Putative alkaline amylopullulanase - Streptococcus pneumoniae TIGR4

Preferred order of sections

Names and origin

Protein names

Submitted name:
Putative alkaline amylopullulanase EMBL AAK74446.1

Gene names

Ordered Locus Names:

SP_0268

Organism

Streptococcus pneumoniae TIGR4 [Complete proteome] EMBL AAK74446.1

Taxonomic identifier

170187 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1280 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location

Secreted › cell wall; Peptidoglycan-anchor By similarity SAAS SAAS019931.

Ontologies

Keywords
Cellular component	Secreted SAAS SAAS019931
Ligand	Calcium PDB 2YA2 PDB 2YA0 Metal-binding PDB 2J44 PDB 2YA2 PDB 2YA0 Zinc PDB 2J44
Technical term	3D-structure PDB 2J44 PDB 2YA2 PDB 2YA0 PDB 2YA1 Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

156 – 158

3

Glucose binding PDB 2J44 PDB 2YA1

Region

263 – 265

3

Glucose binding PDB 2J44 PDB 2YA1

Region

839 – 842

4

Glucose binding PDB 2YA1

Region

989 – 991

3

Glucose binding PDB 2YA2 PDB 2YA1

Sites

Metal binding

281

1

Zinc PDB 2J44

Metal binding

310

1

Zinc PDB 2J44

Metal binding

661

1

Calcium 1; via carbonyl oxygen PDB 2YA0

Metal binding

663

1

Calcium 1; via carbonyl oxygen PDB 2YA0

Metal binding

828

1

Calcium 2; via carbonyl oxygen PDB 2YA2

Metal binding

831

1

Calcium 2; via carbonyl oxygen PDB 2YA2

Metal binding

832

1

Calcium 2 PDB 2YA2

Metal binding

882

1

Calcium 2 PDB 2YA2

Metal binding

886

1

Calcium 2 PDB 2YA2

Metal binding

992

1

Calcium 1 PDB 2YA0

Binding site

168

1

Glucose PDB 2J44 PDB 2YA1

Binding site

214

1

Glucose PDB 2J44 PDB 2YA1

Binding site

276

1

Glucose PDB 2J44 PDB 2YA1

Binding site

318

1

Glucose PDB 2J44 PDB 2YA1

Binding site

323

1

Glucose PDB 2J44 PDB 2YA1

Binding site

668

1

Glucose PDB 2YA2

Binding site

743

1

Glucose; via amide nitrogen PDB 2YA1

Binding site

809

1

Glucose PDB 2YA1

Binding site

896

1

Glucose PDB 2YA1

Binding site

969

1

Glucose PDB 2YA1

Sequences

Sequence

Length

Mass (Da)

Tools

Q97SQ7 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: A209B802A8E0B328
Show »

FASTA

1,280

142,619

        10         20         30         40         50         60 
MRKTPSHTEK KMVYSIRSLK NGTGSVLIGA SLVLLAMATP TISSDESTPT TNEPNNRNTT 

        70         80         90        100        110        120 
TLAQPLTDTA AGSGKNESDI SSPGNANASL EKTEEKPAAS PADPAPQTGQ DRSSEPTTST 

       130        140        150        160        170        180 
SPVTTETKAE EPIEDNYFRI HVKKLPEENK DAQGLWTWDD VEKPSENWPN GALSFKDAKK 

       190        200        210        220        230        240 
DDYGYYLDVK LKGEQAKKIS FLINNTAGKN LTGDKSVEKL VPKMNEAWLD QDYKVFSYEP 

       250        260        270        280        290        300 
QPAGTVRVNY YRTDGNYDKK SLWYWGDVKN PSSAQWPDGT DFTATGKYGR YIDIPLNEAA 

       310        320        330        340        350        360 
REFGFLLLDE SKQGDDVKIR KENYKFTDLK NHSQIFLKDD DESIYTNPYY VHDIRMTGAQ 

       370        380        390        400        410        420 
HVGTSSIESS FSTLVGAKKE DILKHSNITN HLGNKVTITD VAIDEAGKKV TYSGDFSDTK 

       430        440        450        460        470        480 
HPYTVSYNSD QFTTKTSWRL KDETYSYDGK LGADLKEEGK QVDLTLWSPS ADKVSVVVYD 

       490        500        510        520        530        540 
KNDPDKVVGT VALEKGERGT WKQTLDSTNK LGITDFTGYY YQYQIERQGK TVLALDPYAK 

       550        560        570        580        590        600 
SLAAWNSDDS KIDDAHKVAK AAFVDPAKLG PQDLTYGKIH NFKTREDAVI YEAHVRDFTS 

       610        620        630        640        650        660 
DPAIAKDLTK PFGTFEAFIE KLDYLKDLGV THIQLLPVLS YYFVNELKNH ERLSDYASSN 

       670        680        690        700        710        720 
SNYNWGYDPQ NYFSLTGMYS SDPKNPEKRI AEFKNLINEI HKRGMGAILD VVYNHTAKVD 

       730        740        750        760        770        780 
LFEDLEPNYY HFMDADGTPR TSFGGGRLGT THHMTKRLLI DSIKYLVDTY KVDGFRFDMM 

       790        800        810        820        830        840 
GDHDAASIEE AYKAARALNP NLIMLGEGWR TYAGDENMPT KAADQDWMKH TDTVAVFSDD 

       850        860        870        880        890        900 
IRNNLKSGYP NEGQPAFITG GKRDVNTIFK NLIAQPTNFE ADSPGDVIQY IAAHDNLTLF 

       910        920        930        940        950        960 
DIIAQSIKKD PSKAENYAEI HRRLRLGNLM VLTAQGTPFI HSGQEYGRTK QFRDPAYKTP 

       970        980        990       1000       1010       1020 
VAEDKVPNKS HLLRDKDGNP FDYPYFIHDS YDSSDAVNKF DWTKATDGKA YPENVKSRDY 

      1030       1040       1050       1060       1070       1080 
MKGLIALRQS TDAFRLKSLQ DIKDRVHLIT VPGQNGVEKE DVVIGYQITA PNGDIYAVFV 

      1090       1100       1110       1120       1130       1140 
NADEKAREFN LGTAFAHLRN AEVLADENQA GPVGIANPKG LEWTEKGLKL NALTATVLRV 

      1150       1160       1170       1180       1190       1200 
SQNGTSHEST AEEKPDSTPS KPEHQNEASH PAHQDPAPEA RPDSTKPDAK VADAENKPSQ 

      1210       1220       1230       1240       1250       1260 
ATADSQAEQP AQEAQASSVK EAVRNESVEN SSKENIPATP DKQAELPNTG IKNENKLLFA 

      1270       1280 
GISLLALLGL GFLLKNKKEN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S., Heidelberg J., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.
[2]	"Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors." van Bueren A.L., Higgins M., Wang D., Burke R.D., Boraston A.B. Nat. Struct. Mol. Biol. 14:76-84(2007) [PubMed: 17187076] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 135-353 IN COMPLEX WITH GLUCOSE AND ZINC.
[3]	"The Conformation and Function of a Multi-Modular Glycogen-Degrading Pneumococcal Virulence Factor." Lammerts Van Bueren A., Ficko-Blean E., Pluvinage B., Hehemann J.H., Higgins M.A., Deng L., Ogunniyi A.D., Stroeher U.H., Warry N.E., Burke R.D., Czjzek M., Paton J.C., Vocadlo D.J., Boraston A.B. Submitted (FEB-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 437-1150 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE005672 Genomic DNA. Translation: AAK74446.1.

PIR

E95031.

RefSeq

NP_344806.1. NC_003028.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J44	X-ray	2.10	A	135-353	[»]
2YA0	X-ray	1.85	A	437-1150	[»]
2YA1	X-ray	2.25	A	135-1143	[»]
2YA2	X-ray	2.37	A	436-1143	[»]

ProteinModelPortal

Q97SQ7.

SMR

Q97SQ7. Positions 135-353.

ModBase

Search...

Protein family/group databases

CAZy

CBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTRT00000025252; EBSTRP00000024344; EBSTRG00000025252.

GeneID

930075.

GenomeReviews

Gene locus SP_0268 in contig AE005672_GR.

KEGG

spn:SP_0268.

PATRIC

19704869. VBIStrPne105772_0280.

TIGR

SP_0268.

Phylogenomic databases

GeneTree

EBGT00050000026660.

HOGENOM

HBG575037.

OMA

KSGFPNE.

ProtClustDB

CLSK803694.

Enzyme and pathway databases

BioCyc

SPNE170187-1:SP_0268-MONOMER.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR005877. Gpos_YSIRK.
IPR019948. Gram-positive_anchor.
IPR013783. Ig-like_fold.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR005323. PUD.
IPR011838. Pullulan_Gpos.
IPR001899. Surface_protein_Gram_pos_cocci.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

KO

K01176.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 2 hits.
PF02922. CBM_48. 1 hit.
PF00746. Gram_pos_anchor. 1 hit.
PF03714. PUD. 2 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

TIGRFAMs

TIGR01167. LPXTG_anchor. 1 hit.
TIGR02102. Pullulan_Gpos. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.

PROSITE

PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q97SQ7_STRPN

Accession

Primary (citable) accession number: Q97SQ7

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2001
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)