Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-Ala-D/L-Glu epimerase

Gene

CA_C0192

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp.1 Publication

Catalytic activityi

L-alanyl-D-glutamate = L-alanyl-L-glutamate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: peptidoglycan degradation

This protein is involved in the pathway peptidoglycan degradation, which is part of Cell wall degradation.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan degradation and in Cell wall degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei160 – 1601SubstrateBy similarity
Active sitei162 – 1621Proton acceptor; specific for (R)-substrate epimerizationBy similarity
Metal bindingi190 – 1901MagnesiumBy similarity
Metal bindingi218 – 2181MagnesiumBy similarity
Metal bindingi243 – 2431MagnesiumBy similarity
Active sitei267 – 2671Proton acceptor; specific for (S)-substrate epimerizationBy similarity
Binding sitei295 – 2951Substrate; via carbonyl oxygenBy similarity
Binding sitei320 – 3201SubstrateBy similarity
Binding sitei322 – 3221SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-214-MONOMER.
UniPathwayiUPA00549.

Names & Taxonomyi

Protein namesi
Recommended name:
L-Ala-D/L-Glu epimerase (EC:5.1.1.201 Publication)
Short name:
AE epimerase
Short name:
AEE
Gene namesi
Ordered Locus Names:CA_C0192
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358L-Ala-D/L-Glu epimerasePRO_0000388971Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_C0192.

Structurei

3D structure databases

ProteinModelPortaliQ97MK4.
SMRiQ97MK4. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000185903.
KOiK19802.
OMAiCMVGSMI.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97MK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIKDIVIGH LSVPLKKPFK TAVRSVNSVN DVVVKIITDT GNVGFGSAAS
60 70 80 90 100
TGLVTGDITE SIEGAINNYI KRSIVGMDIE DFEAILIKLD NCIVGNTSAK
110 120 130 140 150
AAVDIALYDL YGQRYGAPLY KLLGGFRNKL ETDITISVNS PEEMSRDSVD
160 170 180 190 200
AVKLGYKTLK IKVGKNPKLD IKRMREIRKA IGYEVNLRID ANQGWQPKEA
210 220 230 240 250
IRALNEIENE GLKIELVEQP VKAWNLEGLK MVTDNVNIPV MADESVFSPK
260 270 280 290 300
DAARVMEMRA CDLINIKLMK TGGIHNALKI CALAEVYGME CMLGCMLEGK
310 320 330 340 350
VSVTAAVHLA AAKRIITKID LDGPVLCSRD DVVGGAMYDN SNIVLVDEPG

LGIEGINN
Length:358
Mass (Da):38,905
Last modified:October 1, 2001 - v1
Checksum:i6BE3980A1836DD23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK78174.1.
PIRiC96923.
RefSeqiNP_346834.1. NC_003030.1.
WP_010963516.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78174; AAK78174; CA_C0192.
GeneIDi1116375.
KEGGicac:CA_C0192.
PATRICi32034681. VBICloAce74127_0378.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK78174.1.
PIRiC96923.
RefSeqiNP_346834.1. NC_003030.1.
WP_010963516.1. NC_003030.1.

3D structure databases

ProteinModelPortaliQ97MK4.
SMRiQ97MK4. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C0192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK78174; AAK78174; CA_C0192.
GeneIDi1116375.
KEGGicac:CA_C0192.
PATRICi32034681. VBICloAce74127_0378.

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000185903.
KOiK19802.
OMAiCMVGSMI.

Enzyme and pathway databases

UniPathwayiUPA00549.
BioCyciCACE272562:GJIH-214-MONOMER.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAEEP_CLOAB
AccessioniPrimary (citable) accession number: Q97MK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.