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Q97J91 (PUR9_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:CA_C1395
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_0000192084

Sequences

Sequence LengthMass (Da)Tools
Q97J91 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 1E720321A56F4606

FASTA49955,238
        10         20         30         40         50         60 
MIKRALISVF NKNGILKLAK FLNEKGVEIL STGGTYKHLK ENGIPVIEVS EVTGFDEILD 

        70         80         90        100        110        120 
GRVKTLHPKI HGGILAIRDN KEHMDTIKNK GITPIDMVVV NLYPFFDKVK ENISFEEKVE 

       130        140        150        160        170        180 
FIDIGGPTMI RAAAKNFQDV VVLTDVNDYD DVIDQIEKTG EVAYNTKKRL AGKVFNLMSA 

       190        200        210        220        230        240 
YDGAISRFLL EDEYPEYLAV PYKKKMDLRY GENPHQTAAF YEAAFGDGAM KGFEQLNGKE 

       250        260        270        280        290        300 
LSYNNIKDMD IAWKVVNEFD ETVCCALKHN SPCGVAIGEN PLDAYKKAFE CDDTSIFGGI 

       310        320        330        340        350        360 
VALNKVIDKP AAEEMVKIFL EIVIAPDFTA DALEVLKSKK NLRVIKANEK PSDNFELAKV 

       370        380        390        400        410        420 
DGAMLVQSAD NKLTEKMEVV TDKKPTDEEM RDMIFGMKVV KYVKSNAIVV VKNGAAVGIG 

       430        440        450        460        470        480 
GGQVNRIWPA KDAMERGKGA AVLASDAFFP FGDIVEEAHK NGIKAIIQPG GSIRDQESID 

       490 
GCNKYGISMV MTGIRHFKH 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK79363.1.
PIRH97071.
RefSeqNP_348023.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97J91.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C1395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK79363; AAK79363; CA_C1395.
GeneID1117578.
KEGGcac:CA_C1395.
PATRIC32037175. VBICloAce74127_1601.

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMADEIACCA.
OrthoDBEOG6QCDFF.

Enzyme and pathway databases

BioCycCACE272562:GJIH-1444-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_CLOAB
AccessionPrimary (citable) accession number: Q97J91
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways