Q97WN9 (NADE_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SSO2172

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.
Sequence caution	The sequence AAK42347.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152235
Regions
	Nucleotide binding	39 – 46	8	ATP By similarity
Sites
	Active site	41	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97WN9 [UniParc]. Last modified November 15, 2002. Version 2. Checksum: 8D3E6061E4E66C3A	FASTA	278	31,593
	10 20 30 40 50 60 MHEYIRKSLT IDCEAVTNYI VERIREYLEF SNKKGGVIGV SGGVDSAVTA TLLAKATDNF 70 80 90 100 110 120 FILLMPSSST PKIDLDDSFE MIKFLNAQNK YKLINIDEIV KSFSNKIETE NKYIIGNIKA 130 140 150 160 170 180 RVRMIILYAY AQMLDYLVVG TGDKSELLLG YFTKYGDGGV DVLPIGDLYK TQVRMLGKCL 190 200 210 220 230 240 GLPERIVTKP SSPALWEGQT AEGELGIDYE TIDSILYLRF DEMRSEDEIV KMLGIPIDIV 250 260 270 KKVDRLVKIS QHKRLPPEIF RLSGRAINSD WRFPRRWA

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK42347.1. Different initiation.
PIR	D90386.
RefSeq	NP_343557.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q97WN9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1453674.
GenomeReviews	Gene locus SSO2172 in contig AE006641_GR.
KEGG	sso:SSO2172.
NMPDR	fig|273057.1.peg.1980.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO2172-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_SULSO

Accession

Primary (citable) accession number: Q97WN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	November 16, 2011
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program