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Q97QZ1

- PEZT_STRPN

UniProt

Q97QZ1 - PEZT_STRPN

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Protein

Toxin PezT

Gene
pezT, SP_1051
Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Upon expression in E.coli results in decreased cell growth and viability, followed 3 hours later by growth restoration; the toxic effect and phosphorylation of UNAG are neutralized by coexpression with cognate antitoxin PezA. A mutant lacking the last 11 residues is stably maintained in E.coli, unlike the wild-type which undergoes spontaneous mutation. Expression of the deletion mutant in rapidly growing liquid cultures leads to cell bulging, permeabilization and massive lysis by 1 hour. Cells that survive are not able to undergo cytokinesis. Expression in slowly growing cells leads to bulging but not lysis.2 Publications
Acts as a corepressor of its own operon with PezA; it is not clear if it binds DNA alone.2 Publications

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptor Inferred

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Toxin, Transferase

Keywords - Biological processi

Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1056-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin PezT
Alternative name(s):
UDP-N-acetylglucosamine kinase (EC:2.7.1.176)
Short name:
UNAG kinase
Zeta toxin
Gene namesi
Name:pezT
Ordered Locus Names:SP_1051
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

Pathology & Biotechi

Disruption phenotypei

Strains with a pezT/SP_1052/SP_1053 disruption have partially attenuated virulence, they take longer to develop a terminal infection in mice, although they grow normally in liquid culture. A double SP_1052/SP_1053 disruption grows almost as well as wild-type, showing the effect is mostly due to disruption of pezT.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 451K → A: Abolishes lethality. 1 Publication
Mutagenesisi66 – 661D → T: Abolishes lethality. 1 Publication
Mutagenesisi117 – 1171T → V: Abolishes lethality. 1 Publication
Mutagenesisi120 – 1201T → V: Very slight reduction in toxic effect. 1 Publication
Mutagenesisi157 – 1571R → A: Abolishes lethality. 1 Publication
Mutagenesisi170 – 1701R → A: Abolishes lethality. 1 Publication
Mutagenesisi243 – 25311Missing: Retains toxicity while being stably maintained in E.coli. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Toxin PezTPRO_0000410967Add
BLAST

Expressioni

Inductioni

Conflicting data is available; found to be a member of the pezAT operon (upon ectopic expression in E.coli); in S.pneumoniae strain 0100993 is found in an operon with the 2 following genes (SP_1052 and SP_1053).

Interactioni

Subunit structurei

Forms a PezA2PezT2 heterotetramer. The heterotetramer is much more stable than either of the proteins alone, and a specific mechanism may be necessary to liberate the toxin.2 Publications

Protein-protein interaction databases

DIPiDIP-58971N.
STRINGi170187.SP_1051.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2316
Beta strandi33 – 397
Helixi41 – 433
Helixi46 – 5510
Turni56 – 583
Beta strandi61 – 633
Helixi65 – 695
Helixi75 – 795
Helixi87 – 10721
Beta strandi112 – 1154
Helixi122 – 13312
Beta strandi137 – 1437
Helixi147 – 16014
Turni161 – 1633
Helixi181 – 19313
Beta strandi198 – 2036
Beta strandi209 – 2124
Turni213 – 2153
Helixi220 – 22910
Helixi234 – 25017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P5TX-ray3.20B/D/F/H1-253[»]
ProteinModelPortaliQ97QZ1.
SMRiQ97QZ1. Positions 1-251.

Miscellaneous databases

EvolutionaryTraceiQ97QZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the zeta toxin family.

Phylogenomic databases

HOGENOMiHOG000267564.
KOiK16214.
OMAiMLMATLM.
OrthoDBiEOG6FBWVB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view]
PfamiPF06414. Zeta_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97QZ1-1 [UniParc]FASTAAdd to Basket

« Hide

MEIQDYTDSE FKHALARNLR SLTRGKKSSK QPIAILLGGQ SGAGKTTIHR    50
IKQKEFQGNI VIIDGDSFRS QHPHYLELQQ EYGKDSVEYT KDFAGKMVES 100
LVTKLSSLRY NLLIEGTLRT VDVPKKTAQL LKNKGYEVQL ALIATKPELS 150
YLSTLIRYEE LYIINPNQAR ATPKEHHDFI VNHLVDNTRK LEELAIFERI 200
QIYQRDRSCV YDSKENTTSA ADVLQELLFG EWSQVEKEML QVGEKRLNEL 250
LEK 253
Length:253
Mass (Da):29,129
Last modified:October 1, 2001 - v1
Checksum:i0F7CD8D0B26EE89E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005672 Genomic DNA. Translation: AAK75165.1.
PIRiD95121.
RefSeqiNP_345525.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK75165; AAK75165; SP_1051.
GeneIDi931565.
KEGGispn:SP_1051.
PATRICi19706509. VBIStrPne105772_1099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005672 Genomic DNA. Translation: AAK75165.1 .
PIRi D95121.
RefSeqi NP_345525.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2P5T X-ray 3.20 B/D/F/H 1-253 [» ]
ProteinModelPortali Q97QZ1.
SMRi Q97QZ1. Positions 1-251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-58971N.
STRINGi 170187.SP_1051.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK75165 ; AAK75165 ; SP_1051 .
GeneIDi 931565.
KEGGi spn:SP_1051.
PATRICi 19706509. VBIStrPne105772_1099.

Phylogenomic databases

HOGENOMi HOG000267564.
KOi K16214.
OMAi MLMATLM.
OrthoDBi EOG6FBWVB.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-1056-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q97QZ1.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR010488. Zeta_toxin_domain.
[Graphical view ]
Pfami PF06414. Zeta_toxin. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.
  2. "A locus contained within a variable region of pneumococcal pathogenicity island 1 contributes to virulence in mice."
    Brown J.S., Gilliland S.M., Spratt B.G., Holden D.W.
    Infect. Immun. 72:1587-1593(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, OPERON STRUCTURE.
    Strain: 0100993 / NCIMB 40794 / Serotype 3.
  3. "Assembly dynamics and stability of the pneumococcal epsilon zeta antitoxin toxin (PezAT) system from Streptococcus pneumoniae."
    Mutschler H., Reinstein J., Meinhart A.
    J. Biol. Chem. 285:21797-21806(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, COMPLEX STABILITY.
    Strain: ATCC BAA-334 / TIGR4.
  4. "A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis."
    Mutschler H., Gebhardt M., Shoeman R.L., Meinhart A.
    PLoS Biol. 9:E1001033-E1001033(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION IN E.COLI, FUNCTION AS A UNAG KINASE, MUTAGENESIS OF 243-GLY--LYS-253.
    Strain: ATCC BAA-334 / TIGR4.
  5. "Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae."
    Khoo S.K., Loll B., Chan W.T., Shoeman R.L., Ngoo L., Yeo C.C., Meinhart A.
    J. Biol. Chem. 282:19606-19618(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS), EXPRESSION IN E.COLI, FUNCTION AS A TOXIN, FUNCTION AS A TRANSCRIPTIONAL COREPRESSOR, SUBUNIT, OPERON STRUCTURE, MUTAGENESIS OF LYS-45; ASP-66; THR-117; THR-120; ARG-157 AND ARG-170.
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiPEZT_STRPN
AccessioniPrimary (citable) accession number: Q97QZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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