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Protein

L-lactate dehydrogenase 1

Gene

ldh1

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldh2), L-lactate dehydrogenase 1 (ldh1)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89SubstrateBy similarity1
Binding sitei121NAD or substrateBy similarity1
Binding sitei152SubstrateBy similarity1
Active sitei176Proton acceptorBy similarity1
Binding sitei231SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 41NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1 (EC:1.1.1.27)
Short name:
L-LDH 1
Gene namesi
Name:ldh1
Synonyms:ldh
Ordered Locus Names:CA_C0267
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683331 – 313L-lactate dehydrogenase 1Add BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272562.CA_C0267.

Structurei

3D structure databases

ProteinModelPortaliQ97MD1.
SMRiQ97MD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97MD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNTKISVI GAGFVGSSTV FALMNGGLAS EIVIVDVNKD KAEGEAMDLS
60 70 80 90 100
HGAAFVKPVV VKSGDYKDTE GSDIVIITAG AAQKPGETRL ELINKNYNIF
110 120 130 140 150
KSIVPEVVKY NPNAILLVVS NPVDILTYIT YKLSGFPKSR VIGSGTVLDT
160 170 180 190 200
SRFRYMLSEH FEIDVRNIHT YIMGEHGDSE IATWSLTNIA GMDVNEYCEA
210 220 230 240 250
SCKKCDGSLK YKIYDDVKNA AYHVIEKKGA TYYAVALAVK RIVEAILRDE
260 270 280 290 300
NSILTVSSLL EGQYGIKDVY MGVPSIVGIN GVKDIIEVPL NDEEKNNLTD
310
SAKTLKESLD SIF
Length:313
Mass (Da):34,181
Last modified:October 1, 2001 - v1
Checksum:iC98FD402E62F45D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK78248.1.
PIRiE96932.
RefSeqiNP_346908.1. NC_003030.1.
WP_010963590.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78248; AAK78248; CA_C0267.
GeneIDi1116450.
KEGGicac:CA_C0267.
PATRICifig|272562.8.peg.453.

Similar proteinsi

Entry informationi

Entry nameiLDH1_CLOAB
AccessioniPrimary (citable) accession number: Q97MD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2001
Last modified: October 25, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families