Q97ZC5 (NADB_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aspartate oxidase
Short name=LASPO
EC=1.4.3.16

Alternative name(s):
Quinolinate synthase B

Gene names

Name:	nadB
Ordered Locus Names:	SSO0997

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.
Sequence caution	The sequence AAK41268.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC L-aspartate:fumarate oxidoreductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	L-aspartate oxidase		PRO_0000184411
Regions
	Nucleotide binding	3 – 17	15	FAD Potential
Sites
	Active site	211	1	By similarity
	Active site	229	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97ZC5 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: DABAE606463C353D	FASTA	472	52,645
	10 20 30 40 50 60 MIYIFGSGLA GLSAAISLHK SGYKVTIISK KINGGSSYWA KGGIAAAVGN DDSPELHKID 70 80 90 100 110 120 TLEVGDGLCD SKTVDYVTRE IRYVVSTVEK WGFKFDDDLR LEGGHSKRRI LHKTDDTGRE 130 140 150 160 170 180 ITNFLLNLAK KKGINLIEDK LLALKVKDGK VAGFITEKGG SFDAEKVVLA TGGYGYLFKF 190 200 210 220 230 240 TSNPSTNIGD GIAIAFKAGA LVSDTEFVQF HPTVTTFDGQ AYLLTETLRG EGAILVNERN 250 260 270 280 290 300 ERFVFKYDSR GELAPRDVLS RAIYDQYKKG HTVYIDLSPI EDFDRKFPIL SNYVKRYGKR 310 320 330 340 350 360 LQVFPGVHYT IGGIRVNTRG ESNIKGLYAI GEVTDTGLHG ANRLASNSLA EDLVYGVNLV 370 380 390 400 410 420 RYIDNWEGLS IDDVKEVIEV RLRNSSNRLS LEEIREYNWN YLGIVRNGEG LDKLVKIYES 430 440 450 460 470 NDTFNDNASL VSLLSAKGAL LRTESRGAHY REDYPNKSWE DGKRIYFMVS RN

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK41268.1. Different initiation.
PIR	E90251.
RefSeq	NP_342478.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q97ZC5.
SMR	Q97ZC5. Positions 1-467.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455235.
GenomeReviews	Gene locus SSO0997 in contig AE006641_GR.
KEGG	sso:SSO0997.
NMPDR	fig|273057.1.peg.901.
Phylogenomic databases
HOGENOM	HBG293998.
OMA	PTVYASC.
ProtClustDB	CLSK883692.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0997-MONOMER.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. [Graphical view]
Gene3D	G3DSA:1.20.58.100. Fum_Rdtase/Succ_DH_flav-like_C. 1 hit.
KO	K00278.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_SULSO

Accession

Primary (citable) accession number: Q97ZC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 30, 2002
Last modified:	December 14, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program