Q97LR2 (ALR1_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase 1
EC=5.1.1.1

Gene names

Name:	alr1
Ordered Locus Names:	CA_C0492

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Alanine racemase 1 HAMAP MF_01201		PRO_0000114509
Sites
	Active site	38	1	Proton acceptor; specific for D-alanine By similarity
	Active site	267	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	38	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97LR2 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 3EBADBE597A4D41B	FASTA	386	43,736
	10 20 30 40 50 60 MFRHIRPVWA EIDLDNIAYN MQQIRRCSKS DEIIGVVKAD AYGHGAVDVA PVLLENGANR 70 80 90 100 110 120 LAVAVISEAV ELRKSGIQCP IMILGYTPLS LVDSIIKYSI EQTVFSYDYA EKLSEAARQK 130 140 150 160 170 180 NITLRIHIAL DTGMGRIGFL PTEESVWEVY KISKLSNIII EGIFSHFSTS DETNKEYTYA 190 200 210 220 230 240 QLKKFEWFYN ELRKKNIKIN IRHIGNSAAI MELPETHFEA TRPGIILYGY YPSNEVDKNK 250 260 270 280 290 300 LNLKPIMTLK TNVVHIKKMM PGEYVSYGRK FKCERESIIA TLPVGYADGY TRMLSGKAKV 310 320 330 340 350 360 IINGNYAPVI GRICMDQCMI DITDLPSVQV GDEVVIMGES DDKKFTADDM AEIIGTINYE 370 380 VICMISKRVP RVYIKNGEVV KIRNYV

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK78472.1.
PIR	E96960.
RefSeq	NP_347132.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97LR2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1116675.
GenomeReviews	Gene locus CA_C0492 in contig AE001437_GR.
KEGG	cac:CA_C0492.
NMPDR	fig|272562.1.peg.661.
PATRIC	32035339. VBICloAce74127_0691.
Phylogenomic databases
HOGENOM	HBG712172.
OMA	NWQIKAL.
PhylomeDB	Q97LR2.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	CACE272562:CAC0492-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_CLOAB

Accession

Primary (citable) accession number: Q97LR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program