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Protein

DNA polymerase sliding clamp 2

Gene

pcn2

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clamp 2UniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homolog 2UniRule annotation
Short name:
PCNA2UniRule annotation
Gene namesi
Name:pcn2UniRule annotation
Synonyms:pcnA-2
Ordered Locus Names:SSO1047
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi146 – 149LSDL → KLSK: Loss of interaction with PCNA3, no change with PCNA1. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492161 – 245DNA polymerase sliding clamp 2Add BLAST245

Interactioni

Subunit structurei

Forms homodimers with PCNA1, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. This subunit interacts with DNA polymerase I (dpo1) (PubMed:12535540). The heterotrimer also interacts with flap endonuclease 1, DNA ligase and XPF via the other subunits.8 Publications

Protein-protein interaction databases

STRINGi273057.SSO1047.

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 6Combined sources6
Helixi8 – 19Combined sources12
Beta strandi23 – 30Combined sources8
Beta strandi33 – 39Combined sources7
Beta strandi43 – 52Combined sources10
Helixi53 – 55Combined sources3
Beta strandi56 – 64Combined sources9
Beta strandi66 – 71Combined sources6
Helixi72 – 79Combined sources8
Beta strandi87 – 92Combined sources6
Beta strandi94 – 112Combined sources19
Beta strandi129 – 135Combined sources7
Helixi136 – 146Combined sources11
Turni147 – 149Combined sources3
Beta strandi151 – 158Combined sources8
Beta strandi161 – 167Combined sources7
Beta strandi172 – 177Combined sources6
Beta strandi181 – 183Combined sources3
Beta strandi185 – 189Combined sources5
Beta strandi193 – 197Combined sources5
Helixi198 – 203Combined sources6
Helixi204 – 209Combined sources6
Beta strandi212 – 218Combined sources7
Beta strandi224 – 229Combined sources6
Helixi231 – 233Combined sources3
Beta strandi235 – 240Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79B/Y1-245[»]
2HIKX-ray3.30B/M/Y1-245[»]
2IO4X-ray2.60B/D1-245[»]
2IX2X-ray2.20B1-245[»]
2IZOX-ray2.90B1-245[»]
2NTIX-ray2.50B/E/H1-245[»]
3FDSX-ray2.05D1-244[»]
ProteinModelPortaliQ97Z84.
SMRiQ97Z84.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97Z84.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
InParanoidiQ97Z84.
KOiK04802.
OMAiKCANSDD.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97Z84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKVIDAVS FSYILRTVGD FLSEANFIVT KEGIRVSGID PSRVVFLDIF
60 70 80 90 100
LPSSYFEGFE VSQEKEIIGF KLEDVNDILK RVLKDDTLIL SSNESKLTLT
110 120 130 140 150
FDGEFTRSFE LPLIQVESTQ PPSVNLEFPF KAQLLTITFA DIIDELSDLG
160 170 180 190 200
EVLNIHSKEN KLYFEVIGDL STAKVELSTD NGTLLEASGA DVSSSYGMEY
210 220 230 240
VANTTKMRRA SDSMELYFGS QIPLKLRFKL PQEGYGDFYI APRAD
Length:245
Mass (Da):27,436
Last modified:March 5, 2002 - v2
Checksum:i7A1B0506463DFE5E
GO

Sequence cautioni

The sequence AAK41309 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41309.1. Different initiation.
PIRiF90256.
RefSeqiWP_009989181.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41309; AAK41309; SSO1047.
GeneIDi27427360.
KEGGisso:SSO1047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41309.1. Different initiation.
PIRiF90256.
RefSeqiWP_009989181.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79B/Y1-245[»]
2HIKX-ray3.30B/M/Y1-245[»]
2IO4X-ray2.60B/D1-245[»]
2IX2X-ray2.20B1-245[»]
2IZOX-ray2.90B1-245[»]
2NTIX-ray2.50B/E/H1-245[»]
3FDSX-ray2.05D1-244[»]
ProteinModelPortaliQ97Z84.
SMRiQ97Z84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO1047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41309; AAK41309; SSO1047.
GeneIDi27427360.
KEGGisso:SSO1047.

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
InParanoidiQ97Z84.
KOiK04802.
OMAiKCANSDD.

Miscellaneous databases

EvolutionaryTraceiQ97Z84.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA2_SULSO
AccessioniPrimary (citable) accession number: Q97Z84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.