Reviewed, UniProtKB/Swiss-Prot Q97TN4 (ALF1_CLOAB)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase class 1
    EC=4.1.2.13
Alternative name(s):
    Fructose-biphosphate aldolase class I
      Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	CA_P0064

Encoded on

Plasmid pSOL1

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Fructose-bisphosphate aldolase class 1 HAMAP MF_00729		PRO_0000216899
Sites
	Active site	176	1	Proton acceptor By similarity
	Active site	213	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97TN4-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 75C866B87CB11551	FASTA	295	33,505
	10 20 30 40 50 60 MNETQMNRIS KDKGFIAALD QSGGSTPKAL LQYGIKENSY SNDEEMFNLV HEMRKRIIKS 70 80 90 100 110 120 TAFNSKYILG AILFENTMYR TIDHKYTADY LWNEKNIVPF LKIDKGLSEL ENGVQLMKPI 130 140 150 160 170 180 TNLDELLKAA IEKNIFGTKM RSFIKEANSK GIKMVVDQQF ELADKIANQG LVPIIEPEVD 190 200 210 220 230 240 IKSTDKEKSE ELLKLEISKH LSDLDKETKV MLKLSIPTKD NFYSDLMKDP HVVRIVALSG 250 260 270 280 290 GYSQSEANER LSRNNGLIAS FSRALSENLS IDQTDEEFNE TLSNSIKEIY EASIT

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001438 Genomic DNA. Translation: AAK76810.1.
RefSeq	NP_149228.1.
3D structure databases
SMR	Q97TN4. Positions 1-295.
ModBase	Search...
Genome annotation databases
GeneID	1116069.
GenomeReviews	Gene locus CA_P0064 in contig AE001438_GR.
KEGG	cac:CA_P0064.
NMPDR	fig|272562.1.peg.63.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG307137.
OMA	GTKMRSV.
Enzyme and pathway databases
BioCyc	CACE272562:CAP0064-MONOMER.
BRENDA	4.1.2.13. 2866.
Family and domain databases
HAMAP	MF_00729. FBP_aldolase_1. [Tree]
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 2 hits. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_CLOAB

Accession

Primary (citable) accession number: Q97TN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	October 1, 2001
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)