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Protein

CRISPR-associated endoribonuclease Cas2 1

Gene

cas21

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Involved in the integration of spacer DNA into the CRISPR cassette (By similarity). Functions as a ssRNA-specific endoribonuclease, producing a 5'-phosphomonoester and a 3'-hydroxy. Does not process pre-crRNA in the manner expected if it were the CRISPR-processing endoribonuclease. Prefers U-rich substrates and often cuts between adjacent U residues in regions predicted to be single-stranded. RNAs as short as 10 residues can serve as substrate.By similarity1 Publication

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 8.5-9.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium; catalyticSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-1324-MONOMER.
BRENDAi3.1.26.12. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated endoribonuclease Cas2 1 (EC:3.1.-.-)
Alternative name(s):
CAS2 endoribonuclease
Gene namesi
Name:cas21
Ordered Locus Names:SSO1404
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91Y → A: Considerable loss of RNase activity. 1 Publication
Mutagenesisi10 – 101D → A: Loss of RNase activity. 1 Publication
Mutagenesisi17 – 171R → A: Considerable loss of RNase activity. 1 Publication
Mutagenesisi19 – 191R → A: Considerable loss of RNase activity. 1 Publication
Mutagenesisi31 – 311R → A: Loss of RNase activity. 1 Publication
Mutagenesisi37 – 371F → A: Loss of RNase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 101101CRISPR-associated endoribonuclease Cas2 1PRO_0000416951Add
BLAST

Interactioni

Subunit structurei

Forms a heterotetramer with a Cas1 homodimer (By similarity). Homodimer.By similarity1 Publication

Protein-protein interaction databases

STRINGi273057.SSO1404.

Structurei

Secondary structure

1
101
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 119Combined sources
Helixi14 – 2613Combined sources
Beta strandi30 – 334Combined sources
Beta strandi36 – 416Combined sources
Helixi43 – 5614Combined sources
Beta strandi68 – 758Combined sources
Helixi77 – 815Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I8EX-ray1.59A1-101[»]
2IVYX-ray1.40A2-101[»]
ProteinModelPortaliQ97YC2.
SMRiQ97YC2. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97YC2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04194. Archaea.
COG1343. LUCA.
HOGENOMiHOG000226099.
InParanoidiQ97YC2.
KOiK09951.
OMAiGYSGSER.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
HAMAPiMF_01471. Cas2.
InterProiIPR021127. CRISPR_associated_Cas2.
IPR000640. EFG_V.
IPR019199. Virulence_VapD/CRISPR_Cas2.
[Graphical view]
PfamiPF09827. CRISPR_Cas2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01573. cas2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97YC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLYLIFYD ITDDNLRNRV AEFLKKKGLD RIQYSVFMGD LNSSRLKDVE
60 70 80 90 100
AGLKIIGNRK KLQEDERFFI LIVPITENQF RERIVIGYSG SEREEKSNVV

W
Length:101
Mass (Da):11,936
Last modified:October 1, 2001 - v1
Checksum:iA7C338AD76202E17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41639.1.
PIRiH90297.

Genome annotation databases

EnsemblBacteriaiAAK41639; AAK41639; SSO1404.
KEGGisso:SSO1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41639.1.
PIRiH90297.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I8EX-ray1.59A1-101[»]
2IVYX-ray1.40A2-101[»]
ProteinModelPortaliQ97YC2.
SMRiQ97YC2. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO1404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41639; AAK41639; SSO1404.
KEGGisso:SSO1404.

Phylogenomic databases

eggNOGiarCOG04194. Archaea.
COG1343. LUCA.
HOGENOMiHOG000226099.
InParanoidiQ97YC2.
KOiK09951.
OMAiGYSGSER.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-1324-MONOMER.
BRENDAi3.1.26.12. 6163.

Miscellaneous databases

EvolutionaryTraceiQ97YC2.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
HAMAPiMF_01471. Cas2.
InterProiIPR021127. CRISPR_associated_Cas2.
IPR000640. EFG_V.
IPR019199. Virulence_VapD/CRISPR_Cas2.
[Graphical view]
PfamiPF09827. CRISPR_Cas2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01573. cas2. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats."
    Beloglazova N., Brown G., Zimmerman M.D., Proudfoot M., Makarova K.S., Kudritska M., Kochinyan S., Wang S., Chruszcz M., Minor W., Koonin E.V., Edwards A.M., Savchenko A., Yakunin A.F.
    J. Biol. Chem. 283:20361-20371(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS), FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE, SUBSTRATE SPECIFICITY, PH-DEPENDENCE, COFACTOR, SUBUNIT, MUTAGENESIS OF TYR-9; ASP-10; ARG-17; ARG-19; ARG-31 AND PHE-37.
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 2-101.

Entry informationi

Entry nameiCAS2A_SULSO
AccessioniPrimary (citable) accession number: Q97YC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2001
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.