Reviewed, UniProtKB/Swiss-Prot Q97X13 (ILVC3_SULSO)

Names and origin

Protein names

Recommended name:
    Putative ketol-acid reductoisomerase 3
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 3
    Alpha-keto-beta-hydroxylacil reductoisomerase 3

Gene names

Name:	ilvC3
Synonyms:	ilvC-3
Ordered Locus Names:	SSO1942

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.
Caution	Ser-107 is present instead of the conserved His which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Putative ketol-acid reductoisomerase 3		PRO_0000151402

Sequences

	Sequence		Length	Mass (Da)
	Q97X13-1 [UniParc]. Last modified August 29, 2001. Version 1. Checksum: E4861E33EAC6AF22	FASTA	332	37,071
	10 20 30 40 50 60 MDKTVLDASL EPLKGKTIAV IGYGNQGRVQ ANIMRENGLN VIIGNVKDRY YDLAIKEGFE 70 80 90 100 110 120 VYDIGEAVKK ADVAFLLIPD EIMKEIYEKK IAPILEGKKE FVLDFASGYN VAFGLIRPPK 130 140 150 160 170 180 NVDTVMVAPR MVGEGIMDLH KQGKGYPVLL GVKQDASGKA WDYAKAIAKG IGAIPGGIAV 190 200 210 220 230 240 ISSFEEEALL DLMSEHTWVP ILFGAIKACF DVAVKEYGVS PEAALLEFYA SGELVEIARL 250 260 270 280 290 300 IAEEGIFNQM VHHSTTSQYG TLTRMFKYYD LVKEIVKDEA KYIWDGSFAK EWTLEQQAGY 310 320 330 PVFYRLWELA VQSEMAKAEK ELYKILRRKV SD

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006802 Genomic DNA. Translation: AAK42136.1.
PIR	A90360.
RefSeq	NP_343346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1453463.
GenomeReviews	Gene locus SSO1942 in contig AE006641_GR.
KEGG	sso:SSO1942.
NMPDR	fig|273057.1.peg.1769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97X13.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1942-MON.
Family and domain databases
HAMAP	MF_00435. Divergent sequence. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC3_SULSO

Accession

Primary (citable) accession number: Q97X13

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)