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Q97TX9 (CAS4_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
CRISPR-associated exonuclease Cas4

EC=3.1.-.-
Gene names
Name:cas4
Ordered Locus Names:SSO0001
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) By similarity. A 5' to 3' partially processive exonuclease. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA. Ref.2

Cofactor

Mg2+ or Mn2+ required for ssDNA activity. Ref.2

Binds 1 4Fe-4S cluster per subunit Probable. It may be important for protein stability. Ref.2

Subunit structure

Forms oligomers with a molecular weight of about 175 kDa. Ref.2

Sequence similarities

Belongs to the CRISPR-associated protein Cas4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202CRISPR-associated exonuclease Cas4
PRO_0000422224

Sites

Metal binding321Iron-sulfur (4Fe-4S) Probable
Metal binding1881Iron-sulfur (4Fe-4S) Probable
Metal binding1911Iron-sulfur (4Fe-4S) Probable
Metal binding1971Iron-sulfur (4Fe-4S) Probable

Experimental info

Mutagenesis991D → A: No nuclease activity. Ref.2

Secondary structure

................................ 202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q97TX9 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: C1E8868ACC093382

FASTA20223,239
        10         20         30         40         50         60 
MITEFLLKKK LEEHLSHVKE ENTIYVTDLV RCPRRVRYES EYKELAISQV YAPSAILGDI 

        70         80         90        100        110        120 
LHLGLESVLK GNFNAETEVE TLREINVGGK VYKIKGRADA IIRNDNGKSI VIEIKTSRSD 

       130        140        150        160        170        180 
KGLPLIHHKM QLQIYLWLFS AEKGILVYIT PDRIAEYEIN EPLDEATIVR LAEDTIMLQN 

       190        200 
SPRFNWECKY CIFSVICPAK LT 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006641 Genomic DNA. Translation: AAK43340.1.
PIRA90139.
RefSeqNP_341578.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4IC1X-ray2.35A/B/C/D/F/G/H/I/J/K1-202[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273057.SSO0001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK43340; AAK43340; SSO0001.
GeneID1455258.
KEGGsso:SSO0001.

Phylogenomic databases

eggNOGCOG1468.
HOGENOMHOG000223327.
KOK07464.
OMADWECRYC.
ProtClustDBCLSK625494.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-1-MONOMER.

Family and domain databases

InterProIPR013343. CRISPR-assoc_prot_Cas4.
[Graphical view]
TIGRFAMsTIGR00372. cas4. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAS4_SULSO
AccessionPrimary (citable) accession number: Q97TX9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references