Q97UB2 (ILVD_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	SSO3107

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103551
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q97UB2 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 059B2D16BAC62643	FASTA	558	59,422
	10 20 30 40 50 60 MPAKLNSPSR YHGIYNAPHR AFLRSVGLTD EEIGKPLVAI ATAWSEAGPC NFHTLALARV 70 80 90 100 110 120 AKEGTKEAGL SPLAFPTMVV NDNIGMGSEG MRYSLVSRDL IADMVEAQFN AHAFDGLVGI 130 140 150 160 170 180 GGCDKTTPGI LMAMARLNVP SIYIYGGSAE PGYFMGKRLT IEDVHEAIGA YLAKRITENE 190 200 210 220 230 240 LYEIEKRAHP TLGTCSGLFT ANTMGSMSEA LGMALPGSAS PTATSSRRVM YVKETGKALG 250 260 270 280 290 300 SLIENGIKSR EILTFEAFEN AITTLMAMGG STNAVLHLLA IAYEAGVKLT LDDFNRISKR 310 320 330 340 350 360 TPYIASMKPG GDYVMADLDE VGGVPVVLKK LLDAGLLHGD VLTVTGKTMK QNLEQYKYPN 370 380 390 400 410 420 VPHSHIVRDV KNPIKPRGGI VILKGSLAPE GAVIKVAATN VVKFEGKAKV YNSEDDAFKG 430 440 450 460 470 480 VQSGEVSEGE VVIIRYEGPK GAPGMPEMLR VTAAIMGAGL NNVALVTDGR FSGATRGPMV 490 500 510 520 530 540 GHVAPEAMVG GPIAIVEDGD TIVIDVESER LDLKLSEEEI KNRLKRWSPP SPRYKSGLLA 550 KYASLVSQAS MGAVTRPA

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK43209.1.
PIR	B99494.
RefSeq	NP_344419.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q97UB2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1453128.
GenomeReviews	Gene locus SSO3107 in contig AE006641_GR.
KEGG	sso:SSO3107.
NMPDR	fig|273057.1.peg.2842.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO3107-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_SULSO

Accession

Primary (citable) accession number: Q97UB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2001
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program