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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CA_C0990
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195441 – 485Glutamate--tRNA ligaseAdd BLAST485

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C0990

Structurei

3D structure databases

ProteinModelPortaliQ97KC9
SMRiQ97KC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q97KC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANEIRTRF APSPTGYMHI GNLRTALYTY LIAKHEDGKF ILRIEDTDQE
60 70 80 90 100
RYVEDALAVI YKTLEMTGLK HDEGPDVGGP VGPYVQSERK GLYLDYAKKL
110 120 130 140 150
VEKGEAYYCF CSKERLDILK TNSEALKRPF KYDKHCANLS KEEVQEKLDA
160 170 180 190 200
GVPYVIRQNN PTTGSTTFDD VIYGRISVDN SELDDMILIK SDGYPTYNFA
210 220 230 240 250
NVVDDHLMGI THVVRGNEYL SSAPKYNRLY EAFGWNVPIY VHCPPIMKDA
260 270 280 290 300
HSKLSKRNGD ASFQDLIEKG YLKEAVLNYI ALLGWNPEGT NEILSLEDMV
310 320 330 340 350
KLFDYTHINK SPAVFDPVKL KWMNGEYVRK LSLDEFHKAA LSYYDGVITK
360 370 380 390 400
ENIDLKKISE LIQTRVEIFS DIPEMVDFFN ELPDYDIEMY THKKMKTNPE
410 420 430 440 450
ISLDSLKNCL PVIENIEDWN LDNIQNTIMN YIKDLGVKNG VVLWPLRTAL
460 470 480
SGKKFTPGGA FEIADIIGKD ESIRRIKIGI EKLEK
Length:485
Mass (Da):55,471
Last modified:October 1, 2001 - v1
Checksum:iB7D08618F9A25697
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK78966.1
PIRiC97022
RefSeqiNP_347626.1, NC_003030.1
WP_010964308.1, NC_003030.1

Genome annotation databases

EnsemblBacteriaiAAK78966; AAK78966; CA_C0990
GeneIDi1117173
KEGGicac:CA_C0990
PATRICifig|272562.8.peg.1199

Similar proteinsi

Entry informationi

Entry nameiSYE_CLOAB
AccessioniPrimary (citable) accession number: Q97KC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2001
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health