Q97KF6 (UPPP2_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase 2
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein 2
Undecaprenyl pyrophosphate phosphatase 2

Gene names

Name:	uppP2
Synonyms:	bacA2, upk2
Ordered Locus Names:	CA_C0963

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin By similarity. HAMAP-Rule MF_01006
Catalytic activity	Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. HAMAP-Rule MF_01006
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01006.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP-Rule MF_01006
Sequence similarities	Belongs to the UppP family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	undecaprenyl-diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Undecaprenyl-diphosphatase 2 HAMAP-Rule MF_01006		PRO_0000151135
Regions
	Transmembrane	19 – 41	23	Helical; Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q97KF6 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 32FF21C78024F34D	FASTA	307	33,640
	10 20 30 40 50 60 MYTNLIFIFK IILEGIIEGV TEFLPVSSTG HMIILGSIIG FKEGAKPVSL YGAEYIHMFE 70 80 90 100 110 120 IIIQLGAILA IVVLYWDKIF SALKPSNLFP SMKEHEKSGI GVVGEFFVKG YNTMPGFKFW 130 140 150 160 170 180 TNIVVACIPA IVIGLPFQKK IDKLLFFPAP VAAALMVGAV WMIFAENKYR KRAKIKSVDE 190 200 210 220 230 240 ITIKQAIVIG CFQCLALWPG MSRSASTIIG AWIVGVATVA GAEFSFFLAI PMMLGASLLF 250 260 270 280 290 300 LIKNSVVLSS VQILGLAVGF IVAFIVALVV VDRFISFLKK KPMRIFAVYR LAIGIIVLVL GFTKVIS

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK78939.1.
PIR	H97018.
RefSeq	NP_347599.1. NC_003030.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_C0963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK78939; AAK78939; CA_C0963.
GeneID	1117146.
KEGG	cac:CA_C0963.
PATRIC	32036305. VBICloAce74127_1172.
Phylogenomic databases
eggNOG	COG1968.
HOGENOM	HOG000218356.
KO	K06153.
OMA	RWLLRYI.
ProtClustDB	PRK12554.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-1006-MONOMER.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase.
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP2_CLOAB

Accession

Primary (citable) accession number: Q97KF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program