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Q97HD7 (NADK_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:CA_C2075
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284NAD kinase HAMAP-Rule MF_00361
PRO_0000120611

Regions

Nucleotide binding61 – 622NAD By similarity
Nucleotide binding134 – 1352NAD By similarity
Nucleotide binding175 – 1806NAD By similarity

Sites

Active site611Proton acceptor By similarity
Binding site1451NAD By similarity
Binding site1621NAD By similarity
Binding site1641NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97HD7 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 253795F8FBE6BD48

FASTA28432,026
        10         20         30         40         50         60 
MKNIGVNINS CKDPDGTIRK YVEKVIKEEK KDVQVKFYDE LNYFDEVCKT KPDFFIAFGG 

        70         80         90        100        110        120 
DGTILNAARN LVSCGIPIFS VNIGHLGFLS SIEFKDFKDA IHKIFKGEYF FQERTMLKCS 

       130        140        150        160        170        180 
FIKGNSKKVF YSLNEVVLYK GNMAKILKYN IDVDDKFYMG FKSDGIIIST PTGSTAYNLS 

       190        200        210        220        230        240 
AGGPIIYPNL DLISLTPICP QGPYAGTIVL DGKSNITISG IDANENVFIT VDGRQPVDVK 

       250        260        270        280 
GVSFIEISKL NYKCKLLKLK DYNYFEVLRK KIILRTEECE GDKY 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK80034.1.
PIRG97155.
RefSeqNP_348694.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97HD7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C2075.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK80034; AAK80034; CA_C2075.
GeneID1118258.
KEGGcac:CA_C2075.
PATRIC32038533. VBICloAce74127_2279.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMARWEALCL.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycCACE272562:GJIH-2124-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_CLOAB
AccessionPrimary (citable) accession number: Q97HD7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 1, 2001
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families