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Q97W94

- MTAP_SULSO

UniProt

Q97W94 - MTAP_SULSO

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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.1 PublicationUniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Kineticsi

  1. KM=0.7 mM for S-methyl-5'-thioadenosine1 Publication
  2. KM=270 µM for adenosine1 Publication

Temperature dependencei

Optimum temperature is 120 degrees Celsius. Highly thermostable.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161PhosphateUniRule annotation
Sitei171 – 1711Important for substrate specificityUniRule annotation
Binding sitei190 – 1901Substrate; via amide nitrogenUniRule annotation
Binding sitei191 – 1911PhosphateUniRule annotation
Sitei225 – 2251Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2180-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPIIUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:SSO2343
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi259 – 2591C → S: Reduces thermostability of the enzyme; when associated with S-261. 1 Publication
Mutagenesisi261 – 2611C → S: Reduces thermostability of the enzyme; when associated with S-259. 1 Publication
Mutagenesisi262 – 2621C → S: Reduces thermostability of the enzyme. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270S-methyl-5'-thioadenosine phosphorylasePRO_0000415109Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi138 ↔ 2051 Publication
Disulfide bondi200 ↔ 2621 Publication
Disulfide bondi259 ↔ 2611 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.2 PublicationsUniRule annotation

Protein-protein interaction databases

STRINGi273057.SSO2343.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 146Combined sources
Beta strandi25 – 306Combined sources
Beta strandi43 – 486Combined sources
Beta strandi51 – 577Combined sources
Turni58 – 603Combined sources
Helixi61 – 633Combined sources
Helixi67 – 693Combined sources
Helixi72 – 8110Combined sources
Beta strandi86 – 9712Combined sources
Beta strandi110 – 1145Combined sources
Beta strandi125 – 1273Combined sources
Helixi139 – 15214Combined sources
Beta strandi156 – 1583Combined sources
Beta strandi161 – 1655Combined sources
Helixi173 – 1819Combined sources
Beta strandi187 – 1915Combined sources
Helixi192 – 20110Combined sources
Beta strandi205 – 21410Combined sources
Beta strandi218 – 2214Combined sources
Helixi225 – 23410Combined sources
Helixi236 – 24914Combined sources
Helixi256 – 2583Combined sources
Turni260 – 2634Combined sources
Helixi266 – 2683Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A8YX-ray1.45A/B/C/D/E/F/G/H/I/J/K/L1-270[»]
3T94X-ray1.45A/B/C/D/E/F1-270[»]
ProteinModelPortaliQ97W94.
SMRiQ97W94. Positions 1-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97W94.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 592Phosphate bindingUniRule annotation
Regioni91 – 922Phosphate bindingUniRule annotation
Regioni214 – 2163Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
InParanoidiQ97W94.
KOiK00772.
OMAiCEAQLCY.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97W94-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIEQNEKASI GIIGGSGLYD PGIFSESKEI KVYTPYGQPS DFITIGKIGN
60 70 80 90 100
KSVAFLPRHG RGHRIPPHKI NYRANIWALK ELGVRWVISV SAVGSLRMDY
110 120 130 140 150
KLGDFVIPDQ FIDMTKNREY SFFDGPVVAH VSMADPFCNS LRKLAIETAK
160 170 180 190 200
ELNIKTHESG TYICIEGPRF STRAESRTWR EVYKADIIGM TLVPEVNLAC
210 220 230 240 250
EAQMCYATIA MVTDYDVFAE IPVTAEEVTR VMAENTEKAK KLLYALIQKL
260 270
PEKPEEGSCS CCNSLKTALV
Length:270
Mass (Da):30,141
Last modified:October 1, 2001 - v1
Checksum:i682A166698935A88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42494.1.
PIRiG90404.
RefSeqiNP_343704.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42494; AAK42494; SSO2343.
GeneIDi1453821.
KEGGisso:SSO2343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42494.1 .
PIRi G90404.
RefSeqi NP_343704.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A8Y X-ray 1.45 A/B/C/D/E/F/G/H/I/J/K/L 1-270 [» ]
3T94 X-ray 1.45 A/B/C/D/E/F 1-270 [» ]
ProteinModelPortali Q97W94.
SMRi Q97W94. Positions 1-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273057.SSO2343.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK42494 ; AAK42494 ; SSO2343 .
GeneIDi 1453821.
KEGGi sso:SSO2343.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228986.
InParanoidi Q97W94.
KOi K00772.
OMAi CEAQLCY.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00873 .
BioCyci SSOL273057:GCH2-2180-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q97W94.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
PROSITEi PS01240. PNP_MTAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus."
    Cacciapuoti G., Forte S., Moretti M.A., Brio A., Zappia V., Porcelli M.
    FEBS J. 272:1886-1899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF CYS-259; CYS-261 AND CYS-262, SUBUNIT.
  3. "The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds."
    Zhang Y., Porcelli M., Cacciapuoti G., Ealick S.E.
    J. Mol. Biol. 357:252-262(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH SUBSTRATES, DISULFIDE BONDS.

Entry informationi

Entry nameiMTAP_SULSO
AccessioniPrimary (citable) accession number: Q97W94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 1, 2001
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3