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Q97WG4

- CAPPA_SULSO

UniProt

Q97WG4 - CAPPA_SULSO

Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.1 PublicationUniRule annotation

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.1 PublicationUniRule annotation

    Cofactori

    Magnesium. Mg2+ can not be replaced by Mn2+.1 PublicationUniRule annotation

    Enzyme regulationi

    Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A.1 Publication

    Kineticsi

    1. KM=0.09 mM for phosphoenolpyruvate1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB
    2. phosphoenolpyruvate carboxylase activity Source: UniProtKB

    GO - Biological processi

    1. carbon fixation Source: UniProtKB
    2. oxaloacetate metabolic process Source: UniProtKB
    3. tricarboxylic acid cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-2108-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
    Short name:
    PEPCUniRule annotation
    Short name:
    PEPCaseUniRule annotation
    Gene namesi
    Name:ppcAUniRule annotation
    Ordered Locus Names:SSO2256
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511Phosphoenolpyruvate carboxylasePRO_0000309615Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273057.SSO2256.

    Structurei

    3D structure databases

    ProteinModelPortaliQ97WG4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1892.
    HOGENOMiHOG000038601.
    KOiK01595.
    OMAiDEYMPDY.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q97WG4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIIPRTMST QHPDNAKVPE WAKSEVIEGE DEVKEAFLAY SMYGVHEVMW    50
    DAEGKDVDTH VVRKLLSNYP DYFREHILGK DLFLTYRLPN PKVEGADRKV 100
    FAETMESIPI TYDLAEKFYG NGITIPVFEV ILPMTTSSLE IISVARYYEK 150
    AVANEDELEL YDGVKVKDLV GEIYPKVIEV IPLVEDRDSL QNINNIVEGY 200
    YKVIKPKYMR VFLARSDPAM NYGMITAVLS VKIALSELYK LSESLNFEIY 250
    PIIGVGSLPF RGHLSPENYE KVLEEYKGVY TYTIQSAFKY DYDYDKVKSA 300
    ISSINNSRIS PARILEKYEE DVLRKITILY TERYQPIIES LANAINDVSV 350
    LLPRRRARKL HIGLFGYSRS AGKVSLPRAI SFVGSLYSIG IPPELIGISS 400
    LSNLDEKEWD IFKQNYVNFK HDLQTAARFL NWESFKLIKD IWKISEDTIA 450
    KIKEDIDYAE SVIGIKLGGI DYDSRKHILM SSLFLLSFKE KILQESKKYL 500
    YEMALIRRSL G 511
    Length:511
    Mass (Da):58,772
    Last modified:October 1, 2001 - v1
    Checksum:i90E0795BB2EC2105
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK42423.1.
    PIRiH90395.
    RefSeqiNP_343633.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK42423; AAK42423; SSO2256.
    GeneIDi1453750.
    KEGGisso:SSO2256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK42423.1 .
    PIRi H90395.
    RefSeqi NP_343633.1. NC_002754.1.

    3D structure databases

    ProteinModelPortali Q97WG4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273057.SSO2256.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK42423 ; AAK42423 ; SSO2256 .
    GeneIDi 1453750.
    KEGGi sso:SSO2256.

    Phylogenomic databases

    eggNOGi COG1892.
    HOGENOMi HOG000038601.
    KOi K01595.
    OMAi DEYMPDY.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-2108-MONOMER.

    Family and domain databases

    HAMAPi MF_01904. PEPcase_type2.
    InterProi IPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF14010. PEPcase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006677. UCP006677. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR02751. PEPCase_arch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism."
      Ettema T.J., Makarova K.S., Jellema G.L., Gierman H.J., Koonin E.V., Huynen M.A., de Vos W.M., van der Oost J.
      J. Bacteriol. 186:7754-7762(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

    Entry informationi

    Entry nameiCAPPA_SULSO
    AccessioniPrimary (citable) accession number: Q97WG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3