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Q97MI6

- BIOB_CLOAB

UniProt

Q97MI6 - BIOB_CLOAB

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Protein
Biotin synthase
Gene
bioB, CA_C0210
Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi112 – 1121Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi147 – 1471Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi207 – 2071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi277 – 2771Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCACE272562:GJIH-232-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CA_C0210
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Biotin synthaseUniRule annotation
PRO_0000381308Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C0210.

Structurei

3D structure databases

ProteinModelPortaliQ97MI6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97MI6-1 [UniParc]FASTAAdd to Basket

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MKNQWNNILS IGEKVLNGEK ITADEALTLS KSNGSDIFLL CSFANKLREK    50
FNGNHVDLCS VINAKSGNCS EDCAFCAQSA HHKANVSCYP LLNEDKILEM 100
AKQREAYGAR HCDIATSGLG YTGDEKDFQT ILKAFKKMKE NTNLKLCACL 150
GTLTEKAMNS LAAVGVERYN HNLETAKSFY KNIVSTHGYD ERIKTINYAK 200
NAKMEVCSGM IVGLGETMEQ RIEHALLLRD LNVDAVPVNI LNPVKGTKLE 250
NAKPLSPMEI IKTFAIIRFI LPDKIIRYAG GREKNLRSLQ PLGFLSGLNG 300
MLIGNYLTTN GQSVNDDFNM LKDLELEY 328
Length:328
Mass (Da):36,428
Last modified:October 1, 2001 - v1
Checksum:i0C51144AB6D72C42
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78192.1.
PIRiE96925.
RefSeqiNP_346852.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78192; AAK78192; CA_C0210.
GeneIDi1116393.
KEGGicac:CA_C0210.
PATRICi32034715. VBICloAce74127_0395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK78192.1 .
PIRi E96925.
RefSeqi NP_346852.1. NC_003030.1.

3D structure databases

ProteinModelPortali Q97MI6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272562.CA_C0210.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK78192 ; AAK78192 ; CA_C0210 .
GeneIDi 1116393.
KEGGi cac:CA_C0210.
PATRICi 32034715. VBICloAce74127_0395.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CACE272562:GJIH-232-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiBIOB_CLOAB
AccessioniPrimary (citable) accession number: Q97MI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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