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Protein

Hexaprenyl pyrophosphate synthase

Gene

gdS-2

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes consecutive E-type condensation of two isopentenyl pyrophosphate (IPP) molecules with an allylic substrate such as geranylgeranyl diphosphate (GGPP), farnesyl diphosphate (FPP) or geranyl diphosphate (GPP) to yield the medium-chain product trans-C30-hexaprenyl pyrophosphate (HexPP). GGPP is the physiological substrate.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate + 2 isopentenyl diphosphate = 2 diphosphate + all-trans-hexaprenyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

pH dependencei

Optimum pH is 6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42Isopentenyl diphosphateBy similarity1
Binding sitei45Isopentenyl diphosphateBy similarity1
Binding sitei74Isopentenyl diphosphateBy similarity1
Metal bindingi81Magnesium 1By similarity1
Metal bindingi81Magnesium 2By similarity1
Metal bindingi85Magnesium 1By similarity1
Metal bindingi85Magnesium 2By similarity1
Binding sitei91Isopentenyl diphosphateBy similarity1
Metal bindingi200Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

  • isoprenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15679.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexaprenyl pyrophosphate synthase (EC:2.5.1.82)
Short name:
HexPP
Alternative name(s):
Medium-chain HexPP
Gene namesi
Name:gdS-2
Ordered Locus Names:SSO2345
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124Y → A: Generates the same C30 product as the wild-type. 1 Publication1
Mutagenesisi164L → A: Generates only one C5-prenyl unit longer C35 product. 1 Publication1
Mutagenesisi174Y → A: Generates the same C30 product as the wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004185991 – 281Hexaprenyl pyrophosphate synthaseAdd BLAST281

Interactioni

Subunit structurei

Heterodimer.2 Publications

Protein-protein interaction databases

STRINGi273057.SSO2345.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 24Combined sources23
Turni28 – 30Combined sources3
Helixi31 – 38Combined sources8
Helixi44 – 55Combined sources12
Helixi60 – 63Combined sources4
Helixi64 – 84Combined sources21
Beta strandi89 – 94Combined sources6
Helixi96 – 124Combined sources29
Helixi126 – 145Combined sources20
Turni146 – 150Combined sources5
Helixi153 – 160Combined sources8
Helixi162 – 175Combined sources14
Helixi179 – 181Combined sources3
Helixi182 – 205Combined sources24
Turni210 – 212Combined sources3
Helixi217 – 220Combined sources4
Helixi221 – 224Combined sources4
Turni225 – 227Combined sources3
Helixi229 – 249Combined sources21
Helixi255 – 257Combined sources3
Helixi258 – 262Combined sources5
Helixi264 – 274Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AZJX-ray2.40A/B1-281[»]
2AZKX-ray2.70A/B1-281[»]
ProteinModelPortaliQ97W92.
SMRiQ97W92.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97W92.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiarCOG01727. Archaea.
COG0142. LUCA.
HOGENOMiHOG000232204.
InParanoidiQ97W92.
OMAiEMSKYIM.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97W92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIEFWLEA KATIDRLIEQ FLNSNRDWDL VDISSYILKD GKRFRGTLNM
60 70 80 90 100
FFTVALGGDI KDSYGGALAI EILHSASLAL DDIVDLDATR RGDKAAWVVY
110 120 130 140 150
GNRKVIFITN YLIPTALRII QTSYGDDALN TSIELWKDTS VGALRDMYDN
160 170 180 190 200
SDYIRTIELK TGSLFKLSTV LSAYASKHYN TKQQMLDVGK YLGIIYQVID
210 220 230 240 250
DFVDYKTKKV EEIDGSAKQL FKYYREGKLE EYVRSVYLEY KQKYDELISN
260 270 280
IPFQSKYLSE IRSLPEFLAN GLLKEANIDK I
Length:281
Mass (Da):32,275
Last modified:October 1, 2001 - v1
Checksum:iAD14ABD58DE76CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42496.1.
PIRiA99405.
RefSeqiWP_009989514.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42496; AAK42496; SSO2345.
GeneIDi27428655.
KEGGisso:SSO2345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42496.1.
PIRiA99405.
RefSeqiWP_009989514.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AZJX-ray2.40A/B1-281[»]
2AZKX-ray2.70A/B1-281[»]
ProteinModelPortaliQ97W92.
SMRiQ97W92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42496; AAK42496; SSO2345.
GeneIDi27428655.
KEGGisso:SSO2345.

Phylogenomic databases

eggNOGiarCOG01727. Archaea.
COG0142. LUCA.
HOGENOMiHOG000232204.
InParanoidiQ97W92.
OMAiEMSKYIM.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15679.

Miscellaneous databases

EvolutionaryTraceiQ97W92.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEXPP_SULSO
AccessioniPrimary (citable) accession number: Q97W92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2001
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.