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Protein

Hexaprenyl pyrophosphate synthase

Gene

gdS-2

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes consecutive E-type condensation of two isopentenyl pyrophosphate (IPP) molecules with an allylic substrate such as geranylgeranyl diphosphate (GGPP), farnesyl diphosphate (FPP) or geranyl diphosphate (GPP) to yield the medium-chain product trans-C30-hexaprenyl pyrophosphate (HexPP). GGPP is the physiological substrate.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate + 2 isopentenyl diphosphate = 2 diphosphate + all-trans-hexaprenyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

pH dependencei

Optimum pH is 6.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421Isopentenyl diphosphateBy similarity
Binding sitei45 – 451Isopentenyl diphosphateBy similarity
Binding sitei74 – 741Isopentenyl diphosphateBy similarity
Metal bindingi81 – 811Magnesium 1By similarity
Metal bindingi81 – 811Magnesium 2By similarity
Metal bindingi85 – 851Magnesium 1By similarity
Metal bindingi85 – 851Magnesium 2By similarity
Binding sitei91 – 911Isopentenyl diphosphateBy similarity
Metal bindingi200 – 2001Magnesium 3By similarity

GO - Molecular functioni

GO - Biological processi

  • isoprenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15679.
SSOL273057:GCH2-2182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexaprenyl pyrophosphate synthase (EC:2.5.1.82)
Short name:
HexPP
Alternative name(s):
Medium-chain HexPP
Gene namesi
Name:gdS-2
Ordered Locus Names:SSO2345
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi124 – 1241Y → A: Generates the same C30 product as the wild-type. 1 Publication
Mutagenesisi164 – 1641L → A: Generates only one C5-prenyl unit longer C35 product. 1 Publication
Mutagenesisi174 – 1741Y → A: Generates the same C30 product as the wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Hexaprenyl pyrophosphate synthasePRO_0000418599Add
BLAST

Interactioni

Subunit structurei

Heterodimer.2 Publications

Protein-protein interaction databases

STRINGi273057.SSO2345.

Structurei

Secondary structure

1
281
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2423Combined sources
Turni28 – 303Combined sources
Helixi31 – 388Combined sources
Helixi44 – 5512Combined sources
Helixi60 – 634Combined sources
Helixi64 – 8421Combined sources
Beta strandi89 – 946Combined sources
Helixi96 – 12429Combined sources
Helixi126 – 14520Combined sources
Turni146 – 1505Combined sources
Helixi153 – 1608Combined sources
Helixi162 – 17514Combined sources
Helixi179 – 1813Combined sources
Helixi182 – 20524Combined sources
Turni210 – 2123Combined sources
Helixi217 – 2204Combined sources
Helixi221 – 2244Combined sources
Turni225 – 2273Combined sources
Helixi229 – 24921Combined sources
Helixi255 – 2573Combined sources
Helixi258 – 2625Combined sources
Helixi264 – 27411Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AZJX-ray2.40A/B1-281[»]
2AZKX-ray2.70A/B1-281[»]
ProteinModelPortaliQ97W92.
SMRiQ97W92. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97W92.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiarCOG01727. Archaea.
COG0142. LUCA.
HOGENOMiHOG000232204.
InParanoidiQ97W92.
OMAiEMSKYIM.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97W92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIEFWLEA KATIDRLIEQ FLNSNRDWDL VDISSYILKD GKRFRGTLNM
60 70 80 90 100
FFTVALGGDI KDSYGGALAI EILHSASLAL DDIVDLDATR RGDKAAWVVY
110 120 130 140 150
GNRKVIFITN YLIPTALRII QTSYGDDALN TSIELWKDTS VGALRDMYDN
160 170 180 190 200
SDYIRTIELK TGSLFKLSTV LSAYASKHYN TKQQMLDVGK YLGIIYQVID
210 220 230 240 250
DFVDYKTKKV EEIDGSAKQL FKYYREGKLE EYVRSVYLEY KQKYDELISN
260 270 280
IPFQSKYLSE IRSLPEFLAN GLLKEANIDK I
Length:281
Mass (Da):32,275
Last modified:October 1, 2001 - v1
Checksum:iAD14ABD58DE76CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42496.1.
PIRiA99405.
RefSeqiWP_009989514.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42496; AAK42496; SSO2345.
GeneIDi27428655.
KEGGisso:SSO2345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42496.1.
PIRiA99405.
RefSeqiWP_009989514.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AZJX-ray2.40A/B1-281[»]
2AZKX-ray2.70A/B1-281[»]
ProteinModelPortaliQ97W92.
SMRiQ97W92. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42496; AAK42496; SSO2345.
GeneIDi27428655.
KEGGisso:SSO2345.

Phylogenomic databases

eggNOGiarCOG01727. Archaea.
COG0142. LUCA.
HOGENOMiHOG000232204.
InParanoidiQ97W92.
OMAiEMSKYIM.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15679.
SSOL273057:GCH2-2182-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ97W92.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEXPP_SULSO
AccessioniPrimary (citable) accession number: Q97W92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2001
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.