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Protein

Toxin PezT

Gene

pezT

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Upon expression in E.coli results in decreased cell growth and viability, followed 3 hours later by growth restoration; the toxic effect and phosphorylation of UNAG are neutralized by coexpression with cognate antitoxin PezA. A mutant lacking the last 11 residues is stably maintained in E.coli, unlike the wild-type which undergoes spontaneous mutation. Expression of the deletion mutant in rapidly growing liquid cultures leads to cell bulging, permeabilization and massive lysis by 1 hour. Cells that survive are not able to undergo cytokinesis. Expression in slowly growing cells leads to bulging but not lysis.
Acts as a corepressor of its own operon with PezA; it is not clear if it binds DNA alone.

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Proton acceptorCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Toxin, Transferase

Keywords - Biological processi

Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.176. 1960.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin PezT
Alternative name(s):
UDP-N-acetylglucosamine kinase (EC:2.7.1.176)
Short name:
UNAG kinase
Zeta toxin
Gene namesi
Name:pezT
Ordered Locus Names:SP_1051
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Strains with a pezT/SP_1052/SP_1053 disruption have partially attenuated virulence, they take longer to develop a terminal infection in mice, although they grow normally in liquid culture. A double SP_1052/SP_1053 disruption grows almost as well as wild-type, showing the effect is mostly due to disruption of pezT.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45K → A: Abolishes lethality. 1 Publication1
Mutagenesisi66D → T: Abolishes lethality. 1 Publication1
Mutagenesisi117T → V: Abolishes lethality. 1 Publication1
Mutagenesisi120T → V: Very slight reduction in toxic effect. 1 Publication1
Mutagenesisi157R → A: Abolishes lethality. 1 Publication1
Mutagenesisi170R → A: Abolishes lethality. 1 Publication1
Mutagenesisi243 – 253Missing : Retains toxicity while being stably maintained in E.coli. 1 PublicationAdd BLAST11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004109671 – 253Toxin PezTAdd BLAST253

Expressioni

Inductioni

Conflicting data is available; found to be a member of the pezAT operon (upon ectopic expression in E.coli); in S.pneumoniae strain 0100993 is found in an operon with the 2 following genes (SP_1052 and SP_1053).

Interactioni

Subunit structurei

Forms a PezA2PezT2 heterotetramer. The heterotetramer is much more stable than either of the proteins alone, and a specific mechanism may be necessary to liberate the toxin.2 Publications

Protein-protein interaction databases

DIPiDIP-58971N.
STRINGi170187.SpneT_02000930.

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 23Combined sources16
Beta strandi33 – 39Combined sources7
Helixi41 – 43Combined sources3
Helixi46 – 55Combined sources10
Turni56 – 58Combined sources3
Beta strandi61 – 63Combined sources3
Helixi65 – 69Combined sources5
Helixi75 – 79Combined sources5
Helixi87 – 107Combined sources21
Beta strandi112 – 115Combined sources4
Helixi122 – 133Combined sources12
Beta strandi137 – 143Combined sources7
Helixi147 – 160Combined sources14
Turni161 – 163Combined sources3
Helixi181 – 193Combined sources13
Beta strandi198 – 203Combined sources6
Beta strandi209 – 212Combined sources4
Turni213 – 215Combined sources3
Helixi220 – 229Combined sources10
Helixi234 – 250Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20B/D/F/H1-253[»]
ProteinModelPortaliQ97QZ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ97QZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the zeta toxin family.Curated

Phylogenomic databases

eggNOGiENOG4108WZA. Bacteria.
ENOG4111J1Q. LUCA.
HOGENOMiHOG000267564.
KOiK16214.
OMAiAGKMVEQ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97QZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIQDYTDSE FKHALARNLR SLTRGKKSSK QPIAILLGGQ SGAGKTTIHR
60 70 80 90 100
IKQKEFQGNI VIIDGDSFRS QHPHYLELQQ EYGKDSVEYT KDFAGKMVES
110 120 130 140 150
LVTKLSSLRY NLLIEGTLRT VDVPKKTAQL LKNKGYEVQL ALIATKPELS
160 170 180 190 200
YLSTLIRYEE LYIINPNQAR ATPKEHHDFI VNHLVDNTRK LEELAIFERI
210 220 230 240 250
QIYQRDRSCV YDSKENTTSA ADVLQELLFG EWSQVEKEML QVGEKRLNEL

LEK
Length:253
Mass (Da):29,129
Last modified:October 1, 2001 - v1
Checksum:i0F7CD8D0B26EE89E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75165.1.
PIRiD95121.
RefSeqiWP_000405360.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75165; AAK75165; SP_1051.
KEGGispn:SP_1051.
PATRICi19706509. VBIStrPne105772_1099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75165.1.
PIRiD95121.
RefSeqiWP_000405360.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20B/D/F/H1-253[»]
ProteinModelPortaliQ97QZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58971N.
STRINGi170187.SpneT_02000930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75165; AAK75165; SP_1051.
KEGGispn:SP_1051.
PATRICi19706509. VBIStrPne105772_1099.

Phylogenomic databases

eggNOGiENOG4108WZA. Bacteria.
ENOG4111J1Q. LUCA.
HOGENOMiHOG000267564.
KOiK16214.
OMAiAGKMVEQ.

Enzyme and pathway databases

BRENDAi2.7.1.176. 1960.

Miscellaneous databases

EvolutionaryTraceiQ97QZ1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPEZT_STRPN
AccessioniPrimary (citable) accession number: Q97QZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.