Reviewed, UniProtKB/Swiss-Prot Q97FT2 (CARA_CLOAB)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	CA_C2645

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112267
Regions
	Domain	171 – 351	181	Glutamine amidotransferase type-1
	Region	1 – 167	167	CPSase HAMAP MF_01209
Sites
	Active site	246	1	Nucleophile By similarity
	Active site	331	1	By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97FT2-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: D7D5FC1E501ADD7C	FASTA	351	38,836
	10 20 30 40 50 60 MKGIIYLEDG TVFYGTGFGK EGNEFGEIVF NTSMTGYQEI LTDPSYAGQI INMTYPLIGN 70 80 90 100 110 120 YGVNESVNQS KCVYAKGFIV KNIDENPSNY TSEINIDQML KNMGVVGVFG VDTRSITKKI 130 140 150 160 170 180 RSYGTMKCII SNGELSIEEL KKMMDASNIV DDYVNTVSTK EIIHIAGNGN KVAVMDFGIK 190 200 210 220 230 240 NDIIENLKER QCDITIFPYD TDYNEILNIN PDGIFLSNGP GDPKSIPEAI ENVKKLIGKR 250 260 270 280 290 300 PMFGICLGHQ IIALASGGNT YKLKYGHRGG NHGIYDVQRD KAYITAQNHG YAVDEESVLE 310 320 330 340 350 NGMIITHRNL NDGTVEGMKH KSIPLFSVQF HPEGAPGPTD TTYLFDQFVN L

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001437 Genomic DNA. Translation: AAK80592.1.
PIR	E97225.
RefSeq	NP_349252.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1118828.
GenomeReviews	Gene locus CA_C2645 in contig AE001437_GR.
KEGG	cac:CAC2645.
NMPDR	fig|272562.1.peg.2781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97FT2.
OMA	EADIPFF.
Enzyme and pathway databases
BioCyc	CACE272562:CAC2645-MON.
BRENDA	6.3.5.5. 2866.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CLOAB

Accession

Primary (citable) accession number: Q97FT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)