Reviewed, UniProtKB/Swiss-Prot Q97YJ9 (ILVC2_SULSO)

Names and origin

Protein names

Recommended name:
    Putative ketol-acid reductoisomerase 2
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 2
    Alpha-keto-beta-hydroxylacil reductoisomerase 2

Gene names

Name:	ilvC2
Synonyms:	ilvC-2
Ordered Locus Names:	SSO1322

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.
Caution	Ser-107 is present instead of the conserved His which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Putative ketol-acid reductoisomerase 2 HAMAP MF_00435		PRO_0000151401

Sequences

	Sequence		Length	Mass (Da)
	Q97YJ9-1 [UniParc]. Last modified August 29, 2001. Version 1. Checksum: D37083F704B95CA0	FASTA	333	37,185
	10 20 30 40 50 60 MDKTVLDANL DPLKGKTIGV IGYGNQGRVQ ATIMRENGLN VIVGNVKDKY YELAKKEGFE 70 80 90 100 110 120 VYEIDEAVRR SDVALLLIPD EVMKEVYEKK IAPVLQGKKE FVLDFASGYN VAFGLIRPPK 130 140 150 160 170 180 SVDTIMVAPR MVGEGIMDLH KQGKGYPVLL GVKQDASGKA WDYAKAIAKG IGAIPGGIAV 190 200 210 220 230 240 ISSFEEEALL DLMSEHTWVP ILFGAIKACY DIAVKEYGVS PEAALLEFYA SGELAEIARL 250 260 270 280 290 300 IAEEGIFNQM VHHSTTSQYG TLTRMFKYYD VVRRIVENEA KYIWDGSFAK EWSLEQQAGY 310 320 330 PVFYRLWELA TQSEMAKAEK ELYKLLGRKV KND

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006641 Genomic DNA. Translation: AAK41560.1.
PIR	A99288.
RefSeq	NP_342770.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1454339.
GenomeReviews	Gene locus SSO1322 in contig AE006641_GR.
KEGG	sso:SSO1322.
NMPDR	fig|273057.1.peg.1193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97YJ9.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1322-MON.
BRENDA	1.1.1.86. 2070.
Family and domain databases
HAMAP	MF_00435. Divergent sequence. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC2_SULSO

Accession

Primary (citable) accession number: Q97YJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)