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Protein

Uridylate kinase

Gene

pyrH

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP, although much less efficiently.1 Publication

Catalytic activityi

ATP + UMP = ADP + UDP.

Enzyme regulationi

Unlike most bacteria, is not activated by GTP. UTP acts as a competitive inhibitor against both substrates. High concentration of UMP abolishes the inhibition of UTP at low ATP concentrations, indicating that UTP binds to the acceptor site (UMP site).1 Publication

Kineticsi

  1. KM=14 µM for UMP (at pH 7.2)1 Publication
  2. KM=81 µM for ATP (at pH 7.2)1 Publication
  1. Vmax=5 µmol/min/mg enzyme (at pH 5.5)1 Publication
  2. Vmax=45 µmol/min/mg enzyme (at pH 7.2)1 Publication
  3. Vmax=12 µmol/min/mg enzyme (at pH 8.5)1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44UMP; via amide nitrogen1 Publication1
Binding sitei45ATP; via amide nitrogen1
Binding sitei49ATP1
Binding sitei66UMP1 Publication1
Binding sitei140ATP1
Binding sitei141ATP1
Binding sitei146ATP; via amide nitrogen and carbonyl oxygen1
Binding sitei149ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 11ATP5
Nucleotide bindingi114 – 120UMP1 Publication7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.22. 6163.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinase (EC:2.7.4.22)
Short name:
UK
Alternative name(s):
Uridine monophosphate kinase
Short name:
UMP kinase
Short name:
UMPK
Gene namesi
Name:pyrH
Ordered Locus Names:SSO0976
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001439271 – 227Uridylate kinaseAdd BLAST227

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi273057.SSO0976.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi11 – 14Combined sources4
Helixi18 – 33Combined sources16
Beta strandi37 – 42Combined sources6
Helixi45 – 57Combined sources13
Helixi62 – 84Combined sources23
Helixi96 – 103Combined sources8
Beta strandi106 – 111Combined sources6
Helixi120 – 130Combined sources11
Beta strandi134 – 144Combined sources11
Beta strandi146 – 148Combined sources3
Turni150 – 152Combined sources3
Beta strandi153 – 155Combined sources3
Beta strandi160 – 163Combined sources4
Helixi164 – 171Combined sources8
Turni179 – 181Combined sources3
Helixi187 – 195Combined sources9
Beta strandi199 – 204Combined sources6
Helixi205 – 210Combined sources6
Helixi211 – 215Combined sources5
Beta strandi222 – 225Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J4JX-ray2.10A/B/C/D/E/F2-227[»]
2J4KX-ray2.20A/B/C/D/E/F2-227[»]
2J4LX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L2-227[»]
ProteinModelPortaliQ97ZE2.
SMRiQ97ZE2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97ZE2.

Family & Domainsi

Sequence similaritiesi

Belongs to the UMP kinase family.Curated

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
HOGENOMiHOG000047188.
InParanoidiQ97ZE2.
KOiK09903.
OMAiIRVIVMN.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97ZE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNIILKISG KFFDEDNVDN LIVLRQSIKE LADNGFRVGI VTGGGSTARR
60 70 80 90 100
YIKLAREIGI GEAYLDLLGI WASRLNAYLV MFSLQDLAYM HVPQSLEEFI
110 120 130 140 150
QDWSHGKVVV TGGFQPGQST AAVAALVAEA SSSKTLVVAT NVDGVYEKDP
160 170 180 190 200
RIYADVKLIP HLTTQDLRKI LEGSQSVQAG TYELLDPLAI KIVERSKIRV
210 220
IVMNYRKLNR IIDILKGEEV SSIIEPV
Length:227
Mass (Da):25,120
Last modified:October 1, 2001 - v1
Checksum:i25FB40F5D19B34F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41250.1.
PIRiC90249.

Genome annotation databases

EnsemblBacteriaiAAK41250; AAK41250; SSO0976.
KEGGisso:SSO0976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41250.1.
PIRiC90249.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J4JX-ray2.10A/B/C/D/E/F2-227[»]
2J4KX-ray2.20A/B/C/D/E/F2-227[»]
2J4LX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L2-227[»]
ProteinModelPortaliQ97ZE2.
SMRiQ97ZE2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0976.

Protocols and materials databases

DNASUi1455217.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41250; AAK41250; SSO0976.
KEGGisso:SSO0976.

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
HOGENOMiHOG000047188.
InParanoidiQ97ZE2.
KOiK09903.
OMAiIRVIVMN.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
BRENDAi2.7.4.22. 6163.

Miscellaneous databases

EvolutionaryTraceiQ97ZE2.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_SULSO
AccessioniPrimary (citable) accession number: Q97ZE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalysis proceeds by a sequential bi-bi reaction mechanism of random order.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.