Reviewed, UniProtKB/Swiss-Prot Q97TZ8 (GUAAB_SULSO)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Synonyms:	guaA
Ordered Locus Names:	SSO3232

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_00345
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140251
Regions
	Domain	33 – 191	159	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97TZ8-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 02D30198868AC698	FASTA	367	41,383
	10 20 30 40 50 60 MFDPASFVEE IGPQLRQKLG NEKVLAAVSG GVDSTTAAVL AYNLLGDKVI PVLVDTGFLR 70 80 90 100 110 120 KNEAEKIKDY LSNILPNLII VDKKETFISE IEGIDDAEMK RKRFRELFYS TISSLMKKFN 130 140 150 160 170 180 ARYLMQGTIA ADWVETQGGI KTQHNVLVQI GIDTEKEWGF TLIEPLADLY KNEVRELARY 190 200 210 220 230 240 LKLPKEISER QPFPGPGLLV RAVGKLTREK LEVVREANDI VEKYLDPFNY SQYFAVSFES 250 260 270 280 290 300 YGNLVNLDGI DAFLYKARAT GVKGDVRAYG NIAKIECSNI NNVKNIIDTL VKYDITHVLC 310 320 330 340 350 360 TLDERNSGKY SVAIRAVTTE DFMTADYVRI PKEILSKISS EILQKIPNVK EVLYDVTSKP PATIEFE

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK43326.1.
PIR	G90508.
RefSeq	NP_344536.1.
3D structure databases
SMR	Q97TZ8. Positions 2-367.
ModBase	Search...
Genome annotation databases
GeneID	1453245.
GenomeReviews	Gene locus SSO3232 in contig AE006641_GR.
KEGG	sso:SSO3232.
NMPDR	fig|273057.1.peg.2959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	GFLRENE.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO2451-MONOMER.
BRENDA	6.3.5.2. 2070.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00958. GMP_synt_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_SULSO

Accession

Primary (citable) accession number: Q97TZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)