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Q97TX9

- CAS4_SULSO

UniProt

Q97TX9 - CAS4_SULSO

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Protein
CRISPR-associated exonuclease Cas4
Gene
cas4, SSO0001
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) By similarity. A 5' to 3' partially processive exonuclease. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA.1 Publication

Cofactori

Mg2+ or Mn2+ required for ssDNA activity.1 Publication
Binds 1 4Fe-4S cluster per subunit Inferred. It may be important for protein stability.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi32 – 321Iron-sulfur (4Fe-4S) Inferred
Metal bindingi188 – 1881Iron-sulfur (4Fe-4S) Inferred
Metal bindingi191 – 1911Iron-sulfur (4Fe-4S) Inferred
Metal bindingi197 – 1971Iron-sulfur (4Fe-4S) Inferred

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. exonuclease activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-1-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated exonuclease Cas4 (EC:3.1.-.-)
Gene namesi
Name:cas4
Ordered Locus Names:SSO0001
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 991D → A: No nuclease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202CRISPR-associated exonuclease Cas4
PRO_0000422224Add
BLAST

Interactioni

Subunit structurei

Forms oligomers with a molecular weight of about 175 kDa.1 Publication

Protein-protein interaction databases

STRINGi273057.SSO0001.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1616
Beta strandi23 – 253
Helixi26 – 294
Helixi33 – 419
Helixi43 – 464
Helixi47 – 515
Helixi53 – 7220
Beta strandi75 – 8713
Beta strandi90 – 10213
Beta strandi110 – 1156
Helixi126 – 13914
Beta strandi142 – 1509
Beta strandi153 – 1597
Helixi165 – 17612
Beta strandi180 – 1845
Helixi188 – 1903
Helixi194 – 1963

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IC1X-ray2.35A/B/C/D/F/G/H/I/J/K1-202[»]

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1468.
HOGENOMiHOG000223327.
KOiK07464.
OMAiWECRYCP.

Family and domain databases

InterProiIPR013343. CRISPR-assoc_prot_Cas4.
[Graphical view]
TIGRFAMsiTIGR00372. cas4. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97TX9-1 [UniParc]FASTAAdd to Basket

« Hide

MITEFLLKKK LEEHLSHVKE ENTIYVTDLV RCPRRVRYES EYKELAISQV    50
YAPSAILGDI LHLGLESVLK GNFNAETEVE TLREINVGGK VYKIKGRADA 100
IIRNDNGKSI VIEIKTSRSD KGLPLIHHKM QLQIYLWLFS AEKGILVYIT 150
PDRIAEYEIN EPLDEATIVR LAEDTIMLQN SPRFNWECKY CIFSVICPAK 200
LT 202
Length:202
Mass (Da):23,239
Last modified:October 1, 2001 - v1
Checksum:iC1E8868ACC093382
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK43340.1.
PIRiA90139.
RefSeqiNP_341578.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK43340; AAK43340; SSO0001.
GeneIDi1455258.
KEGGisso:SSO0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK43340.1 .
PIRi A90139.
RefSeqi NP_341578.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IC1 X-ray 2.35 A/B/C/D/F/G/H/I/J/K 1-202 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273057.SSO0001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK43340 ; AAK43340 ; SSO0001 .
GeneIDi 1455258.
KEGGi sso:SSO0001.

Phylogenomic databases

eggNOGi COG1468.
HOGENOMi HOG000223327.
KOi K07464.
OMAi WECRYCP.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-1-MONOMER.

Family and domain databases

InterProi IPR013343. CRISPR-assoc_prot_Cas4.
[Graphical view ]
TIGRFAMsi TIGR00372. cas4. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with an iron-sulfur cluster."
    Zhang J., Kasciukovic T., White M.F.
    PLoS ONE 7:E47232-E47232(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN EXONUCLEASE, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-99.
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiCAS4_SULSO
AccessioniPrimary (citable) accession number: Q97TX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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