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Protein

Holliday junction resolvase Hje

Gene

hje

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.3 Publications

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+2 PublicationsNote: Binds 1 Mg2+ ion per subunit.2 Publications

Kineticsi

kcat is 4.8 min(-1) and 10 min (-1) at 5 mM and 15 mM MgCl2 respectively.

  1. KM=73 nM for 4-way junction DNA at 5 mM MgCl22 Publications
  2. KM=66 nM for 4-way junction DNA at 15 mM MgCl22 Publications

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius. Could be higher with a more thermally stable substrate.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi10Magnesium or manganeseSequence analysis1
    Metal bindingi39Magnesium or manganeseSequence analysis1
    Metal bindingi52Magnesium or manganeseSequence analysis1
    Sitei54Transition state stabilizerSequence analysis1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.22.4. 6163.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Holliday junction resolvase Hje (EC:3.1.22.4)
    Short name:
    Hje
    Gene namesi
    Name:hje
    Ordered Locus Names:SSO1176
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001974 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi30S → A, C or T: 100- to 1000-fold reduction in kcat. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004291591 – 135Holliday junction resolvase HjeAdd BLAST135

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi273057.SSO1176.

    Structurei

    Secondary structure

    1135
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi7 – 19Combined sources13
    Beta strandi23 – 26Combined sources4
    Beta strandi32 – 35Combined sources4
    Beta strandi39 – 44Combined sources6
    Beta strandi47 – 63Combined sources17
    Helixi65 – 76Combined sources12
    Beta strandi78 – 90Combined sources13
    Helixi91 – 93Combined sources3
    Beta strandi95 – 99Combined sources5
    Beta strandi102 – 105Combined sources4
    Beta strandi107 – 110Combined sources4
    Turni111 – 113Combined sources3
    Beta strandi114 – 116Combined sources3
    Helixi117 – 128Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OB8X-ray1.80A/B1-135[»]
    1OB9X-ray2.00A1-135[»]
    ProteinModelPortaliQ97YX6.
    SMRiQ97YX6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ97YX6.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiarCOG00919. Archaea.
    COG1591. LUCA.
    HOGENOMiHOG000270708.
    InParanoidiQ97YX6.
    KOiK03552.
    OMAiFLAMFPM.

    Family and domain databases

    CDDicd00523. archeal_HJR. 1 hit.
    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR002732. Hjc.
    IPR014428. Hjc_arc.
    IPR011335. Restrct_endonuc-II-like.
    IPR011856. tRNA_endonuc-like_dom.
    [Graphical view]
    PfamiPF01870. Hjc. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
    ProDomiPD015874. PD015874. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q97YX6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNRDIGKNAE RELVSILRGE GFNAVRIPTS NSSPNPLPDI FATKGNTLLS
    60 70 80 90 100
    IECKSTWENK VKVKEHQVRK LLDFLSMFTM KGVPLIAIKF KQVHEWRVLV
    110 120 130
    PEKAEDIIVT IDNSIPIEDL FKILEKRIEE KILTP
    Length:135
    Mass (Da):15,452
    Last modified:October 1, 2001 - v1
    Checksum:i13036E9997223BC2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41424.1.
    PIRiA90271.
    RefSeqiWP_009991563.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41424; AAK41424; SSO1176.
    GeneIDi27427480.
    KEGGisso:SSO1176.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41424.1.
    PIRiA90271.
    RefSeqiWP_009991563.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OB8X-ray1.80A/B1-135[»]
    1OB9X-ray2.00A1-135[»]
    ProteinModelPortaliQ97YX6.
    SMRiQ97YX6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi273057.SSO1176.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAK41424; AAK41424; SSO1176.
    GeneIDi27427480.
    KEGGisso:SSO1176.

    Phylogenomic databases

    eggNOGiarCOG00919. Archaea.
    COG1591. LUCA.
    HOGENOMiHOG000270708.
    InParanoidiQ97YX6.
    KOiK03552.
    OMAiFLAMFPM.

    Enzyme and pathway databases

    BRENDAi3.1.22.4. 6163.

    Miscellaneous databases

    EvolutionaryTraceiQ97YX6.

    Family and domain databases

    CDDicd00523. archeal_HJR. 1 hit.
    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR002732. Hjc.
    IPR014428. Hjc_arc.
    IPR011335. Restrct_endonuc-II-like.
    IPR011856. tRNA_endonuc-like_dom.
    [Graphical view]
    PfamiPF01870. Hjc. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
    ProDomiPD015874. PD015874. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52980. SSF52980. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHJE_SULSO
    AccessioniPrimary (citable) accession number: Q97YX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: October 1, 2001
    Last modified: November 2, 2016
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A second Holliday junction resolving enzyme, Hjc, with different substrate specificity exists in this organism.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.