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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.By similarity

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATP; via amide nitrogen and carbonyl oxygen1
Active sitei103NucleophileBy similarity1
Binding sitei127ATP; via amide nitrogen1
Active sitei200Cysteine persulfide intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19ATP8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmA (EC:2.8.1.13)
Gene namesi
Name:mnmA
Synonyms:trmU
Ordered Locus Names:SP_0118
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001216851 – 373tRNA-specific 2-thiouridylase MnmAAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi103 ↔ 200AlternateBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei128Interaction with tRNABy similarity1
Sitei344Interaction with tRNABy similarity1

Protein-protein interaction databases

STRINGi170187.SpneT_02001784.

Structurei

Secondary structure

1373
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi7 – 12Combined sources6
Helixi17 – 28Combined sources12
Beta strandi32 – 39Combined sources8
Helixi51 – 66Combined sources16
Beta strandi70 – 74Combined sources5
Helixi76 – 82Combined sources7
Helixi84 – 92Combined sources9
Helixi99 – 106Combined sources8
Turni107 – 110Combined sources4
Helixi111 – 117Combined sources7
Turni118 – 120Combined sources3
Beta strandi122 – 125Combined sources4
Beta strandi129 – 134Combined sources6
Beta strandi136 – 138Combined sources3
Beta strandi140 – 144Combined sources5
Turni148 – 150Combined sources3
Helixi153 – 156Combined sources4
Helixi161 – 164Combined sources4
Turni170 – 173Combined sources4
Helixi176 – 185Combined sources10
Turni189 – 192Combined sources4
Turni200 – 204Combined sources5
Helixi207 – 212Combined sources6
Beta strandi220 – 224Combined sources5
Beta strandi229 – 234Combined sources6
Helixi236 – 238Combined sources3
Turni247 – 250Combined sources4
Beta strandi260 – 266Combined sources7
Helixi267 – 269Combined sources3
Beta strandi271 – 277Combined sources7
Helixi281 – 283Combined sources3
Beta strandi284 – 297Combined sources14
Beta strandi301 – 312Combined sources12
Beta strandi318 – 324Combined sources7
Beta strandi329 – 338Combined sources10
Beta strandi344 – 350Combined sources7
Beta strandi353 – 369Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HMAX-ray2.41A1-373[»]
ProteinModelPortaliQ97T38.
SMRiQ97T38.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97T38.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Interaction with target base in tRNABy similarity3
Regioni150 – 152Interaction with tRNABy similarity3
Regioni244 – 253Interaction with tRNABy similarity10
Regioni312 – 313Interaction with tRNABy similarity2

Sequence similaritiesi

Belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218046.
KOiK00566.
OMAiLHKINFA.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97T38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNSKTRVV VGMSGGVDSS VTALLLKEQG YDVIGIFMKN WDDTDENGVC
60 70 80 90 100
TATEDYKDVV AVADQIGIPY YSVNFEKEYW DRVFEYFLAE YRAGRTPNPD
110 120 130 140 150
VMCNKEIKFK AFLDYAITLG ADYVATGHYA RVARDEDGTV HMLRGVDNGK
160 170 180 190 200
DQTYFLSQLS QEQLQKTMFP LGHLEKPEVR RLAEEAGLST AKKKDSTGIC
210 220 230 240 250
FIGEKNFKNF LSNYLPAQPG RMMTVDGRDM GEHAGLMYYT IGQRGGLGIG
260 270 280 290 300
GQHGGDNAPW FVVGKDLSKN ILYVGQGFYH DSLMSTSLEA SQVHFTREMP
310 320 330 340 350
EEFTLECTAK FRYRQPDSKV TVHVKGEKTE VIFAEPQRAI TPGQAVVFYD
360 370
GEECLGGGLI DNAYRDGQVC QYI
Length:373
Mass (Da):41,682
Last modified:October 1, 2001 - v1
Checksum:i8C1E1A81603D426F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74304.1.
PIRiG95013.
RefSeqiWP_001282969.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK74304; AAK74304; SP_0118.
KEGGispn:SP_0118.
PATRICi19704563. VBIStrPne105772_0126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74304.1.
PIRiG95013.
RefSeqiWP_001282969.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HMAX-ray2.41A1-373[»]
ProteinModelPortaliQ97T38.
SMRiQ97T38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02001784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74304; AAK74304; SP_0118.
KEGGispn:SP_0118.
PATRICi19704563. VBIStrPne105772_0126.

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218046.
KOiK00566.
OMAiLHKINFA.

Miscellaneous databases

EvolutionaryTraceiQ97T38.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMA_STRPN
AccessioniPrimary (citable) accession number: Q97T38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.