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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871SubstrateUniRule annotation
Binding sitei211 – 2111Substrate; via amide nitrogenUniRule annotation
Metal bindingi218 – 2181ManganeseUniRule annotation
Binding sitei218 – 2181SubstrateUniRule annotation
Metal bindingi237 – 2371Manganese; via tele nitrogenUniRule annotation
Binding sitei258 – 2581SubstrateUniRule annotation
Active sitei260 – 2601UniRule annotation
Metal bindingi275 – 2751ManganeseUniRule annotation
Binding sitei367 – 3671GTPUniRule annotation
Binding sitei398 – 3981GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2352-MONOMER.
BRENDAi4.1.1.32. 6163.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SSO2537
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103622Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273057.SSO2537.

Structurei

3D structure databases

ProteinModelPortaliQ97VS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni365 – 3673Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
InParanoidiQ97VS5.
KOiK01596.
OMAiWMRFGED.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97VS5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSSLDFLNN FIQRDAVKKL ESINNYPLIE FLNSVVKLCE PDSVYLITGS
60 70 80 90 100
DEEKEYIRKK ALESKEEIRL QTSGHTIHFD HPLDQARARE DTFILSDTKI
110 120 130 140 150
PYVNTKPRNE GLSEMLTLLK GSMRGREMYV GFYSLGPRNS PFQILAVQVT
160 170 180 190 200
DSPYVIHSEN ILYRIAFEDF SNNTKFLRFV HSKGEPDIKK RRIMIDLANN
210 220 230 240 250
TVYSVNTTYA GNSVGLKKLA LRLTIMKAVE EGWLSEHMAI VGFNGDKGIH
260 270 280 290 300
YFTASFPSGS GKTSTSMIGN LISDDLAFIR EFEGLPKAVN PEIGVFGIIQ
310 320 330 340 350
GINARDDPII WEVLHKPGEV IFSNVLMTED GDVYWEGSEL PKPERGYNHE
360 370 380 390 400
GRWDRESGKP ASHPNARFTV PLTSFSNLDK NWDNPNGVII DGIIFGVRDY
410 420 430 440 450
STLVPVVEAF SWSHGVITIG ASMESARTSA VIGKSDELEF NPMAILDFMP
460 470 480 490 500
ISLSRYLRNY LNFGKRLRKS PKIFGFNYFL KDDNKFLNSK EDKKVWVSWA
510 520 530 540 550
VKRVEETANA IYTPIGLIPF YEDLKALFKR VLGKEYGKEE YEKQFTIKLR
560 570 580 590 600
KYLEKTERII EIYLKFEDIP SEVINELKMQ KERIVDYINK YGDSVSPFRL

EKD
Length:603
Mass (Da):69,057
Last modified:September 26, 2001 - v1
Checksum:iDA4CE81C0BA84F13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42666.1.
PIRiC90426.
RefSeqiNP_343876.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42666; AAK42666; SSO2537.
GeneIDi1453993.
KEGGisso:SSO2537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK42666.1.
PIRiC90426.
RefSeqiNP_343876.1. NC_002754.1.

3D structure databases

ProteinModelPortaliQ97VS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2537.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42666; AAK42666; SSO2537.
GeneIDi1453993.
KEGGisso:SSO2537.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
InParanoidiQ97VS5.
KOiK01596.
OMAiWMRFGED.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciSSOL273057:GCH2-2352-MONOMER.
BRENDAi4.1.1.32. 6163.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiPCKG_SULSO
AccessioniPrimary (citable) accession number: Q97VS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.