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Protein

DNA polymerase sliding clamp 2

Gene

pcn2

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clamp 2UniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homolog 2UniRule annotation
Short name:
PCNA2UniRule annotation
Gene namesi
Name:pcn2UniRule annotation
Synonyms:pcnA-2
Ordered Locus Names:SSO1047
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi146 – 1494LSDL → KLSK: Loss of interaction with PCNA3, no change with PCNA1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245DNA polymerase sliding clamp 2PRO_0000149216Add
BLAST

Interactioni

Subunit structurei

Forms homodimers with PCNA1, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. This subunit interacts with DNA polymerase I (dpo1) (PubMed:12535540). The heterotrimer also interacts with flap endonuclease 1, DNA ligase and XPF via the other subunits.8 Publications

Protein-protein interaction databases

STRINGi273057.SSO1047.

Structurei

Secondary structure

245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 66Combined sources
Helixi8 – 1912Combined sources
Beta strandi23 – 308Combined sources
Beta strandi33 – 397Combined sources
Beta strandi43 – 5210Combined sources
Helixi53 – 553Combined sources
Beta strandi56 – 649Combined sources
Beta strandi66 – 716Combined sources
Helixi72 – 798Combined sources
Beta strandi87 – 926Combined sources
Beta strandi94 – 11219Combined sources
Beta strandi129 – 1357Combined sources
Helixi136 – 14611Combined sources
Turni147 – 1493Combined sources
Beta strandi151 – 1588Combined sources
Beta strandi161 – 1677Combined sources
Beta strandi172 – 1776Combined sources
Beta strandi181 – 1833Combined sources
Beta strandi185 – 1895Combined sources
Beta strandi193 – 1975Combined sources
Helixi198 – 2036Combined sources
Helixi204 – 2096Combined sources
Beta strandi212 – 2187Combined sources
Beta strandi224 – 2296Combined sources
Helixi231 – 2333Combined sources
Beta strandi235 – 2406Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79B/Y1-245[»]
2HIKX-ray3.30B/M/Y1-245[»]
2IO4X-ray2.60B/D1-245[»]
2IX2X-ray2.20B1-245[»]
2IZOX-ray2.90B1-245[»]
2NTIX-ray2.50B/E/H1-245[»]
3FDSX-ray2.05D1-244[»]
ProteinModelPortaliQ97Z84.
SMRiQ97Z84. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97Z84.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
InParanoidiQ97Z84.
KOiK04802.
OMAiKCANSDD.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97Z84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKVIDAVS FSYILRTVGD FLSEANFIVT KEGIRVSGID PSRVVFLDIF
60 70 80 90 100
LPSSYFEGFE VSQEKEIIGF KLEDVNDILK RVLKDDTLIL SSNESKLTLT
110 120 130 140 150
FDGEFTRSFE LPLIQVESTQ PPSVNLEFPF KAQLLTITFA DIIDELSDLG
160 170 180 190 200
EVLNIHSKEN KLYFEVIGDL STAKVELSTD NGTLLEASGA DVSSSYGMEY
210 220 230 240
VANTTKMRRA SDSMELYFGS QIPLKLRFKL PQEGYGDFYI APRAD
Length:245
Mass (Da):27,436
Last modified:March 5, 2002 - v2
Checksum:i7A1B0506463DFE5E
GO

Sequence cautioni

The sequence AAK41309 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41309.1. Different initiation.
PIRiF90256.
RefSeqiWP_009989181.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41309; AAK41309; SSO1047.
GeneIDi27427360.
KEGGisso:SSO1047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41309.1. Different initiation.
PIRiF90256.
RefSeqiWP_009989181.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79B/Y1-245[»]
2HIKX-ray3.30B/M/Y1-245[»]
2IO4X-ray2.60B/D1-245[»]
2IX2X-ray2.20B1-245[»]
2IZOX-ray2.90B1-245[»]
2NTIX-ray2.50B/E/H1-245[»]
3FDSX-ray2.05D1-244[»]
ProteinModelPortaliQ97Z84.
SMRiQ97Z84. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO1047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41309; AAK41309; SSO1047.
GeneIDi27427360.
KEGGisso:SSO1047.

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
InParanoidiQ97Z84.
KOiK04802.
OMAiKCANSDD.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-992-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ97Z84.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA2_SULSO
AccessioniPrimary (citable) accession number: Q97Z84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: September 7, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.