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Protein

Histidinol dehydrogenase

Gene

hisD

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.UniRule annotation

Catalytic activityi

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311NADUniRule annotation
Binding sitei193 – 1931NADUniRule annotation
Binding sitei216 – 2161NADUniRule annotation
Binding sitei239 – 2391SubstrateUniRule annotation
Metal bindingi261 – 2611ZincUniRule annotation
Binding sitei261 – 2611SubstrateUniRule annotation
Metal bindingi264 – 2641ZincUniRule annotation
Binding sitei264 – 2641SubstrateUniRule annotation
Active sitei329 – 3291Proton acceptorUniRule annotation
Active sitei330 – 3301Proton acceptorUniRule annotation
Binding sitei330 – 3301SubstrateUniRule annotation
Metal bindingi363 – 3631ZincUniRule annotation
Binding sitei363 – 3631SubstrateUniRule annotation
Binding sitei417 – 4171SubstrateUniRule annotation
Metal bindingi422 – 4221ZincUniRule annotation
Binding sitei422 – 4221SubstrateUniRule annotation

GO - Molecular functioni

  1. histidinol dehydrogenase activity Source: UniProtKB-HAMAP
  2. NAD binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciCACE272562:GJIH-980-MONOMER.
UniPathwayiUPA00031; UER00014.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol dehydrogenaseUniRule annotation (EC:1.1.1.23UniRule annotation)
Short name:
HDHUniRule annotation
Gene namesi
Name:hisDUniRule annotation
Ordered Locus Names:CA_C0937
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Histidinol dehydrogenasePRO_0000135756Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_C0937.

Structurei

3D structure databases

ProteinModelPortaliQ97KI2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidinol dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OMAiLSVQSFL.
OrthoDBiEOG6CVVCR.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97KI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDIIRIIQD GSLDGEKYFQ SLKERQGKEN AEIIKTVKFI IDNVKENGDK
60 70 80 90 100
ALIEYTSKFD KVELQSIEVT KEEIKAAYSK VENDFICALK TAKENIEEYH
110 120 130 140 150
SKQVQNSYVI TKENGIVMGR TVRGLDKVGI YVPGGTAAYP SSVIMNAVPA
160 170 180 190 200
KVAGVNKIIM TTPPMKDGFV NPSILVAADL AGVDKIYKVG GAQAIAALAF
210 220 230 240 250
GTETIDKVDK IVGPGNIFVA MAKKSVYGFV DIDMIAGPSE ILVISDETGN
260 270 280 290 300
PKFIAADLMS QAEHDTLASS ILVTTSKELI GKVIEEIKLQ VEGLSRKEII
310 320 330 340 350
LEALRNFGAI ILVDSISRAI EIGNVVAPEH LEIITPNPFE YLNDIKNAGS
360 370 380 390 400
IFLGSYSPEP LGDYMAGPNH VLPTSGTARF SSPLSVDDFV KKSSYLYYSE
410 420 430
KALRNVNDKV VKIAETEGLT AHANSIKVRF K
Length:431
Mass (Da):46,930
Last modified:October 1, 2001 - v1
Checksum:i70AFA3CB1946DF19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK78913.1.
PIRiF97015.
RefSeqiNP_347573.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78913; AAK78913; CA_C0937.
GeneIDi1117120.
KEGGicac:CA_C0937.
PATRICi32036255. VBICloAce74127_1147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK78913.1.
PIRiF97015.
RefSeqiNP_347573.1. NC_003030.1.

3D structure databases

ProteinModelPortaliQ97KI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C0937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK78913; AAK78913; CA_C0937.
GeneIDi1117120.
KEGGicac:CA_C0937.
PATRICi32036255. VBICloAce74127_1147.

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OMAiLSVQSFL.
OrthoDBiEOG6CVVCR.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00014.
BioCyciCACE272562:GJIH-980-MONOMER.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiHISX_CLOAB
AccessioniPrimary (citable) accession number: Q97KI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 1, 2001
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.