Q97TX9 (CAS4_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CRISPR-associated exonuclease Cas4
EC=3.1.-.-

Gene names

Name:	cas4
Ordered Locus Names:	SSO0001

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) By similarity. A 5' to 3' partially processive exonuclease. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA. Ref.2
Cofactor	Mg2+ or Mn2+ required for ssDNA activity. Ref.2 Binds 1 4Fe-4S cluster per subunit Probable. It may be important for protein stability. Ref.2
Subunit structure	Forms oligomers with a molecular weight of about 175 kDa. Ref.2
Sequence similarities	Belongs to the CRISPR-associated protein Cas4 family.

Ontologies

Keywords
Biological process	Antiviral defense
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	CRISPR-associated exonuclease Cas4		PRO_0000422224
Sites
	Metal binding	32	1	Iron-sulfur (4Fe-4S) Probable
	Metal binding	188	1	Iron-sulfur (4Fe-4S) Probable
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Probable
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Probable
Experimental info
	Mutagenesis	99	1	D → A: No nuclease activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q97TX9 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: C1E8868ACC093382	FASTA	202	23,239
	10 20 30 40 50 60 MITEFLLKKK LEEHLSHVKE ENTIYVTDLV RCPRRVRYES EYKELAISQV YAPSAILGDI 70 80 90 100 110 120 LHLGLESVLK GNFNAETEVE TLREINVGGK VYKIKGRADA IIRNDNGKSI VIEIKTSRSD 130 140 150 160 170 180 KGLPLIHHKM QLQIYLWLFS AEKGILVYIT PDRIAEYEIN EPLDEATIVR LAEDTIMLQN 190 200 SPRFNWECKY CIFSVICPAK LT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with an iron-sulfur cluster." Zhang J., Kasciukovic T., White M.F. PLoS ONE 7:E47232-E47232(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN EXONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-99. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK43340.1.
PIR	A90139.
RefSeq	NP_341578.1. NC_002754.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	273057.SSO0001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455258.
KEGG	sso:SSO0001.
Phylogenomic databases
eggNOG	COG1468.
HOGENOM	HOG000223327.
KO	K07464.
OMA	DWECRYC.
ProtClustDB	CLSK625494.
Family and domain databases
InterPro	IPR013343. CRISPR-assoc_prot_Cas4. [Graphical view]
TIGRFAMs	TIGR00372. cas4. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAS4_SULSO

Accession

Primary (citable) accession number: Q97TX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2013
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program