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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.By similarity

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATP; via amide nitrogen and carbonyl oxygen
Active sitei103 – 1031NucleophileBy similarity
Binding sitei127 – 1271ATP; via amide nitrogen
Active sitei200 – 2001Cysteine persulfide intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-124-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmA (EC:2.8.1.13)
Gene namesi
Name:mnmA
Synonyms:trmU
Ordered Locus Names:SP_0118
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373tRNA-specific 2-thiouridylase MnmAPRO_0000121685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi103 ↔ 200AlternateBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei128 – 1281Interaction with tRNABy similarity
Sitei344 – 3441Interaction with tRNABy similarity

Protein-protein interaction databases

STRINGi170187.SpneT_02001784.

Structurei

Secondary structure

1
373
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi7 – 126Combined sources
Helixi17 – 2812Combined sources
Beta strandi32 – 398Combined sources
Helixi51 – 6616Combined sources
Beta strandi70 – 745Combined sources
Helixi76 – 827Combined sources
Helixi84 – 929Combined sources
Helixi99 – 1068Combined sources
Turni107 – 1104Combined sources
Helixi111 – 1177Combined sources
Turni118 – 1203Combined sources
Beta strandi122 – 1254Combined sources
Beta strandi129 – 1346Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi140 – 1445Combined sources
Turni148 – 1503Combined sources
Helixi153 – 1564Combined sources
Helixi161 – 1644Combined sources
Turni170 – 1734Combined sources
Helixi176 – 18510Combined sources
Turni189 – 1924Combined sources
Turni200 – 2045Combined sources
Helixi207 – 2126Combined sources
Beta strandi220 – 2245Combined sources
Beta strandi229 – 2346Combined sources
Helixi236 – 2383Combined sources
Turni247 – 2504Combined sources
Beta strandi260 – 2667Combined sources
Helixi267 – 2693Combined sources
Beta strandi271 – 2777Combined sources
Helixi281 – 2833Combined sources
Beta strandi284 – 29714Combined sources
Beta strandi301 – 31212Combined sources
Beta strandi318 – 3247Combined sources
Beta strandi329 – 33810Combined sources
Beta strandi344 – 3507Combined sources
Beta strandi353 – 36917Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HMAX-ray2.41A1-373[»]
ProteinModelPortaliQ97T38.
SMRiQ97T38. Positions 2-373.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97T38.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1003Interaction with target base in tRNABy similarity
Regioni150 – 1523Interaction with tRNABy similarity
Regioni244 – 25310Interaction with tRNABy similarity
Regioni312 – 3132Interaction with tRNABy similarity

Sequence similaritiesi

Belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218046.
KOiK00566.
OMAiLHKINFA.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97T38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNSKTRVV VGMSGGVDSS VTALLLKEQG YDVIGIFMKN WDDTDENGVC
60 70 80 90 100
TATEDYKDVV AVADQIGIPY YSVNFEKEYW DRVFEYFLAE YRAGRTPNPD
110 120 130 140 150
VMCNKEIKFK AFLDYAITLG ADYVATGHYA RVARDEDGTV HMLRGVDNGK
160 170 180 190 200
DQTYFLSQLS QEQLQKTMFP LGHLEKPEVR RLAEEAGLST AKKKDSTGIC
210 220 230 240 250
FIGEKNFKNF LSNYLPAQPG RMMTVDGRDM GEHAGLMYYT IGQRGGLGIG
260 270 280 290 300
GQHGGDNAPW FVVGKDLSKN ILYVGQGFYH DSLMSTSLEA SQVHFTREMP
310 320 330 340 350
EEFTLECTAK FRYRQPDSKV TVHVKGEKTE VIFAEPQRAI TPGQAVVFYD
360 370
GEECLGGGLI DNAYRDGQVC QYI
Length:373
Mass (Da):41,682
Last modified:October 1, 2001 - v1
Checksum:i8C1E1A81603D426F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74304.1.
PIRiG95013.
RefSeqiWP_001282969.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK74304; AAK74304; SP_0118.
KEGGispn:SP_0118.
PATRICi19704563. VBIStrPne105772_0126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74304.1.
PIRiG95013.
RefSeqiWP_001282969.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HMAX-ray2.41A1-373[»]
ProteinModelPortaliQ97T38.
SMRiQ97T38. Positions 2-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02001784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74304; AAK74304; SP_0118.
KEGGispn:SP_0118.
PATRICi19704563. VBIStrPne105772_0126.

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218046.
KOiK00566.
OMAiLHKINFA.

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-124-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ97T38.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMA_STRPN
AccessioniPrimary (citable) accession number: Q97T38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.