Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q97GL0 (SYFB_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS
Gene names
Name:pheT
Ordered Locus Names:CA_C2356
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length792 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283

Cofactor

Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00283

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00283.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.

Contains 1 B5 domain.

Contains 1 FDX-ACB domain.

Contains 1 tRNA-binding domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 792792Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283
PRO_0000126870

Regions

Domain39 – 150112tRNA-binding
Domain404 – 47976B5
Domain699 – 79294FDX-ACB

Sites

Metal binding4571Magnesium By similarity
Metal binding4631Magnesium; via carbonyl oxygen By similarity
Metal binding4661Magnesium By similarity
Metal binding4671Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97GL0 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 9BD754AA12E6707D

FASTA79288,781
        10         20         30         40         50         60 
MKVPVKWLKD YVDFDINAKE LGDRLTLSGS KVEEIITSGD EITNVVTGKI LKIDPHPDAE 

        70         80         90        100        110        120 
KLVICSVDVG KNEPIQIVTG AQNMKENDIV PVALHGSTLP GGVKIKKGKL RGVVSNGMMC 

       130        140        150        160        170        180 
AKEELGIADE EHVHGLMILD ENTPIGKDIK EVLGLDNPVI DFEITSNRPD CLSVIGIARE 

       190        200        210        220        230        240 
TAATINTKYR NVKIDFSETK GRNIKEELEV EVKDKLCRRY MARVIKNVKI EDSPAWMQER 

       250        260        270        280        290        300 
LMLAGVRPIN NIVDITNFVM LEVGQPMHAF DKKMITSNKI VIERAKDGEK FTTLDSEERS 

       310        320        330        340        350        360 
LDSNVLMIKD GDKNCAIAGI MGGLDSEVAE NTHEIIFESA NFDGTNIRVS SQKLALRTEA 

       370        380        390        400        410        420 
SGRYEKDLDP NLAEIALNRA CTLIQELNAG EIVEGVIDIY PVKSEPNIVE VDYNWINNFL 

       430        440        450        460        470        480 
GIKISKEEMK EYLDRLELTT EIKGDKLEVF SPTFRCDINI KEDVAEEVAR IYGYNKVPST 

       490        500        510        520        530        540 
TVKSQSIRTG KSKIQQIKDV VTDILISSGL NESINYSFVS PKIFDKILVP EDSELRNVVK 

       550        560        570        580        590        600 
IRNPLGEDFS VMRTTTLHSM MESLARNYSH NNELAKLFEI GKVYIPSENE GEIPKERNVI 

       610        620        630        640        650        660 
TIGMYGNVDY FDLKGVVENL VEILGVNKIS YARESENPTF HPGKTAVIKI KNTVLGTLGE 

       670        680        690        700        710        720 
VHPDVCENYE VEERCYVAEI DLDLLLENVS LSRKYKALPK FPTVTRDISV LVDEDILVQE 

       730        740        750        760        770        780 
IENVIKRQGG TILESLNLFD VYKGKQVPQG KKSVSYALTY RDENKTLTDK DVEKIQNKVI 

       790 
KTLEHVLGAE LR 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK80312.1.
PIRE97190.
RefSeqNP_348972.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97GL0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C2356.

Proteomic databases

PRIDEQ97GL0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK80312; AAK80312; CA_C2356.
GeneID1118539.
KEGGcac:CA_C2356.
PATRIC32039079. VBICloAce74127_2552.

Phylogenomic databases

eggNOGCOG0072.
HOGENOMHOG000292087.
KOK01890.
OMAMKFSEQW.
OrthoDBEOG6CCH1J.
ProtClustDBPRK00629.

Enzyme and pathway databases

BioCycCACE272562:GJIH-2405-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPMF_00283. Phe_tRNA_synth_beta1.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsTIGR00472. pheT_bact. 1 hit.
PROSITEPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFB_CLOAB
AccessionPrimary (citable) accession number: Q97GL0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2001
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries