Reviewed, UniProtKB/Swiss-Prot Q97U94 (AGAL_SULSO)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase
      Short name=Alpha-Gal
    EC=3.2.1.22

Gene names

Name:	galS
Ordered Locus Names:	SSO3127

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the degradation of various alpha-linked galactosides such as raffinose, stachyose and melibiose.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Enzyme regulation	Completely inhibited by most divalent metal cations, however magnesium and molybdene have no effect.
Subunit structure	Trimer Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 36 family.
biophysicochemical properties	Kinetic parameters: KM=0.08 mM for para-nitrophenol-alpha-D-galactopyranoside KM=37.4 mM for para-nitrophenol-beta-L-arabinopyranoside pH dependence: Optimum pH is 5.0. Temperature dependence: Optimum temperature is 90 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Alpha-galactosidase		PRO_0000232659
Sites
	Active site	367	1	Probable
	Active site	425	1	Probable
Experimental info
	Mutagenesis	367	1	D → G: Hydrolase activity is below detection limit. Ref.2
	Mutagenesis	425	1	D → G: Slightly increase of hydrolasee activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q97U94-1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 4263C97145745121	FASTA	648	74,567
	10 20 30 40 50 60 MIWIEDENGN KYQCDSNGNC ENLAIVKVNT KEYNNDGKIY SIEGKSFVKL SKFPITLKLN 70 80 90 100 110 120 LKADQILSLT TQQIYSEVYG KAFTYYNQLA IDMEPVTEPP KELKYDIKSL DHEKYIRTYP 130 140 150 160 170 180 CWLYPVFNNI PDYTVFALVK SGNSYEAFFT LSNNYVTAYL FGDSVRLYTG FNTDEIKRSY 190 200 210 220 230 240 FLSIGTSDNP YKAIENAINI ASKETFTFKL RKEKGFPDKV MNGLGWCSWN AFLTKDLNEE 250 260 270 280 290 300 NLIKVVKGII ERGLRLNWVI IDDGWQDQNN DRAIRSLNPD NKKFPNGFKN TVRAIKSLGV 310 320 330 340 350 360 KYVGLWHAIN AHWGGMSQEL MKSLNVNGYF TNFLNSYVPS PNLEDAIGFY KAFDGNILRD 370 380 390 400 410 420 FDLVKVDNQW VIHAIYDSFP IGLASRNIQI ALQYSVGKDV INCMSMNPEN YCNYFYSNVM 430 440 450 460 470 480 RNSIDYVPFW KDGTKLHIMF NAYNSLLTSH IVYPDYDMFM SYDPYAKVHL VARVFSGGPI 490 500 510 520 530 540 YITDRHPERT NIELLRMAVL PNGEVIRVDE PALITEDLLF KDPLRERVLL KLKGKVKGYN 550 560 570 580 590 600 AIAFFNLNSG EVEEEYNNNE DYYYYKVFSG EFGVGKFNVK LRELDAEVVI TFPKSNRVIG 610 620 630 640 LREYILPPFT VIVSDNVVIP KADGTLLYVK DTELNEVKAR EGIPVTIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Identification of a novel alpha-galactosidase from the hyperthermophilic archaeon Sulfolobus solfataricus." Brouns S.J.J., Smits N., Wu H., Snijders A.P.L., Wright P.C., de Vos W.M., van der Oost J. J. Bacteriol. 188:2392-2399(2006) [PubMed: 16547025] [Abstract] Cited for: MUTAGENESIS OF ASP-367 AND ASP-425, CHARACTERIZATION. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006641 Genomic DNA. Translation: AAK43227.1. Different initiation.
PIR	D90496.
RefSeq	NP_344437.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Genome annotation databases
GeneID	1453146.
GenomeReviews	Gene locus SSO3127 in contig AE006641_GR.
KEGG	sso:SSO3127.
NMPDR	fig|273057.1.peg.2860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97U94.
OMA	Q97U94. RNSIDYV.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO3127-MON.
BRENDA	3.2.1.22. 2070.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR008811. Raffinose_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF05691. Raffinose_syn. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_SULSO

Accession

Primary (citable) accession number: Q97U94

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	May 5, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)