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Q97GL0

- SYFB_CLOAB

UniProt

Q97GL0 - SYFB_CLOAB

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Protein
Phenylalanine--tRNA ligase beta subunit
Gene
pheT, CA_C2356
Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Binds 2 magnesium ions per tetramer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi457 – 4571Magnesium By similarity
Metal bindingi463 – 4631Magnesium; via carbonyl oxygen By similarity
Metal bindingi466 – 4661Magnesium By similarity
Metal bindingi467 – 4671Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-2405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:pheT
Ordered Locus Names:CA_C2356
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 792792Phenylalanine--tRNA ligase beta subunitUniRule annotation
PRO_0000126870Add
BLAST

Proteomic databases

PRIDEiQ97GL0.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi272562.CA_C2356.

Structurei

3D structure databases

ProteinModelPortaliQ97GL0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 150112tRNA-binding
Add
BLAST
Domaini404 – 47976B5
Add
BLAST
Domaini699 – 79294FDX-ACB
Add
BLAST

Sequence similaritiesi

Contains 1 B5 domain.
Contains 1 FDX-ACB domain.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiAISSTFC.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97GL0-1 [UniParc]FASTAAdd to Basket

« Hide

MKVPVKWLKD YVDFDINAKE LGDRLTLSGS KVEEIITSGD EITNVVTGKI    50
LKIDPHPDAE KLVICSVDVG KNEPIQIVTG AQNMKENDIV PVALHGSTLP 100
GGVKIKKGKL RGVVSNGMMC AKEELGIADE EHVHGLMILD ENTPIGKDIK 150
EVLGLDNPVI DFEITSNRPD CLSVIGIARE TAATINTKYR NVKIDFSETK 200
GRNIKEELEV EVKDKLCRRY MARVIKNVKI EDSPAWMQER LMLAGVRPIN 250
NIVDITNFVM LEVGQPMHAF DKKMITSNKI VIERAKDGEK FTTLDSEERS 300
LDSNVLMIKD GDKNCAIAGI MGGLDSEVAE NTHEIIFESA NFDGTNIRVS 350
SQKLALRTEA SGRYEKDLDP NLAEIALNRA CTLIQELNAG EIVEGVIDIY 400
PVKSEPNIVE VDYNWINNFL GIKISKEEMK EYLDRLELTT EIKGDKLEVF 450
SPTFRCDINI KEDVAEEVAR IYGYNKVPST TVKSQSIRTG KSKIQQIKDV 500
VTDILISSGL NESINYSFVS PKIFDKILVP EDSELRNVVK IRNPLGEDFS 550
VMRTTTLHSM MESLARNYSH NNELAKLFEI GKVYIPSENE GEIPKERNVI 600
TIGMYGNVDY FDLKGVVENL VEILGVNKIS YARESENPTF HPGKTAVIKI 650
KNTVLGTLGE VHPDVCENYE VEERCYVAEI DLDLLLENVS LSRKYKALPK 700
FPTVTRDISV LVDEDILVQE IENVIKRQGG TILESLNLFD VYKGKQVPQG 750
KKSVSYALTY RDENKTLTDK DVEKIQNKVI KTLEHVLGAE LR 792
Length:792
Mass (Da):88,781
Last modified:October 1, 2001 - v1
Checksum:i9BD754AA12E6707D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK80312.1.
PIRiE97190.
RefSeqiNP_348972.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK80312; AAK80312; CA_C2356.
GeneIDi1118539.
KEGGicac:CA_C2356.
PATRICi32039079. VBICloAce74127_2552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001437 Genomic DNA. Translation: AAK80312.1 .
PIRi E97190.
RefSeqi NP_348972.1. NC_003030.1.

3D structure databases

ProteinModelPortali Q97GL0.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272562.CA_C2356.

Proteomic databases

PRIDEi Q97GL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK80312 ; AAK80312 ; CA_C2356 .
GeneIDi 1118539.
KEGGi cac:CA_C2356.
PATRICi 32039079. VBICloAce74127_2552.

Phylogenomic databases

eggNOGi COG0072.
HOGENOMi HOG000292087.
KOi K01890.
OMAi AISSTFC.
OrthoDBi EOG6CCH1J.

Enzyme and pathway databases

BioCyci CACE272562:GJIH-2405-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPi MF_00283. Phe_tRNA_synth_beta1.
InterProi IPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view ]
Pfami PF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view ]
SMARTi SM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view ]
SUPFAMi SSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
PROSITEi PS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiSYFB_CLOAB
AccessioniPrimary (citable) accession number: Q97GL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi