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Protein

Antitoxin PezA

Gene

pezA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Upon expression in E.coli neutralizes the toxic effect of cognate toxin PezT. Represses transcription of its own operon, PezT acts as a corepressor, considerably increasing repression.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi17 – 36H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin PezA
Gene namesi
Name:pezA
Ordered Locus Names:SP_1050
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004109661 – 158Antitoxin PezAAdd BLAST158

Expressioni

Inductioni

Conflicting data is available; found to be a member of the pezAT operon (upon ectopic expression in E.coli); in S.pneumoniae strain 0100993 is found in a monocistronic operon.

Interactioni

Subunit structurei

Probably a homodimer, forms a PezA2PezT2 heterotetramer. The heterotetramer is much more stable than either of the proteins alone, and a specific mechanism may be necessary to liberate the toxin.2 Publications

Protein-protein interaction databases

DIPiDIP-58970N.
IntActiQ97QZ2. 1 interactor.
STRINGi170187.SpneT_02000929.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi69 – 98Combined sources30
Turni99 – 101Combined sources3
Helixi110 – 122Combined sources13
Turni123 – 125Combined sources3
Helixi126 – 128Combined sources3
Helixi132 – 154Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20A/C/E/G1-158[»]
ProteinModelPortaliQ97QZ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97QZ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 60HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1396. LUCA.
HOGENOMiHOG000267565.
KOiK18830.
OMAiKEFAMIG.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
1.10.8.130. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR015090. Epsilon_antitox.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97QZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGKNIKSLR KTHDLTQLEF ARIVGISRNS LSRYENGTSS VSTELIDIIC
60 70 80 90 100
QKFNVSYVDI VGEDKMLNPV EDYELTLKIE IVKERGANLL SRLYRYQDSQ
110 120 130 140 150
GISIDDESNP WILMSDDLSD LIHTNIYLVE TFDEIERYSG YLDGIERMLE

ISEKRMVA
Length:158
Mass (Da):18,248
Last modified:October 1, 2001 - v1
Checksum:i0ABB5257446881C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75164.1.
PIRiC95121.
RefSeqiWP_000579608.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75164; AAK75164; SP_1050.
KEGGispn:SP_1050.
PATRICi19706507. VBIStrPne105772_1098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75164.1.
PIRiC95121.
RefSeqiWP_000579608.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20A/C/E/G1-158[»]
ProteinModelPortaliQ97QZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58970N.
IntActiQ97QZ2. 1 interactor.
STRINGi170187.SpneT_02000929.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75164; AAK75164; SP_1050.
KEGGispn:SP_1050.
PATRICi19706507. VBIStrPne105772_1098.

Phylogenomic databases

eggNOGiCOG1396. LUCA.
HOGENOMiHOG000267565.
KOiK18830.
OMAiKEFAMIG.

Miscellaneous databases

EvolutionaryTraceiQ97QZ2.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
1.10.8.130. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR015090. Epsilon_antitox.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEZA_STRPN
AccessioniPrimary (citable) accession number: Q97QZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: October 1, 2001
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.