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Q97QZ1 (PEZT_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toxin PezT
Alternative name(s):
UDP-N-acetylglucosamine kinase
Short name=UNAG kinase
EC=2.7.1.176
Zeta toxin
Gene names
Name:pezT
Ordered Locus Names:SP_1051
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Upon expression in E.coli results in decreased cell growth and viability, followed 3 hours later by growth restoration; the toxic effect and phosphorylation of UNAG are neutralized by coexpression with cognate antitoxin PezA. A mutant lacking the last 11 residues is stably maintained in E.coli, unlike the wild-type which undergoes spontaneous mutation. Expression of the deletion mutant in rapidly growing liquid cultures leads to cell bulging, permeabilization and massive lysis by 1 hour. Cells that survive are not able to undergo cytokinesis. Expression in slowly growing cells leads to bulging but not lysis. Ref.4 Ref.5

Acts as a corepressor of its own operon with PezA; it is not clear if it binds DNA alone. Ref.4 Ref.5

Catalytic activity

ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

Subunit structure

Forms a PezA2PezT2 heterotetramer. The heterotetramer is much more stable than either of the proteins alone, and a specific mechanism may be necessary to liberate the toxin. Ref.3 Ref.5

Induction

Conflicting data is available; found to be a member of the pezAT operon (upon ectopic expression in E.coli); in S.pneumoniae strain 0100993 is found in an operon with the 2 following genes (SP_1052 and SP_1053).

Disruption phenotype

Strains with a pezT/SP_1052/SP_1053 disruption have partially attenuated virulence, they take longer to develop a terminal infection in mice, although they grow normally in liquid culture. A double SP_1052/SP_1053 disruption grows almost as well as wild-type, showing the effect is mostly due to disruption of pezT. Ref.2

Sequence similarities

Belongs to the zeta toxin family.

Ontologies

Keywords
   Biological processVirulence
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Repressor
Toxin
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 253253Toxin PezT
PRO_0000410967

Regions

Nucleotide binding39 – 468ATP Potential

Sites

Active site661Proton acceptor Probable

Experimental info

Mutagenesis451K → A: Abolishes lethality. Ref.5
Mutagenesis661D → T: Abolishes lethality. Ref.5
Mutagenesis1171T → V: Abolishes lethality. Ref.5
Mutagenesis1201T → V: Very slight reduction in toxic effect. Ref.5
Mutagenesis1571R → A: Abolishes lethality. Ref.5
Mutagenesis1701R → A: Abolishes lethality. Ref.5
Mutagenesis243 – 25311Missing: Retains toxicity while being stably maintained in E.coli. Ref.4

Secondary structure

...................................... 253
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q97QZ1 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 0F7CD8D0B26EE89E

FASTA25329,129
        10         20         30         40         50         60 
MEIQDYTDSE FKHALARNLR SLTRGKKSSK QPIAILLGGQ SGAGKTTIHR IKQKEFQGNI 

        70         80         90        100        110        120 
VIIDGDSFRS QHPHYLELQQ EYGKDSVEYT KDFAGKMVES LVTKLSSLRY NLLIEGTLRT 

       130        140        150        160        170        180 
VDVPKKTAQL LKNKGYEVQL ALIATKPELS YLSTLIRYEE LYIINPNQAR ATPKEHHDFI 

       190        200        210        220        230        240 
VNHLVDNTRK LEELAIFERI QIYQRDRSCV YDSKENTTSA ADVLQELLFG EWSQVEKEML 

       250 
QVGEKRLNEL LEK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.
[2]"A locus contained within a variable region of pneumococcal pathogenicity island 1 contributes to virulence in mice."
Brown J.S., Gilliland S.M., Spratt B.G., Holden D.W.
Infect. Immun. 72:1587-1593(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, OPERON STRUCTURE.
Strain: 0100993 / NCIMB 40794 / Serotype 3.
[3]"Assembly dynamics and stability of the pneumococcal epsilon zeta antitoxin toxin (PezAT) system from Streptococcus pneumoniae."
Mutschler H., Reinstein J., Meinhart A.
J. Biol. Chem. 285:21797-21806(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, COMPLEX STABILITY.
Strain: ATCC BAA-334 / TIGR4.
[4]"A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis."
Mutschler H., Gebhardt M., Shoeman R.L., Meinhart A.
PLoS Biol. 9:E1001033-E1001033(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPRESSION IN E.COLI, FUNCTION AS A UNAG KINASE, MUTAGENESIS OF 243-GLY--LYS-253.
Strain: ATCC BAA-334 / TIGR4.
[5]"Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae."
Khoo S.K., Loll B., Chan W.T., Shoeman R.L., Ngoo L., Yeo C.C., Meinhart A.
J. Biol. Chem. 282:19606-19618(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS), EXPRESSION IN E.COLI, FUNCTION AS A TOXIN, FUNCTION AS A TRANSCRIPTIONAL COREPRESSOR, SUBUNIT, OPERON STRUCTURE, MUTAGENESIS OF LYS-45; ASP-66; THR-117; THR-120; ARG-157 AND ARG-170.
Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005672 Genomic DNA. Translation: AAK75165.1.
PIRD95121.
RefSeqNP_345525.1. NC_003028.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20B/D/F/H1-253[»]
ProteinModelPortalQ97QZ1.
SMRQ97QZ1. Positions 1-251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-58971N.
STRING170187.SP_1051.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75165; AAK75165; SP_1051.
GeneID931565.
KEGGspn:SP_1051.
PATRIC19706509. VBIStrPne105772_1099.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000267564.
KOK16214.
OMAMLMATLM.
OrthoDBEOG6FBWVB.
ProtClustDBCLSK803780.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1056-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ97QZ1.

Entry information

Entry namePEZT_STRPN
AccessionPrimary (citable) accession number: Q97QZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references