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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2032-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:SSO2172
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278NH(3)-dependent NAD(+) synthetasePRO_0000152235Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273057.SSO2172.

Structurei

3D structure databases

ProteinModelPortaliQ97WN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00069. Archaea.
COG0171. LUCA.
HOGENOMiHOG000238069.
InParanoidiQ97WN9.
KOiK01916.
OMAiTGLYVKW.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97WN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEYIRKSLT IDCEAVTNYI VERIREYLEF SNKKGGVIGV SGGVDSAVTA
60 70 80 90 100
TLLAKATDNF FILLMPSSST PKIDLDDSFE MIKFLNAQNK YKLINIDEIV
110 120 130 140 150
KSFSNKIETE NKYIIGNIKA RVRMIILYAY AQMLDYLVVG TGDKSELLLG
160 170 180 190 200
YFTKYGDGGV DVLPIGDLYK TQVRMLGKCL GLPERIVTKP SSPALWEGQT
210 220 230 240 250
AEGELGIDYE TIDSILYLRF DEMRSEDEIV KMLGIPIDIV KKVDRLVKIS
260 270
QHKRLPPEIF RLSGRAINSD WRFPRRWA
Length:278
Mass (Da):31,593
Last modified:November 15, 2002 - v2
Checksum:i8D3E6061E4E66C3A
GO

Sequence cautioni

The sequence AAK42347.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42347.1. Different initiation.
PIRiD90386.
RefSeqiWP_009992131.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42347; AAK42347; SSO2172.
GeneIDi25405363.
KEGGisso:SSO2172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42347.1. Different initiation.
PIRiD90386.
RefSeqiWP_009992131.1. NC_002754.1.

3D structure databases

ProteinModelPortaliQ97WN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42347; AAK42347; SSO2172.
GeneIDi25405363.
KEGGisso:SSO2172.

Phylogenomic databases

eggNOGiarCOG00069. Archaea.
COG0171. LUCA.
HOGENOMiHOG000238069.
InParanoidiQ97WN9.
KOiK01916.
OMAiTGLYVKW.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciSSOL273057:GCH2-2032-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiNADE_SULSO
AccessioniPrimary (citable) accession number: Q97WN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: June 8, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.