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Q97J90 (PUR2_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:CA_C1396
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151444

Regions

Domain107 – 313207ATP-grasp
Nucleotide binding133 – 19462ATP By similarity

Sites

Metal binding2831Magnesium or manganese By similarity
Metal binding2851Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97J90 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 5840F01F63800C4D

FASTA41645,754
        10         20         30         40         50         60 
MKILLIGSGG REHAMAWKMA QNKSVERIYC APGNGGTAKE NKCENVNLEK TEELIEFASK 

        70         80         90        100        110        120 
NNIDLTVVGP EAPLVDGIVN EFKEKGLKIF GPGKVGAQLE GSKSFSKDFM KKYNVKTAEY 

       130        140        150        160        170        180 
AVFENSEESL EYLKKCTYPI VIKADGLAAG KGVVICEDYK LAEETIKAFM VKDVFKGSGK 

       190        200        210        220        230        240 
KVVIEEYLEG VEASILSITD GKAIIPFVSS KDHKQIFDGN KGPNTGGMGA ISPNPYCTEE 

       250        260        270        280        290        300 
VLKSFEEEIL KPTLIGIQEE RMDFTGIIFF GLMITKKGVY LLEYNVRLGD PETQVVLYLM 

       310        320        330        340        350        360 
KSDFVDLINA AMDKKLSDFD IEWYDGNACC VVAASKGYPK NYSTGYEISG IDDAGDKVFC 

       370        380        390        400        410 
AGVKLENGVY KTSGGRVLCA SARGITLDEA IKKAYTDIER IKFDGIYYRK DIGKSK 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK79364.1.
PIRA97072.
RefSeqNP_348024.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97J90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C1396.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK79364; AAK79364; CA_C1396.
GeneID1117579.
KEGGcac:CA_C1396.
PATRIC32037177. VBICloAce74127_1602.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycCACE272562:GJIH-1445-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_CLOAB
AccessionPrimary (citable) accession number: Q97J90
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2001
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways