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Q97W94

- MTAP_SULSO

UniProt

Q97W94 - MTAP_SULSO

Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.1 PublicationUniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Kineticsi

    1. KM=0.7 mM for S-methyl-5'-thioadenosine1 Publication
    2. KM=270 µM for adenosine1 Publication

    Temperature dependencei

    Optimum temperature is 120 degrees Celsius. Highly thermostable.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei16 – 161PhosphateUniRule annotation
    Sitei171 – 1711Important for substrate specificityUniRule annotation
    Binding sitei190 – 1901Substrate; via amide nitrogenUniRule annotation
    Binding sitei191 – 1911PhosphateUniRule annotation
    Sitei225 – 2251Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-2180-MONOMER.
    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Short name:
    MTAPIIUniRule annotation
    Gene namesi
    Name:mtnPUniRule annotation
    Ordered Locus Names:SSO2343
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi259 – 2591C → S: Reduces thermostability of the enzyme; when associated with S-261. 1 Publication
    Mutagenesisi261 – 2611C → S: Reduces thermostability of the enzyme; when associated with S-259. 1 Publication
    Mutagenesisi262 – 2621C → S: Reduces thermostability of the enzyme. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270S-methyl-5'-thioadenosine phosphorylasePRO_0000415109Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi138 ↔ 2051 Publication
    Disulfide bondi200 ↔ 2621 Publication
    Disulfide bondi259 ↔ 2611 Publication

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.2 PublicationsUniRule annotation

    Protein-protein interaction databases

    STRINGi273057.SSO2343.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 146
    Beta strandi25 – 306
    Beta strandi43 – 486
    Beta strandi51 – 577
    Turni58 – 603
    Helixi61 – 633
    Helixi67 – 693
    Helixi72 – 8110
    Beta strandi86 – 9712
    Beta strandi110 – 1145
    Beta strandi125 – 1273
    Helixi139 – 15214
    Beta strandi156 – 1583
    Beta strandi161 – 1655
    Helixi173 – 1819
    Beta strandi187 – 1915
    Helixi192 – 20110
    Beta strandi205 – 21410
    Beta strandi218 – 2214
    Helixi225 – 23410
    Helixi236 – 24914
    Helixi256 – 2583
    Turni260 – 2634
    Helixi266 – 2683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2A8YX-ray1.45A/B/C/D/E/F/G/H/I/J/K/L1-270[»]
    3T94X-ray1.45A/B/C/D/E/F1-270[»]
    ProteinModelPortaliQ97W94.
    SMRiQ97W94. Positions 1-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ97W94.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni58 – 592Phosphate bindingUniRule annotation
    Regioni91 – 922Phosphate bindingUniRule annotation
    Regioni214 – 2163Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228986.
    KOiK00772.
    OMAiCEAQLCY.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q97W94-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEQNEKASI GIIGGSGLYD PGIFSESKEI KVYTPYGQPS DFITIGKIGN    50
    KSVAFLPRHG RGHRIPPHKI NYRANIWALK ELGVRWVISV SAVGSLRMDY 100
    KLGDFVIPDQ FIDMTKNREY SFFDGPVVAH VSMADPFCNS LRKLAIETAK 150
    ELNIKTHESG TYICIEGPRF STRAESRTWR EVYKADIIGM TLVPEVNLAC 200
    EAQMCYATIA MVTDYDVFAE IPVTAEEVTR VMAENTEKAK KLLYALIQKL 250
    PEKPEEGSCS CCNSLKTALV 270
    Length:270
    Mass (Da):30,141
    Last modified:October 1, 2001 - v1
    Checksum:i682A166698935A88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK42494.1.
    PIRiG90404.
    RefSeqiNP_343704.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK42494; AAK42494; SSO2343.
    GeneIDi1453821.
    KEGGisso:SSO2343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK42494.1 .
    PIRi G90404.
    RefSeqi NP_343704.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2A8Y X-ray 1.45 A/B/C/D/E/F/G/H/I/J/K/L 1-270 [» ]
    3T94 X-ray 1.45 A/B/C/D/E/F 1-270 [» ]
    ProteinModelPortali Q97W94.
    SMRi Q97W94. Positions 1-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273057.SSO2343.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK42494 ; AAK42494 ; SSO2343 .
    GeneIDi 1453821.
    KEGGi sso:SSO2343.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228986.
    KOi K00772.
    OMAi CEAQLCY.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .
    BioCyci SSOL273057:GCH2-2180-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q97W94.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus."
      Cacciapuoti G., Forte S., Moretti M.A., Brio A., Zappia V., Porcelli M.
      FEBS J. 272:1886-1899(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF CYS-259; CYS-261 AND CYS-262, SUBUNIT.
    3. "The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds."
      Zhang Y., Porcelli M., Cacciapuoti G., Ealick S.E.
      J. Mol. Biol. 357:252-262(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH SUBSTRATES, DISULFIDE BONDS.

    Entry informationi

    Entry nameiMTAP_SULSO
    AccessioniPrimary (citable) accession number: Q97W94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3