito G., Capanni C., Bemporad F., Plakoutsi G., Stefani M., Chiti F., Zuccotti S., Bolognesi M., Viglino P.
      Proteins 62:64-79(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.27 ANGSTROMS), STRUCTURE BY NMR, BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION BY MASS SPECTROMETRY, ACTIVE SITE.

    Entry informationi

    Entry nameiACYP_SULSO
    AccessioniPrimary (citable) accession number: Q97ZL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 1, 2001
    Last modified: February 17, 2016
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.