Reviewed, UniProtKB/Swiss-Prot Q97J90 (PUR2_CLOAB)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	CA_C1396

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151444
Regions
	Domain	107 – 313	207	ATP-grasp
	Nucleotide binding	133 – 194	62	ATP By similarity
Sites
	Metal binding	283	1	Magnesium or manganese By similarity
	Metal binding	285	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97J90-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 5840F01F63800C4D	FASTA	416	45,754
	10 20 30 40 50 60 MKILLIGSGG REHAMAWKMA QNKSVERIYC APGNGGTAKE NKCENVNLEK TEELIEFASK 70 80 90 100 110 120 NNIDLTVVGP EAPLVDGIVN EFKEKGLKIF GPGKVGAQLE GSKSFSKDFM KKYNVKTAEY 130 140 150 160 170 180 AVFENSEESL EYLKKCTYPI VIKADGLAAG KGVVICEDYK LAEETIKAFM VKDVFKGSGK 190 200 210 220 230 240 KVVIEEYLEG VEASILSITD GKAIIPFVSS KDHKQIFDGN KGPNTGGMGA ISPNPYCTEE 250 260 270 280 290 300 VLKSFEEEIL KPTLIGIQEE RMDFTGIIFF GLMITKKGVY LLEYNVRLGD PETQVVLYLM 310 320 330 340 350 360 KSDFVDLINA AMDKKLSDFD IEWYDGNACC VVAASKGYPK NYSTGYEISG IDDAGDKVFC 370 380 390 400 410 AGVKLENGVY KTSGGRVLCA SARGITLDEA IKKAYTDIER IKFDGIYYRK DIGKSK

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001437 Genomic DNA. Translation: AAK79364.1.
PIR	A97072.
RefSeq	NP_348024.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1117579.
GenomeReviews	Gene locus CA_C1396 in contig AE001437_GR.
KEGG	cac:CAC1396.
NMPDR	fig|272562.1.peg.1553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97J90.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	CACE272562:CAC1396-MON.
BRENDA	6.3.4.13. 2866.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CLOAB

Accession

Primary (citable) accession number: Q97J90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)