Q97TN4 (ALF1_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	CA_P0064

Encoded on

Plasmid pSOL1

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP-Rule MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP-Rule MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Fructose-bisphosphate aldolase class 1 HAMAP-Rule MF_00729		PRO_0000216899
Sites
	Active site	176	1	Proton acceptor By similarity
	Active site	213	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97TN4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 75C866B87CB11551	FASTA	295	33,505
	10 20 30 40 50 60 MNETQMNRIS KDKGFIAALD QSGGSTPKAL LQYGIKENSY SNDEEMFNLV HEMRKRIIKS 70 80 90 100 110 120 TAFNSKYILG AILFENTMYR TIDHKYTADY LWNEKNIVPF LKIDKGLSEL ENGVQLMKPI 130 140 150 160 170 180 TNLDELLKAA IEKNIFGTKM RSFIKEANSK GIKMVVDQQF ELADKIANQG LVPIIEPEVD 190 200 210 220 230 240 IKSTDKEKSE ELLKLEISKH LSDLDKETKV MLKLSIPTKD NFYSDLMKDP HVVRIVALSG 250 260 270 280 290 GYSQSEANER LSRNNGLIAS FSRALSENLS IDQTDEEFNE TLSNSIKEIY EASIT

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001438 Genomic DNA. Translation: AAK76810.1.
RefSeq	NP_149228.1. NC_001988.2.
3D structure databases
ProteinModelPortal	Q97TN4.
SMR	Q97TN4. Positions 1-295.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_P0064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK76810; AAK76810; CA_P0064.
GeneID	1116069.
KEGG	cac:CA_P0064.
PATRIC	32034012. VBICloAce74127_0064.
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000073502.
KO	K01623.
OMA	GTKMRSV.
ProtClustDB	PRK05377.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-3909-MONOMER.
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00729. FBP_aldolase_1.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. IPR023014. FBP_aldolase_I_bac. [Graphical view]
Pfam	PF00274. Glycolytic. 2 hits. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_CLOAB

Accession

Primary (citable) accession number: Q97TN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program