Q97GH8 (ARGB_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	CA_C2389

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112607
Regions
	Region	61 – 62	2	Substrate binding By similarity
Sites
	Binding site	83	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Site	26	1	Transition state stabilizer By similarity
	Site	245	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97GH8 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 733991B20B8F35CE	FASTA	295	32,661
	10 20 30 40 50 60 MKKMQEPIED PLSKLTQYSG KTFVIKYGGS IMKNKKAEEA FIKDVKYLRK LGINIVIVHG 70 80 90 100 110 120 GGPEISRWLE LSGIESRFVD GLRVTDEKVI EIVQMVLSGK INKKLSLQFN IDGVNAVGLS 130 140 150 160 170 180 GVDNKLIEAT KKYVYKGNET IDIGYVGKVT KVNSEFIKEL LKGGQVPVIA PIGCDNKGNV 190 200 210 220 230 240 YNINADYAAA FISSALDAEK LIILTDVEGV YRNINDPQSI IHEIDIKDVN YYIKEEIIKG 250 260 270 280 290 GMIPKVQCCA SAIENGTKNV QLIDGRNDHC LINDILNYRG TIISYRSGVK CQKAI

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK80344.1.
PIR	E97194.
RefSeq	NP_349004.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97GH8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1118572.
GenomeReviews	Gene locus CA_C2389 in contig AE001437_GR.
KEGG	cac:CA_C2389.
NMPDR	fig|272562.1.peg.2533.
PATRIC	32039157. VBICloAce74127_2586.
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	Q97GH8.
ProtClustDB	CLSK559235.
Enzyme and pathway databases
BioCyc	CACE272562:CAC2389-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_CLOAB

Accession

Primary (citable) accession number: Q97GH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program