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Q97QZ1

- PEZT_STRPN

UniProt

Q97QZ1 - PEZT_STRPN

Protein

Toxin PezT

Gene

pezT

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Upon expression in E.coli results in decreased cell growth and viability, followed 3 hours later by growth restoration; the toxic effect and phosphorylation of UNAG are neutralized by coexpression with cognate antitoxin PezA. A mutant lacking the last 11 residues is stably maintained in E.coli, unlike the wild-type which undergoes spontaneous mutation. Expression of the deletion mutant in rapidly growing liquid cultures leads to cell bulging, permeabilization and massive lysis by 1 hour. Cells that survive are not able to undergo cytokinesis. Expression in slowly growing cells leads to bulging but not lysis.
    Acts as a corepressor of its own operon with PezA; it is not clear if it binds DNA alone.

    Catalytic activityi

    ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Proton acceptorCurated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 468ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Repressor, Toxin, Transferase

    Keywords - Biological processi

    Virulence

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-1056-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toxin PezT
    Alternative name(s):
    UDP-N-acetylglucosamine kinase (EC:2.7.1.176)
    Short name:
    UNAG kinase
    Zeta toxin
    Gene namesi
    Name:pezT
    Ordered Locus Names:SP_1051
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Strains with a pezT/SP_1052/SP_1053 disruption have partially attenuated virulence, they take longer to develop a terminal infection in mice, although they grow normally in liquid culture. A double SP_1052/SP_1053 disruption grows almost as well as wild-type, showing the effect is mostly due to disruption of pezT.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi45 – 451K → A: Abolishes lethality. 2 Publications
    Mutagenesisi66 – 661D → T: Abolishes lethality. 2 Publications
    Mutagenesisi117 – 1171T → V: Abolishes lethality. 2 Publications
    Mutagenesisi120 – 1201T → V: Very slight reduction in toxic effect. 2 Publications
    Mutagenesisi157 – 1571R → A: Abolishes lethality. 2 Publications
    Mutagenesisi170 – 1701R → A: Abolishes lethality. 2 Publications
    Mutagenesisi243 – 25311Missing: Retains toxicity while being stably maintained in E.coli. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 253253Toxin PezTPRO_0000410967Add
    BLAST

    Expressioni

    Inductioni

    Conflicting data is available; found to be a member of the pezAT operon (upon ectopic expression in E.coli); in S.pneumoniae strain 0100993 is found in an operon with the 2 following genes (SP_1052 and SP_1053).

    Interactioni

    Subunit structurei

    Forms a PezA2PezT2 heterotetramer. The heterotetramer is much more stable than either of the proteins alone, and a specific mechanism may be necessary to liberate the toxin.2 Publications

    Protein-protein interaction databases

    DIPiDIP-58971N.
    STRINGi170187.SP_1051.

    Structurei

    Secondary structure

    1
    253
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2316
    Beta strandi33 – 397
    Helixi41 – 433
    Helixi46 – 5510
    Turni56 – 583
    Beta strandi61 – 633
    Helixi65 – 695
    Helixi75 – 795
    Helixi87 – 10721
    Beta strandi112 – 1154
    Helixi122 – 13312
    Beta strandi137 – 1437
    Helixi147 – 16014
    Turni161 – 1633
    Helixi181 – 19313
    Beta strandi198 – 2036
    Beta strandi209 – 2124
    Turni213 – 2153
    Helixi220 – 22910
    Helixi234 – 25017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P5TX-ray3.20B/D/F/H1-253[»]
    ProteinModelPortaliQ97QZ1.
    SMRiQ97QZ1. Positions 1-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ97QZ1.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the zeta toxin family.Curated

    Phylogenomic databases

    HOGENOMiHOG000267564.
    KOiK16214.
    OMAiMLMATLM.
    OrthoDBiEOG6FBWVB.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR010488. Zeta_toxin_domain.
    [Graphical view]
    PfamiPF06414. Zeta_toxin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q97QZ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIQDYTDSE FKHALARNLR SLTRGKKSSK QPIAILLGGQ SGAGKTTIHR    50
    IKQKEFQGNI VIIDGDSFRS QHPHYLELQQ EYGKDSVEYT KDFAGKMVES 100
    LVTKLSSLRY NLLIEGTLRT VDVPKKTAQL LKNKGYEVQL ALIATKPELS 150
    YLSTLIRYEE LYIINPNQAR ATPKEHHDFI VNHLVDNTRK LEELAIFERI 200
    QIYQRDRSCV YDSKENTTSA ADVLQELLFG EWSQVEKEML QVGEKRLNEL 250
    LEK 253
    Length:253
    Mass (Da):29,129
    Last modified:October 1, 2001 - v1
    Checksum:i0F7CD8D0B26EE89E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK75165.1.
    PIRiD95121.
    RefSeqiNP_345525.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK75165; AAK75165; SP_1051.
    GeneIDi931565.
    KEGGispn:SP_1051.
    PATRICi19706509. VBIStrPne105772_1099.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK75165.1 .
    PIRi D95121.
    RefSeqi NP_345525.1. NC_003028.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P5T X-ray 3.20 B/D/F/H 1-253 [» ]
    ProteinModelPortali Q97QZ1.
    SMRi Q97QZ1. Positions 1-251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-58971N.
    STRINGi 170187.SP_1051.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK75165 ; AAK75165 ; SP_1051 .
    GeneIDi 931565.
    KEGGi spn:SP_1051.
    PATRICi 19706509. VBIStrPne105772_1099.

    Phylogenomic databases

    HOGENOMi HOG000267564.
    KOi K16214.
    OMAi MLMATLM.
    OrthoDBi EOG6FBWVB.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-1056-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q97QZ1.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR010488. Zeta_toxin_domain.
    [Graphical view ]
    Pfami PF06414. Zeta_toxin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.
    2. "A locus contained within a variable region of pneumococcal pathogenicity island 1 contributes to virulence in mice."
      Brown J.S., Gilliland S.M., Spratt B.G., Holden D.W.
      Infect. Immun. 72:1587-1593(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, OPERON STRUCTURE.
      Strain: 0100993 / NCIMB 40794 / Serotype 3.
    3. "Assembly dynamics and stability of the pneumococcal epsilon zeta antitoxin toxin (PezAT) system from Streptococcus pneumoniae."
      Mutschler H., Reinstein J., Meinhart A.
      J. Biol. Chem. 285:21797-21806(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, COMPLEX STABILITY.
      Strain: ATCC BAA-334 / TIGR4.
    4. "A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis."
      Mutschler H., Gebhardt M., Shoeman R.L., Meinhart A.
      PLoS Biol. 9:E1001033-E1001033(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: EXPRESSION IN E.COLI, FUNCTION AS A UNAG KINASE, MUTAGENESIS OF 243-GLY--LYS-253.
      Strain: ATCC BAA-334 / TIGR4.
    5. "Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae."
      Khoo S.K., Loll B., Chan W.T., Shoeman R.L., Ngoo L., Yeo C.C., Meinhart A.
      J. Biol. Chem. 282:19606-19618(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS), EXPRESSION IN E.COLI, FUNCTION AS A TOXIN, FUNCTION AS A TRANSCRIPTIONAL COREPRESSOR, SUBUNIT, OPERON STRUCTURE, MUTAGENESIS OF LYS-45; ASP-66; THR-117; THR-120; ARG-157 AND ARG-170.
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiPEZT_STRPN
    AccessioniPrimary (citable) accession number: Q97QZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3