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Protein

Quinolinate synthase A

Gene

nadA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231IminoaspartateUniRule annotation
Binding sitei40 – 401Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei111 – 1111IminoaspartateUniRule annotation
Binding sitei128 – 1281IminoaspartateUniRule annotation
Binding sitei197 – 1971IminoaspartateUniRule annotation
Binding sitei214 – 2141IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-1072-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:CA_C1025
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Quinolinate synthase APRO_0000155784Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_C1025.

Structurei

3D structure databases

ProteinModelPortaliQ97K94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.
OrthoDBiEOG6DJZ56.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97K94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQNLKEQIL ELKRQKNAII LAHYYQRPEV QEIADFIGDS YNLSKIAKEN
60 70 80 90 100
DADTIVFCGV KFMAESAKVL SPQKKVLLPQ PKAGCPMADM ADAEGLAALK
110 120 130 140 150
AKHPNAKVVS YINSSVEVKA LCDTCCTSSN AAKIVKRIDS DEIIFLPDKN
160 170 180 190 200
LGSYVQEMVP EKNIILWDGF CKVHNMIIPT DIEEMKNKYG DMKILAHPEC
210 220 230 240 250
WKPVRDMADF IGSTGAMIDY AEKDTTSDKY LVVTETGIMY KMQERVPNKT
260 270 280 290 300
FYPLRSMVCV NMKATHLEDV YNSLVNSTFE INIEENLRQK ALTSLENMLI

LGR
Length:303
Mass (Da):34,190
Last modified:October 1, 2001 - v1
Checksum:i6E4AD28549AD9080
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK79001.1.
PIRiF97026.
RefSeqiNP_347661.1. NC_003030.1.
WP_010964343.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK79001; AAK79001; CA_C1025.
GeneIDi1117208.
KEGGicac:CA_C1025.
PATRICi32036435. VBICloAce74127_1233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK79001.1.
PIRiF97026.
RefSeqiNP_347661.1. NC_003030.1.
WP_010964343.1. NC_003030.1.

3D structure databases

ProteinModelPortaliQ97K94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C1025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK79001; AAK79001; CA_C1025.
GeneIDi1117208.
KEGGicac:CA_C1025.
PATRICi32036435. VBICloAce74127_1233.

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.
OrthoDBiEOG6DJZ56.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciCACE272562:GJIH-1072-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiNADA_CLOAB
AccessioniPrimary (citable) accession number: Q97K94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 1, 2001
Last modified: December 9, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.