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Q97GL0

- SYFB_CLOAB

UniProt

Q97GL0 - SYFB_CLOAB

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Metal bindingi463 – 4631Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi466 – 4661MagnesiumUniRule annotation
    Metal bindingi467 – 4671MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-2405-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Short name:
    PheRSUniRule annotation
    Gene namesi
    Name:pheTUniRule annotation
    Ordered Locus Names:CA_C2356
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 792792Phenylalanine--tRNA ligase beta subunitPRO_0000126870Add
    BLAST

    Proteomic databases

    PRIDEiQ97GL0.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi272562.CA_C2356.

    Structurei

    3D structure databases

    ProteinModelPortaliQ97GL0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 150112tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini404 – 47976B5UniRule annotationAdd
    BLAST
    Domaini699 – 79294FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0072.
    HOGENOMiHOG000292087.
    KOiK01890.
    OMAiAISSTFC.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q97GL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVPVKWLKD YVDFDINAKE LGDRLTLSGS KVEEIITSGD EITNVVTGKI    50
    LKIDPHPDAE KLVICSVDVG KNEPIQIVTG AQNMKENDIV PVALHGSTLP 100
    GGVKIKKGKL RGVVSNGMMC AKEELGIADE EHVHGLMILD ENTPIGKDIK 150
    EVLGLDNPVI DFEITSNRPD CLSVIGIARE TAATINTKYR NVKIDFSETK 200
    GRNIKEELEV EVKDKLCRRY MARVIKNVKI EDSPAWMQER LMLAGVRPIN 250
    NIVDITNFVM LEVGQPMHAF DKKMITSNKI VIERAKDGEK FTTLDSEERS 300
    LDSNVLMIKD GDKNCAIAGI MGGLDSEVAE NTHEIIFESA NFDGTNIRVS 350
    SQKLALRTEA SGRYEKDLDP NLAEIALNRA CTLIQELNAG EIVEGVIDIY 400
    PVKSEPNIVE VDYNWINNFL GIKISKEEMK EYLDRLELTT EIKGDKLEVF 450
    SPTFRCDINI KEDVAEEVAR IYGYNKVPST TVKSQSIRTG KSKIQQIKDV 500
    VTDILISSGL NESINYSFVS PKIFDKILVP EDSELRNVVK IRNPLGEDFS 550
    VMRTTTLHSM MESLARNYSH NNELAKLFEI GKVYIPSENE GEIPKERNVI 600
    TIGMYGNVDY FDLKGVVENL VEILGVNKIS YARESENPTF HPGKTAVIKI 650
    KNTVLGTLGE VHPDVCENYE VEERCYVAEI DLDLLLENVS LSRKYKALPK 700
    FPTVTRDISV LVDEDILVQE IENVIKRQGG TILESLNLFD VYKGKQVPQG 750
    KKSVSYALTY RDENKTLTDK DVEKIQNKVI KTLEHVLGAE LR 792
    Length:792
    Mass (Da):88,781
    Last modified:October 1, 2001 - v1
    Checksum:i9BD754AA12E6707D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK80312.1.
    PIRiE97190.
    RefSeqiNP_348972.1. NC_003030.1.

    Genome annotation databases

    EnsemblBacteriaiAAK80312; AAK80312; CA_C2356.
    GeneIDi1118539.
    KEGGicac:CA_C2356.
    PATRICi32039079. VBICloAce74127_2552.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK80312.1 .
    PIRi E97190.
    RefSeqi NP_348972.1. NC_003030.1.

    3D structure databases

    ProteinModelPortali Q97GL0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_C2356.

    Proteomic databases

    PRIDEi Q97GL0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK80312 ; AAK80312 ; CA_C2356 .
    GeneIDi 1118539.
    KEGGi cac:CA_C2356.
    PATRICi 32039079. VBICloAce74127_2552.

    Phylogenomic databases

    eggNOGi COG0072.
    HOGENOMi HOG000292087.
    KOi K01890.
    OMAi AISSTFC.
    OrthoDBi EOG6CCH1J.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-2405-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiSYFB_CLOAB
    AccessioniPrimary (citable) accession number: Q97GL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3