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Protein

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Gene

eda

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3-deoxygalactonate (KDGal).1 Publication

Catalytic activityi

2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.1 Publication
2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate.1 Publication

Pathwayi: 2-dehydro-3-deoxy-D-gluconate degradation

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase (kdgK)
  2. 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase (eda)
This subpathway is part of the pathway 2-dehydro-3-deoxy-D-gluconate degradation, which is itself part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei144 – 1441Proton shuttleBy similarity
Active sitei169 – 1691Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  • 2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity Source: UniProtKB
  • 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2983-MONOMER.
UniPathwayiUPA00856; UER00829.

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase (EC:4.1.2.551 Publication)
Gene namesi
Name:eda
Ordered Locus Names:SSO3197
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3083082-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolasePRO_0000422655Add
BLAST

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.By similarity

Protein-protein interaction databases

STRINGi273057.SSO3197.

Structurei

3D structure databases

ProteinModelPortaliQ97U28.
SMRiQ97U28. Positions 16-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 582Substrate bindingBy similarity
Regioni144 – 1463Substrate bindingBy similarity
Regioni169 – 1713Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. KDPG aldolase subfamily.Curated

Phylogenomic databases

eggNOGiarCOG04172. Archaea.
COG0329. LUCA.
HOGENOMiHOG000268172.
InParanoidiQ97U28.
KOiK11395.
OMAiPEIITPI.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97U28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRQGNLEEL WCLRMPEIIT PIITPFTKDN RIDKEKLKIH AENLIRKGID
60 70 80 90 100
KLFVNGTTGL GPSLSPEEKL ENLKAVYDVT NKIIFQVGGL NLDDAIRLAK
110 120 130 140 150
LSKDFDIVGI ASYAPYYYPR MSEKHLVKYF KTLCEVSPHP VYLYNYPTAT
160 170 180 190 200
GKDIDAKVAK EIGCFTGVKD TIENIIHTLD YKRLNPNMLV YSGSDMLIAT
210 220 230 240 250
VASTGLDGNV AAGSNYLPEV TVTIKKLAME RKIDEALKLQ FLHDEVIEAS
260 270 280 290 300
RIFGSLSSNY VLTKYFQGYD LGYPRPPIFP LDDEEERQLI KKVEGIRAKL

VELKILKE
Length:308
Mass (Da):34,795
Last modified:October 1, 2001 - v1
Checksum:i6A5DE1EFB91D3C80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK43294.1.
PIRiG90504.

Genome annotation databases

EnsemblBacteriaiAAK43294; AAK43294; SSO3197.
KEGGisso:SSO3197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK43294.1.
PIRiG90504.

3D structure databases

ProteinModelPortaliQ97U28.
SMRiQ97U28. Positions 16-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO3197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK43294; AAK43294; SSO3197.
KEGGisso:SSO3197.

Phylogenomic databases

eggNOGiarCOG04172. Archaea.
COG0329. LUCA.
HOGENOMiHOG000268172.
InParanoidiQ97U28.
KOiK11395.
OMAiPEIITPI.

Enzyme and pathway databases

UniPathwayiUPA00856; UER00829.
BioCyciSSOL273057:GCH2-2983-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation."
    Ahmed H., Ettema T.J., Tjaden B., Geerling A.C., van der Oost J., Siebers B.
    Biochem. J. 390:529-540(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiKDGA_SULSO
AccessioniPrimary (citable) accession number: Q97U28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: October 1, 2001
Last modified: May 11, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.