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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotation1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 PublicationNote: Mg(2+) cannot be replaced by Mn2+.UniRule annotation1 Publication

Enzyme regulationi

Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A.1 Publication

Kineticsi

  1. KM=0.09 mM for phosphoenolpyruvate1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 85 degrees Celsius.1 Publication

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB

GO - Biological processi

  1. carbon fixation Source: UniProtKB
  2. oxaloacetate metabolic process Source: UniProtKB
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2108-MONOMER.
BRENDAi4.1.1.31. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcAUniRule annotation
Ordered Locus Names:SSO2256
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Phosphoenolpyruvate carboxylasePRO_0000309615Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi273057.SSO2256.

Structurei

3D structure databases

ProteinModelPortaliQ97WG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1892.
HOGENOMiHOG000038601.
InParanoidiQ97WG4.
KOiK01595.
OMAiQSSFKYD.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97WG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIPRTMST QHPDNAKVPE WAKSEVIEGE DEVKEAFLAY SMYGVHEVMW
60 70 80 90 100
DAEGKDVDTH VVRKLLSNYP DYFREHILGK DLFLTYRLPN PKVEGADRKV
110 120 130 140 150
FAETMESIPI TYDLAEKFYG NGITIPVFEV ILPMTTSSLE IISVARYYEK
160 170 180 190 200
AVANEDELEL YDGVKVKDLV GEIYPKVIEV IPLVEDRDSL QNINNIVEGY
210 220 230 240 250
YKVIKPKYMR VFLARSDPAM NYGMITAVLS VKIALSELYK LSESLNFEIY
260 270 280 290 300
PIIGVGSLPF RGHLSPENYE KVLEEYKGVY TYTIQSAFKY DYDYDKVKSA
310 320 330 340 350
ISSINNSRIS PARILEKYEE DVLRKITILY TERYQPIIES LANAINDVSV
360 370 380 390 400
LLPRRRARKL HIGLFGYSRS AGKVSLPRAI SFVGSLYSIG IPPELIGISS
410 420 430 440 450
LSNLDEKEWD IFKQNYVNFK HDLQTAARFL NWESFKLIKD IWKISEDTIA
460 470 480 490 500
KIKEDIDYAE SVIGIKLGGI DYDSRKHILM SSLFLLSFKE KILQESKKYL
510
YEMALIRRSL G
Length:511
Mass (Da):58,772
Last modified:September 30, 2001 - v1
Checksum:i90E0795BB2EC2105
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42423.1.
PIRiH90395.
RefSeqiNP_343633.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42423; AAK42423; SSO2256.
GeneIDi1453750.
KEGGisso:SSO2256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42423.1.
PIRiH90395.
RefSeqiNP_343633.1. NC_002754.1.

3D structure databases

ProteinModelPortaliQ97WG4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42423; AAK42423; SSO2256.
GeneIDi1453750.
KEGGisso:SSO2256.

Phylogenomic databases

eggNOGiCOG1892.
HOGENOMiHOG000038601.
InParanoidiQ97WG4.
KOiK01595.
OMAiQSSFKYD.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2108-MONOMER.
BRENDAi4.1.1.31. 6163.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism."
    Ettema T.J., Makarova K.S., Jellema G.L., Gierman H.J., Koonin E.V., Huynen M.A., de Vos W.M., van der Oost J.
    J. Bacteriol. 186:7754-7762(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiCAPPA_SULSO
AccessioniPrimary (citable) accession number: Q97WG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 12, 2007
Last sequence update: September 30, 2001
Last modified: March 31, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.