Q97ZG3 (RIFK_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	SSO0955

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Riboflavin kinase HAMAP MF_01285		PRO_0000322079
Regions
	Nucleotide binding	54 – 59	6	CDP By similarity
	Nucleotide binding	155 – 158	4	CDP By similarity
Sites
	Metal binding	83	1	Magnesium By similarity
	Metal binding	85	1	Magnesium By similarity
	Binding site	142	1	FMN By similarity
	Binding site	150	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97ZG3 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: AAB11E7C38199473	FASTA	175	19,586
	10 20 30 40 50 60 MEDDKLIVRI ISKEGEIIRL TEEGERFLSS CLSSLKDAIL SLHSIEIKGN IVSGLGEGKI 70 80 90 100 110 120 FLSMEYYKSQ INKIMGFDPF PGTLNIVIYD KASLENRLLL DSSPSLMVPE YKQKDRVLGA 130 140 150 160 170 VKLYPAAINK LTPAAVVIPL RTTHPKSVIE IISPFHLREK LNLKDGDEVT IEVYV

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK41229.1.
PIR	F90246.
RefSeq	NP_342439.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q97ZG3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455196.
GenomeReviews	Gene locus SSO0955 in contig AE006641_GR.
KEGG	sso:SSO0955.
NMPDR	fig|273057.1.peg.862.
Phylogenomic databases
HOGENOM	HBG553349.
OMA	PYEGTLN.
ProtClustDB	CLSK802937.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0955-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_SULSO

Accession

Primary (citable) accession number: Q97ZG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2001
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program