Reviewed, UniProtKB/Swiss-Prot Q97NQ4 (MURA1_STRPN)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Synonyms:	murA
Ordered Locus Names:	SP_1966

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000178930
Sites
	Active site	120	1	Proton donor By similarity
	Binding site	120	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97NQ4-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: F0E341DAB4B89C2E	FASTA	427	45,868
	10 20 30 40 50 60 MDKIVVQGGD NRLVGSVTIE GAKNAVLPLL AATILASEGK TVLQNVPILS DVFIMNQVVG 70 80 90 100 110 120 GLNAKVDFDE EAHLVKVDAT GDITEEAPYK YVSKMRASIV VLGPILARVG HAKVSMPGGC 130 140 150 160 170 180 TIGSRPIDLH LKGLEAMGVK ISQTAGYIEA KAERLHGAHI YMDFPSVGAT QNLMMAATLA 190 200 210 220 230 240 DGVTVIENAA REPEIVDLAI LLNEMGAKVK GAGTETITIT GVEKLHGTTH NVVQDRIEAG 250 260 270 280 290 300 TFMVAAAMTG GDVLIRDAVW EHNRPLIAKL LEMGVEVIEE DEGIRVRSQL ENLKAVHVKT 310 320 330 340 350 360 LPHPGFPTDM QAQFTALMTV AKGESTMVET VFENRFQHLE EMRRMGLHSE IIRDTARIVG 370 380 390 400 410 420 GQPLQGAEVL STDLRASAAL ILTGLVAQGE TVVGKLVHLD RGYYGFHEKL AQLGAKIQRI EASDEDE

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK76033.1.
PIR	H95229.
RefSeq	NP_346393.1.
3D structure databases
SMR	Q97NQ4. Positions 1-422.
ModBase	Search...
Genome annotation databases
GeneID	931882.
GenomeReviews	Gene locus SP_1966 in contig AE005672_GR.
KEGG	spn:SP_1966.
TIGR	SP_1966.
Phylogenomic databases
HOGENOM	HBG482701.
OMA	VSRVYHL.
Enzyme and pathway databases
BRENDA	2.5.1.7. 600.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_STRPN

Accession

Primary (citable) accession number: Q97NQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)