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Q97TX9

- CAS4_SULSO

UniProt

Q97TX9 - CAS4_SULSO

Protein

CRISPR-associated exonuclease Cas4

Gene

cas4

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) By similarity. A 5' to 3' partially processive exonuclease. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA.By similarity1 Publication

    Cofactori

    Mg2+ or Mn2+ required for ssDNA activity.1 Publication
    Binds 1 4Fe-4S cluster per subunit Probable. It may be important for protein stability.1 PublicationCurated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi32 – 321Iron-sulfur (4Fe-4S)Curated
    Metal bindingi188 – 1881Iron-sulfur (4Fe-4S)Curated
    Metal bindingi191 – 1911Iron-sulfur (4Fe-4S)Curated
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)Curated

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. exonuclease activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-1-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CRISPR-associated exonuclease Cas4 (EC:3.1.-.-)
    Gene namesi
    Name:cas4
    Ordered Locus Names:SSO0001
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi99 – 991D → A: No nuclease activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202CRISPR-associated exonuclease Cas4PRO_0000422224Add
    BLAST

    Interactioni

    Subunit structurei

    Forms oligomers with a molecular weight of about 175 kDa.1 Publication

    Protein-protein interaction databases

    STRINGi273057.SSO0001.

    Structurei

    Secondary structure

    202
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 1616
    Beta strandi23 – 253
    Helixi26 – 294
    Helixi33 – 419
    Helixi43 – 464
    Helixi47 – 515
    Helixi53 – 7220
    Beta strandi75 – 8713
    Beta strandi90 – 10213
    Beta strandi110 – 1156
    Helixi126 – 13914
    Beta strandi142 – 1509
    Beta strandi153 – 1597
    Helixi165 – 17612
    Beta strandi180 – 1845
    Helixi188 – 1903
    Helixi194 – 1963

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IC1X-ray2.35A/B/C/D/F/G/H/I/J/K1-202[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1468.
    HOGENOMiHOG000223327.
    KOiK07464.
    OMAiWECRYCP.

    Family and domain databases

    InterProiIPR013343. CRISPR-assoc_prot_Cas4.
    [Graphical view]
    TIGRFAMsiTIGR00372. cas4. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q97TX9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITEFLLKKK LEEHLSHVKE ENTIYVTDLV RCPRRVRYES EYKELAISQV    50
    YAPSAILGDI LHLGLESVLK GNFNAETEVE TLREINVGGK VYKIKGRADA 100
    IIRNDNGKSI VIEIKTSRSD KGLPLIHHKM QLQIYLWLFS AEKGILVYIT 150
    PDRIAEYEIN EPLDEATIVR LAEDTIMLQN SPRFNWECKY CIFSVICPAK 200
    LT 202
    Length:202
    Mass (Da):23,239
    Last modified:October 1, 2001 - v1
    Checksum:iC1E8868ACC093382
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK43340.1.
    PIRiA90139.
    RefSeqiNP_341578.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK43340; AAK43340; SSO0001.
    GeneIDi1455258.
    KEGGisso:SSO0001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK43340.1 .
    PIRi A90139.
    RefSeqi NP_341578.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IC1 X-ray 2.35 A/B/C/D/F/G/H/I/J/K 1-202 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273057.SSO0001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK43340 ; AAK43340 ; SSO0001 .
    GeneIDi 1455258.
    KEGGi sso:SSO0001.

    Phylogenomic databases

    eggNOGi COG1468.
    HOGENOMi HOG000223327.
    KOi K07464.
    OMAi WECRYCP.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-1-MONOMER.

    Family and domain databases

    InterProi IPR013343. CRISPR-assoc_prot_Cas4.
    [Graphical view ]
    TIGRFAMsi TIGR00372. cas4. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with an iron-sulfur cluster."
      Zhang J., Kasciukovic T., White M.F.
      PLoS ONE 7:E47232-E47232(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN EXONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-99.
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

    Entry informationi

    Entry nameiCAS4_SULSO
    AccessioniPrimary (citable) accession number: Q97TX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3