Q97WG4 (CAPPA_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	SSO2256

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Ref.2
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. Ref.2
Cofactor	Magnesium. Mg2+ can not be replaced by Mn2+. Ref.2
Enzyme regulation	Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A. Ref.2
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the PEPCase type 2 family.
Biophysicochemical properties	Kinetic parameters: KM=0.09 mM for phosphoenolpyruvate Ref.2 pH dependence: Optimum pH is 8.0. Temperature dependence: Optimum temperature is 85 degrees Celsius.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from direct assay Ref.2. Source: UniProtKB oxaloacetate metabolic process Inferred from direct assay Ref.2. Source: UniProtKB tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from direct assay Ref.2. Source: UniProtKB phosphoenolpyruvate carboxylase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904		PRO_0000309615

Sequences

	Sequence		Length	Mass (Da)
	Q97WG4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 90E0795BB2EC2105	FASTA	511	58,772
	10 20 30 40 50 60 MRIIPRTMST QHPDNAKVPE WAKSEVIEGE DEVKEAFLAY SMYGVHEVMW DAEGKDVDTH 70 80 90 100 110 120 VVRKLLSNYP DYFREHILGK DLFLTYRLPN PKVEGADRKV FAETMESIPI TYDLAEKFYG 130 140 150 160 170 180 NGITIPVFEV ILPMTTSSLE IISVARYYEK AVANEDELEL YDGVKVKDLV GEIYPKVIEV 190 200 210 220 230 240 IPLVEDRDSL QNINNIVEGY YKVIKPKYMR VFLARSDPAM NYGMITAVLS VKIALSELYK 250 260 270 280 290 300 LSESLNFEIY PIIGVGSLPF RGHLSPENYE KVLEEYKGVY TYTIQSAFKY DYDYDKVKSA 310 320 330 340 350 360 ISSINNSRIS PARILEKYEE DVLRKITILY TERYQPIIES LANAINDVSV LLPRRRARKL 370 380 390 400 410 420 HIGLFGYSRS AGKVSLPRAI SFVGSLYSIG IPPELIGISS LSNLDEKEWD IFKQNYVNFK 430 440 450 460 470 480 HDLQTAARFL NWESFKLIKD IWKISEDTIA KIKEDIDYAE SVIGIKLGGI DYDSRKHILM 490 500 510 SSLFLLSFKE KILQESKKYL YEMALIRRSL G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism." Ettema T.J., Makarova K.S., Jellema G.L., Gierman H.J., Koonin E.V., Huynen M.A., de Vos W.M., van der Oost J. J. Bacteriol. 186:7754-7762(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK42423.1.
PIR	H90395.
RefSeq	NP_343633.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q97WG4.
ModBase	Search...
Protein-protein interaction databases
STRING	273057.SSO2256.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK42423; AAK42423; SSO2256.
GeneID	1453750.
KEGG	sso:SSO2256.
Phylogenomic databases
eggNOG	COG1892.
HOGENOM	HOG000038601.
KO	K01595.
OMA	MCTQHPD.
ProtClustDB	PRK13655.
Family and domain databases
HAMAP	MF_01904. PEPcase_type2.
InterPro	IPR007566. PEP_COase_arc-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PIRSF	PIRSF006677. UCP006677. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_SULSO

Accession

Primary (citable) accession number: Q97WG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program