Q97JC5 (BIOD_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CA_C1361

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187958
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	115 – 118	4	ATP By similarity
	Nucleotide binding	179 – 180	2	ATP By similarity
	Nucleotide binding	207 – 209	3	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	54	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97JC5 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2FA903A8C23E4AD1	FASTA	240	26,677
	10 20 30 40 50 60 MRGIYLVGTD TEIGKTVISA ALVYILCKAG YKTAYFKAAL SDAEELNGKL IPGDTEFVCS 70 80 90 100 110 120 LSGIQEDYEK LTPYIYKTAV SPYLASKIEN KPIDIQVIKD KLTKLENEYG YVLCEGSGGV 130 140 150 160 170 180 ICPITDVDNK IYTLGNLIKD MKMDVILVCR AGLGTINHTV LTVSYLKNNG IEVKGIIVNQ 190 200 210 220 230 240 YEENRLCKDN INMIKKMTGI PILGVMPYIN ENKKNFIEEL KYNACNIFKA DEIAGLMKEV

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK79329.1.
PIR	F97067.
RefSeq	NP_347989.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97JC5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1117544.
GenomeReviews	Gene locus CA_C1361 in contig AE001437_GR.
KEGG	cac:CA_C1361.
NMPDR	fig|272562.1.peg.1518.
PATRIC	32037105. VBICloAce74127_1566.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	PQMERIE.
PhylomeDB	Q97JC5.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	CACE272562:CAC1361-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOAB

Accession

Primary (citable) accession number: Q97JC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program