Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138L-tryptophanUniRule annotation1
Binding sitei189ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 15ATPUniRule annotation3
Nucleotide bindingi21 – 22ATPUniRule annotation2
Nucleotide bindingi150 – 152ATPUniRule annotation3
Nucleotide bindingi198 – 202ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:CA_C0626
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366211 – 335Tryptophan--tRNA ligaseAdd BLAST335

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C0626

Structurei

3D structure databases

ProteinModelPortaliQ97LD6
SMRiQ97LD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 22"HIGH" regionUniRule annotation9
Motifi198 – 202"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q97LD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGENKKVIFS GIQPSGELTI GNYFGAIKNW VKLQDEYDCY YCIVDLHAIT
60 70 80 90 100
VKQEAKDLRR RTLQLIATYI ASGIDPEKNT IFIQSHVPAH VQAQWILNCM
110 120 130 140 150
SYVGELSRMT QFKDKSKKYE DTGIGAGLLN YPVLMAADIL IYQADLVPVG
160 170 180 190 200
KDQTQHIELT RDLAQRFNST YSETFKIPEG YIPTGGAKIM SLQEPLKKMS
210 220 230 240 250
KSSDNPNSFI LIMDPPEVIK RKIARAVTDN VGVVKYTDDQ PGVKNLMNIM
260 270 280 290 300
SCCTGMSVKD IEDKYEGQGY SKFKEDVADA LIQELEPIQN KVNELLKDKA
310 320 330
YLQDICKKGA QKASYIANKT VSKMMRKVGF ILPEK
Length:335
Mass (Da):37,719
Last modified:November 1, 2002 - v2
Checksum:i01B4BD7CF2A04555
GO

Sequence cautioni

The sequence AAK78603 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK78603.1 Different initiation.
PIRiH96976
RefSeqiNP_347263.1, NC_003030.1

Genome annotation databases

EnsemblBacteriaiAAK78603; AAK78603; CA_C0626
GeneIDi1116809
KEGGicac:CA_C0626
PATRICifig|272562.8.peg.828

Similar proteinsi

Entry informationi

Entry nameiSYW_CLOAB
AccessioniPrimary (citable) accession number: Q97LD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 2002
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health