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Protein

DNA primase small subunit PriS

Gene

priS

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.UniRule annotation1 Publication

Miscellaneous

The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:16273105).1 Publication

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101UniRule annotation1
Active sitei103UniRule annotation1
Metal bindingi116Zinc1 Publication1
Metal bindingi119Zinc1 Publication1
Metal bindingi128Zinc1 Publication1
Metal bindingi131Zinc1 Publication1
Active sitei235UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandMagnesium, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.7.B16 6163

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase small subunit PriSUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:priSUniRule annotation
Synonyms:priA
Ordered Locus Names:SSO1048
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164F → E: Strong decrease in interaction with PriL. 1 Publication1
Mutagenesisi165G → I: Partial destabilization of PriS-PriL complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467541 – 330DNA primase small subunit PriSAdd BLAST330

Proteomic databases

PRIDEiQ97Z83

Interactioni

Subunit structurei

Heterodimer of a small subunit (PriS) and a large subunit (PriL).UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
priLQ9UWW15EBI-8081502,EBI-8081454

Protein-protein interaction databases

DIPiDIP-42889N
IntActiQ97Z83, 2 interactors
MINTiQ97Z83
STRINGi273057.SSO1048

Structurei

Secondary structure

1330
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 24Combined sources12
Helixi33 – 35Combined sources3
Beta strandi38 – 42Combined sources5
Beta strandi49 – 52Combined sources4
Helixi57 – 66Combined sources10
Beta strandi70 – 82Combined sources13
Helixi88 – 91Combined sources4
Beta strandi93 – 96Combined sources4
Beta strandi98 – 103Combined sources6
Turni104 – 108Combined sources5
Beta strandi112 – 115Combined sources4
Turni117 – 119Combined sources3
Beta strandi125 – 127Combined sources3
Turni129 – 131Combined sources3
Beta strandi136 – 139Combined sources4
Helixi143 – 162Combined sources20
Beta strandi169 – 172Combined sources4
Beta strandi174 – 182Combined sources9
Turni187 – 190Combined sources4
Helixi193 – 203Combined sources11
Helixi220 – 226Combined sources7
Helixi236 – 240Combined sources5
Beta strandi245 – 247Combined sources3
Turni254 – 256Combined sources3
Beta strandi262 – 264Combined sources3
Helixi266 – 268Combined sources3
Helixi273 – 275Combined sources3
Beta strandi280 – 288Combined sources9
Beta strandi290 – 294Combined sources5
Beta strandi297 – 301Combined sources5
Beta strandi307 – 310Combined sources4
Helixi311 – 319Combined sources9
Beta strandi322 – 328Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZT2X-ray3.33A/C1-330[»]
5OF3X-ray2.91A/D1-330[»]
5OFNX-ray3.00A1-330[»]
ProteinModelPortaliQ97Z83
SMRiQ97Z83
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97Z83

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic-type primase small subunit family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04110 Archaea
COG1467 LUCA
HOGENOMiHOG000223198
InParanoidiQ97Z83
KOiK02683
OMAiGYHVHVR
OrthoDBiPOG093Z04TM

Family and domain databases

CDDicd04860 AE_Prim_S, 1 hit
HAMAPiMF_00700 DNA_primase_sml_arc, 1 hit
InterProiView protein in InterPro
IPR002755 DNA_primase_S
IPR014052 DNA_primase_ssu_euk/arc
IPR023639 DNA_primase_ssu_PriS
PANTHERiPTHR10536 PTHR10536, 2 hits
PfamiView protein in Pfam
PF01896 DNA_primase_S, 1 hit

Sequencei

Sequence statusi: Complete.

Q97Z83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTFTLHQGQ TNLIKSFFRN YYLNAELELP KDMELREFAL QPFGSDTYVR
60 70 80 90 100
HLSFSSSEEL RDYLVNRNLP LHLFYSSARY QLPSARNMEE KAWMGSDLLF
110 120 130 140 150
DIDADHLCKL RSIRFCPVCG NAVVSEKCER DNVETLEYVE MTSECIKRGL
160 170 180 190 200
EQTRNLVEIL EDDFGLKPKV YFSGNRGFHV QVDCYGNCAL LDSDERKEIA
210 220 230 240 250
EYVMGIGVPG YPGGSENAPG WVGRKNRGIN GVTIDEQVTI DVKRLIRIPN
260 270 280 290 300
SLHGKSGLIV KRVPNLDDFE FNETLSPFTG YTIFLPYITI ETEVLGSIIK
310 320 330
LNRGIPIKIK SSIGIYLHLR NLGEVKAYVR
Length:330
Mass (Da):37,598
Last modified:October 1, 2001 - v1
Checksum:iE9B3C556E619B66E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA Translation: AAK41310.1
PIRiG90256
RefSeqiWP_009989180.1, NC_002754.1

Genome annotation databases

EnsemblBacteriaiAAK41310; AAK41310; SSO1048
GeneIDi27427361
KEGGisso:SSO1048
PATRICifig|273057.12.peg.1042

Similar proteinsi

Entry informationi

Entry nameiPRIS_SULSO
AccessioniPrimary (citable) accession number: Q97Z83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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