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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi457 – 4571MagnesiumUniRule annotation
Metal bindingi463 – 4631Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi466 – 4661MagnesiumUniRule annotation
Metal bindingi467 – 4671MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-2405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:CA_C2356
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 792792Phenylalanine--tRNA ligase beta subunitPRO_0000126870Add
BLAST

Proteomic databases

PRIDEiQ97GL0.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C2356.

Structurei

3D structure databases

ProteinModelPortaliQ97GL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 150112tRNA-bindingUniRule annotationAdd
BLAST
Domaini404 – 47976B5UniRule annotationAdd
BLAST
Domaini699 – 79294FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97GL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVPVKWLKD YVDFDINAKE LGDRLTLSGS KVEEIITSGD EITNVVTGKI
60 70 80 90 100
LKIDPHPDAE KLVICSVDVG KNEPIQIVTG AQNMKENDIV PVALHGSTLP
110 120 130 140 150
GGVKIKKGKL RGVVSNGMMC AKEELGIADE EHVHGLMILD ENTPIGKDIK
160 170 180 190 200
EVLGLDNPVI DFEITSNRPD CLSVIGIARE TAATINTKYR NVKIDFSETK
210 220 230 240 250
GRNIKEELEV EVKDKLCRRY MARVIKNVKI EDSPAWMQER LMLAGVRPIN
260 270 280 290 300
NIVDITNFVM LEVGQPMHAF DKKMITSNKI VIERAKDGEK FTTLDSEERS
310 320 330 340 350
LDSNVLMIKD GDKNCAIAGI MGGLDSEVAE NTHEIIFESA NFDGTNIRVS
360 370 380 390 400
SQKLALRTEA SGRYEKDLDP NLAEIALNRA CTLIQELNAG EIVEGVIDIY
410 420 430 440 450
PVKSEPNIVE VDYNWINNFL GIKISKEEMK EYLDRLELTT EIKGDKLEVF
460 470 480 490 500
SPTFRCDINI KEDVAEEVAR IYGYNKVPST TVKSQSIRTG KSKIQQIKDV
510 520 530 540 550
VTDILISSGL NESINYSFVS PKIFDKILVP EDSELRNVVK IRNPLGEDFS
560 570 580 590 600
VMRTTTLHSM MESLARNYSH NNELAKLFEI GKVYIPSENE GEIPKERNVI
610 620 630 640 650
TIGMYGNVDY FDLKGVVENL VEILGVNKIS YARESENPTF HPGKTAVIKI
660 670 680 690 700
KNTVLGTLGE VHPDVCENYE VEERCYVAEI DLDLLLENVS LSRKYKALPK
710 720 730 740 750
FPTVTRDISV LVDEDILVQE IENVIKRQGG TILESLNLFD VYKGKQVPQG
760 770 780 790
KKSVSYALTY RDENKTLTDK DVEKIQNKVI KTLEHVLGAE LR
Length:792
Mass (Da):88,781
Last modified:September 30, 2001 - v1
Checksum:i9BD754AA12E6707D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK80312.1.
PIRiE97190.
RefSeqiNP_348972.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK80312; AAK80312; CA_C2356.
GeneIDi1118539.
KEGGicac:CA_C2356.
PATRICi32039079. VBICloAce74127_2552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK80312.1.
PIRiE97190.
RefSeqiNP_348972.1. NC_003030.1.

3D structure databases

ProteinModelPortaliQ97GL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C2356.

Proteomic databases

PRIDEiQ97GL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK80312; AAK80312; CA_C2356.
GeneIDi1118539.
KEGGicac:CA_C2356.
PATRICi32039079. VBICloAce74127_2552.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-2405-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiSYFB_CLOAB
AccessioniPrimary (citable) accession number: Q97GL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2003
Last sequence update: September 30, 2001
Last modified: January 6, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.