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Protein

Aryldialkylphosphatase

Gene

php

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a low paraoxonase activity. Also active, but with a lower activity, against other organo-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.1 Publication

Catalytic activityi

An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.1 Publication

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inactivated by EDTA and o-phenanthroline.1 Publication

Kineticsi

  1. KM=0.06 mM for paraoxon (at pH 8.0)1 Publication
  2. KM=0.205 mM for methyl-paraoxon (at pH 8.0)1 Publication

    pH dependencei

    Optimum pH is 7.0-9.0.1 Publication

    Temperature dependencei

    Thermostable.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi22Iron; via tele nitrogenCombined sources1 Publication1
    Metal bindingi24Iron; via tele nitrogenCombined sources1 Publication1
    Metal bindingi137Cobalt; via carbamate groupCombined sources1 Publication1
    Metal bindingi137Iron; via carbamate groupCombined sources1 Publication1
    Metal bindingi170Cobalt; via pros nitrogenCombined sources1 Publication1
    Metal bindingi199Cobalt; via tele nitrogenCombined sources1 Publication1
    Metal bindingi256IronCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandCobalt, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.1.25 6163
    3.1.1.81 6163
    3.1.8.1 6163

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aryldialkylphosphatase (EC:3.1.8.1)
    Alternative name(s):
    Paraoxonase
    Short name:
    SsoPox
    Phosphotriesterase-like lactonase
    Gene namesi
    Name:php
    Ordered Locus Names:SSO2522
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001974 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003886841 – 314AryldialkylphosphataseAdd BLAST314

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei137N6-carboxylysinePROSITE-ProRule annotationCombined sources1 Publication1

    Proteomic databases

    PRIDEiQ97VT7

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi273057.SSO2522

    Structurei

    Secondary structure

    1314
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 5Combined sources3
    Beta strandi8 – 11Combined sources4
    Helixi13 – 15Combined sources3
    Beta strandi18 – 22Combined sources5
    Helixi30 – 35Combined sources6
    Helixi37 – 39Combined sources3
    Helixi42 – 58Combined sources17
    Beta strandi63 – 66Combined sources4
    Turni71 – 73Combined sources3
    Helixi76 – 86Combined sources11
    Beta strandi89 – 92Combined sources4
    Beta strandi94 – 96Combined sources3
    Helixi104 – 106Combined sources3
    Helixi111 – 123Combined sources13
    Beta strandi126 – 130Combined sources5
    Beta strandi135 – 139Combined sources5
    Helixi147 – 163Combined sources17
    Beta strandi167 – 170Combined sources4
    Turni173 – 176Combined sources4
    Helixi177 – 187Combined sources11
    Helixi192 – 194Combined sources3
    Beta strandi195 – 197Combined sources3
    Helixi200 – 202Combined sources3
    Helixi206 – 214Combined sources9
    Beta strandi218 – 221Combined sources4
    Turni227 – 229Combined sources3
    Helixi232 – 244Combined sources13
    Helixi248 – 250Combined sources3
    Beta strandi251 – 253Combined sources3
    Beta strandi258 – 261Combined sources4
    Helixi264 – 266Combined sources3
    Helixi268 – 270Combined sources3
    Helixi271 – 274Combined sources4
    Beta strandi279 – 281Combined sources3
    Helixi282 – 285Combined sources4
    Helixi287 – 293Combined sources7
    Helixi298 – 305Combined sources8
    Helixi307 – 312Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2VC5X-ray2.60A/B/C/D1-314[»]
    2VC7X-ray2.05A/B/C/D1-314[»]
    3UF9X-ray2.68A/B/C/D1-314[»]
    4KERX-ray2.60A/B/C/D1-314[»]
    4KESX-ray2.10A/B/C/D1-314[»]
    4KETX-ray2.00A/B/C/D1-314[»]
    4KEUX-ray2.20A/B/C/D1-314[»]
    4KEVX-ray2.65A/B/C/D1-314[»]
    4KEZX-ray1.85A/B/C/D1-314[»]
    4KF1X-ray2.00A/B/C/D1-314[»]
    5VRIX-ray2.15A/B/C/D1-314[»]
    5VRKX-ray1.40A/B1-314[»]
    5VSAX-ray2.00A/B/C/D1-314[»]
    5W3UX-ray2.50A/B/C/D1-314[»]
    5W3WX-ray2.95A/B/C/D1-314[»]
    5W3ZX-ray2.55A/B/C/D1-314[»]
    ProteinModelPortaliQ97VT7
    SMRiQ97VT7
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ97VT7

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiarCOG07263 Archaea
    COG1735 LUCA
    HOGENOMiHOG000081700
    InParanoidiQ97VT7
    KOiK07048
    OMAiPFQIIRV
    OrthoDBiPOG093Z09MD

    Family and domain databases

    CDDicd00530 PTE, 1 hit
    InterProiView protein in InterPro
    IPR017947 AryldialkylPase_Zn-BS
    IPR032466 Metal_Hydrolase
    IPR001559 Phosphotriesterase
    PANTHERiPTHR10819 PTHR10819, 2 hits
    PfamiView protein in Pfam
    PF02126 PTE, 1 hit
    PIRSFiPIRSF016839 PhP, 1 hit
    SUPFAMiSSF51556 SSF51556, 1 hit
    PROSITEiView protein in PROSITE
    PS01322 PHOSPHOTRIESTERASE_1, 1 hit
    PS51347 PHOSPHOTRIESTERASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q97VT7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRIPLVGKDS IESKDIGFTL IHEHLRVFSE AVRQQWPHLY NEDEEFRNAV
    60 70 80 90 100
    NEVKRAMQFG VKTIVDPTVM GLGRDIRFME KVVKATGINL VAGTGIYIYI
    110 120 130 140 150
    DLPFYFLNRS IDEIADLFIH DIKEGIQGTL NKAGFVKIAA DEPGITKDVE
    160 170 180 190 200
    KVIRAAAIAN KETKVPIITH SNAHNNTGLE QQRILTEEGV DPGKILIGHL
    210 220 230 240 250
    GDTDNIDYIK KIADKGSFIG LDRYGLDLFL PVDKRNETTL RLIKDGYSDK
    260 270 280 290 300
    IMISHDYCCT IDWGTAKPEY KPKLAPRWSI TLIFEDTIPF LKRNGVNEEV
    310
    IATIFKENPK KFFS
    Length:314
    Mass (Da):35,565
    Last modified:October 1, 2001 - v1
    Checksum:iB5A28F80C3CB1A31
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006641 Genomic DNA Translation: AAK42653.1
    AY775568 Genomic DNA Translation: AAW47234.1
    PIRiF90424
    RefSeqiWP_009988477.1, NC_002754.1

    Genome annotation databases

    EnsemblBacteriaiAAK42653; AAK42653; SSO2522
    GeneIDi27428822
    KEGGisso:SSO2522
    PATRICifig|273057.12.peg.2599

    Similar proteinsi

    Entry informationi

    Entry nameiPHP_SULSO
    AccessioniPrimary (citable) accession number: Q97VT7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: October 1, 2001
    Last modified: May 23, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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