Q97J51 (Y1435_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized RNA methyltransferase CA_C1435
EC=2.1.1.-

Gene names

Ordered Locus Names:

CA_C1435

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. Contains 1 TRAM domain.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA methylation Inferred from electronic annotation. Source: GOC RNA processing Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Uncharacterized RNA methyltransferase CA_C1435		PRO_0000161969
Regions
	Domain	3 – 61	59	TRAM
Sites
	Active site	411	1	Nucleophile By similarity
	Metal binding	74	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	80	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	163	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	289	1	S-adenosyl-L-methionine By similarity
	Binding site	318	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	339	1	S-adenosyl-L-methionine By similarity
	Binding site	384	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97J51 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2F163B07786725A0	FASTA	456	51,312
	10 20 30 40 50 60 MKLMRKNETR EFLIEDIEFP AVGVAFYNDK KVYIKGAVPG QKVLARVSKV RREKIEAKLK 70 80 90 100 110 120 EIVTNIPGAA QPKCPDFGVC GGCVHQFLPY EKQLEFKERE VLKLFKDAKI EGFEYLGILG 130 140 150 160 170 180 SPEKEEYRNK MEYTFGDFVK GGELTLGMHA KNSGFSIVNT DKCNIVDEDF RIILKTVVEY 190 200 210 220 230 240 FRKKDLPIYK VMQHVGYLRN LVVRKAKNTG EILIALVTTS QVDFDLTELT EILKSINYLG 250 260 270 280 290 300 ELKGILHVIN DGLADMVRGD KIVTLFGQDY ITERILDLKF KISLFSFFQT NSKGAEKLYS 310 320 330 340 350 360 EVLEFLGDVS NKTVFDLYCG TGTIGQLASK KAEKVIGIEL IEEAVEAAKE NTKLNNISNC 370 380 390 400 410 420 SFIAGDVAKV ITEIKEKPDT IILDPPRPGV SPNAMKYVIK FNAPEIVYVS CNPKTLVNDL 430 440 450 GVLRAYGYEV EKVKIVDMFP GTGHVETVVL LQRKII

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK79403.1.
PIR	H97076.
RefSeq	NP_348063.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97J51.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_C1435.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK79403; AAK79403; CA_C1435.
GeneID	1117618.
KEGG	cac:CA_C1435.
PATRIC	32037253. VBICloAce74127_1640.
Phylogenomic databases
eggNOG	COG2265.
HOGENOM	HOG000029870.
KO	K00599.
OMA	MSDYTQQ.
ProtClustDB	CLSK882798.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-1484-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
InterPro	IPR001566. 23S_rRNA_MeTrfase_RlmD. IPR012340. NA-bd_OB-fold. IPR002792. TRAM_dom. IPR010280. U5_MeTrfase. [Graphical view]
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00479. rumA. 1 hit.
PROSITE	PS50926. TRAM. 1 hit. PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1435_CLOAB

Accession

Primary (citable) accession number: Q97J51

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program