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Q97LB6 (SYL_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CA_C0646
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length812 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 812812Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152002

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif572 – 5765"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5751ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97LB6 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 8556803C1FCFE29C

FASTA81292,889
        10         20         30         40         50         60 
MSTYSTKVDE KWQKKWEETS LYKFDENNLE KKLYVLEMFS YPSGAKLHAG HWFNYAPVDS 

        70         80         90        100        110        120 
WARFKKMTGY NVFQPMGFDA FGLPAENYAI KTGIHPKDST LKNIETMEKQ LKSMGAMFNW 

       130        140        150        160        170        180 
DHEVITCTPD YYKWTQWLFL ELYKNGLAYR KKAPANWCPS CNTVLANEQV VDGSCERCGT 

       190        200        210        220        230        240 
EVIKKDLTQW FFKITNYAEE LLQKLDELDW PEKTKAMQKH WIGKSKGAEV TFKVDNSDLK 

       250        260        270        280        290        300 
FDVFTTRVDT LCGVTYVVLA PESPLADELT KPEYKEKVEE YKLQAQKQSE IERQSLTREK 

       310        320        330        340        350        360 
TGVFTGSYAI NPINNKKVPI WIADYVIYTY GTGAVMAVPS HDERDFAFAT KYDLPIVRVV 

       370        380        390        400        410        420 
EGGEELPFTG YGPLVNSGEF DGLKGNKAKE AIVSKLSEND LGRWKVNYRL RDWLVSRQRY 

       430        440        450        460        470        480 
WGAPIPVVYC EKCGIVPVPE KDLPVELPYN VEFNPTGKSP LTTSEEFMNT TCPHCGGHAT 

       490        500        510        520        530        540 
RESDTLDTFV CSSWYYLRYA DNENSDAPFD KEKISKMLPV DKYVGGPEHA CMHLLYARFI 

       550        560        570        580        590        600 
TKALRDIGYL NFDEPFLSLR HQGLILGPDG QKMSKSKGNT ISPDDIIAEH GSDVFRMYLM 

       610        620        630        640        650        660 
FGFDYAEGGA WSDDGVKAMS KFVDRFSRMI ETAKEEINNP KNSKTDMGKE EKELNYVRNH 

       670        680        690        700        710        720 
SIKAVTEDMD KFQFNTCIAR LMEFTNSLSK YLTVDNKNIK LLKDTTIDII KLIAPFAPHF 

       730        740        750        760        770        780 
AEEQWNIIEE KYSVFNEKWP EFDEKALVKD EVEIAIQING KIKAKINIAT NLSEDEIKSA 

       790        800        810 
ALADDKVKAA TDGKNIVKVI VIKGRLVNIV VK 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK78623.1.
PIRD96979.
RefSeqNP_347283.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97LB6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C0646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78623; AAK78623; CA_C0646.
GeneID1116829.
KEGGcac:CA_C0646.
PATRIC32035655. VBICloAce74127_0849.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCACE272562:GJIH-687-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CLOAB
AccessionPrimary (citable) accession number: Q97LB6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries