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Q97LD6 (SYW_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:CA_C0626
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAK78603.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B
PRO_0000136621

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140_B
Motif198 – 2025"KMSKS" region HAMAP-Rule MF_00140_B

Sites

Binding site2011ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97LD6 [UniParc].

Last modified November 1, 2002. Version 2.
Checksum: 01B4BD7CF2A04555

FASTA33537,719
        10         20         30         40         50         60 
MGENKKVIFS GIQPSGELTI GNYFGAIKNW VKLQDEYDCY YCIVDLHAIT VKQEAKDLRR 

        70         80         90        100        110        120 
RTLQLIATYI ASGIDPEKNT IFIQSHVPAH VQAQWILNCM SYVGELSRMT QFKDKSKKYE 

       130        140        150        160        170        180 
DTGIGAGLLN YPVLMAADIL IYQADLVPVG KDQTQHIELT RDLAQRFNST YSETFKIPEG 

       190        200        210        220        230        240 
YIPTGGAKIM SLQEPLKKMS KSSDNPNSFI LIMDPPEVIK RKIARAVTDN VGVVKYTDDQ 

       250        260        270        280        290        300 
PGVKNLMNIM SCCTGMSVKD IEDKYEGQGY SKFKEDVADA LIQELEPIQN KVNELLKDKA 

       310        320        330 
YLQDICKKGA QKASYIANKT VSKMMRKVGF ILPEK

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE001437 Genomic DNA. Translation: AAK78603.1. Different initiation.
PIRH96976.
RefSeqNP_347263.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97LD6.
SMRQ97LD6. Positions 6-331.
ModBaseSearch...

Protein-protein interaction databases

STRING272562.CA_C0626.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78603; AAK78603; CA_C0626.
GeneID1116809.
KEGGcac:CA_C0626.
PATRIC32035613. VBICloAce74127_0828.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAHDCLYCV.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycCACE272562:GJIH-667-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_CLOAB
AccessionPrimary (citable) accession number: Q97LD6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 2002
Last modified: May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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