Q97JG4 (GLPK_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	CA_C1321

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glycerol kinase HAMAP MF_00186		PRO_0000059443
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	310	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97JG4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2EEFDDD41BB0ACE0	FASTA	498	55,661
	10 20 30 40 50 60 MKKYIIALDQ GTTSSRAIIF DHEQNILEIS QKEFTQIYPS KGWVEHNPLE IWSSQYGVLQ 70 80 90 100 110 120 EVMAKANITQ ENIAAIGITN QRETTIVWDK NTGEPVYNAI VWQCRRTADI VEGLKRDKEF 130 140 150 160 170 180 SDYVKENTGL ILDAYFSATK IKWILDNVEG AREKAEKGEL LFGTVDTWLV WKLTNGKVHV 190 200 210 220 230 240 TDYTNASRTM LYNIKELKWD ERILRKLNIP RSMLPEVKNS SEVYGYTNLG GKGGIRVPIA 250 260 270 280 290 300 GMAGDQQCAL FGQTCFEEGS VKNTYGTGCF LLMNTGENII QSKNGLVTTI AIGLEGKVQY 310 320 330 340 350 360 ALEGSVFVGG AVIQWIRDEL KLVSDAADTE YFAKKVNDNG GVYVVPAFTG LGAPYWDMYA 370 380 390 400 410 420 RGAIFGLTRG ANKNHIIRAA LEAIAYQSRD LIDAMKEDSG CEITRIKVDG GASRNNLLMQ 430 440 450 460 470 480 FQADITGTEV VRPIITETTA LGAAYLAGLA VGFWKSKEEI AEKWSVSEVY TPNLDEDEKI 490 RLYNGWKRAV ERVQGWEE

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK79290.1.
PIR	G97062.
RefSeq	NP_347950.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97JG4.
SMR	Q97JG4. Positions 2-498.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1117504.
GenomeReviews	Gene locus CA_C1321 in contig AE001437_GR.
KEGG	cac:CA_C1321.
NMPDR	fig|272562.1.peg.1479.
PATRIC	32037025. VBICloAce74127_1526.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	SIAYQSN.
PhylomeDB	Q97JG4.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	CACE272562:CAC1321-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_CLOAB

Accession

Primary (citable) accession number: Q97JG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program